Glucose metabolism in mammalian cell culture: new insights for tweaking vintage pathways

Effect of glucose availability on glucose transport in bovine mammary epithelial cells

“…In the same pattern, high concentrations of glucose enhanced BMEC proliferation. The intermediary metabolites of glucose, such as glucose-6-phosphate and NADPH can act as survival factors (Plas et al, 2002;Nutt et al, 2005), and cell growth is dependent on energy supply (Buchakjian and Kornbluth, 2010;Mulukutla et al, 2010). It is indicated, from our results, that glucose availability did affect the glucose transport and utilization by the BMEC, which is not consistent with the report that glucose uptake is unaffected by glucose infusion in ruminants.…”

“…Besides, inhibition of HK2 may activate mitochondrial apoptotic signal cascades and induce apoptotic cell death (Pastorino et al, 2002;Pastorino and Hoek, 2003). The depressed growth of cells may partly account for the depression of glucose uptake, because glucose is required for cell growth (Greiner et al, 1994;Mulukutla et al, 2010). However, the specific glucose-sensing mechanism and pathways in the BMEC are yet to be elucidated in detail.…”

The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody

“…3.1.1), producing a lactate byproduct. Under normal conditions, mammalian cells display an inefficient metabolic phenotype, characterized by high rates of glucose-to-lactate conversion, in which nearly 90% of glucose is converted to lactate during aerobic glycolysis (Mulukutla et al, 2010).…”

“…Cellular engineering attempts to manipulate glucose metabolism through (1) constraining glycolytic fluxes through reaction networking; (2) regulation of metabolic enzymes; and (3) regulation of metabolism by signaling proteins and growth control elements (Mulukutla et al, 2010). However, high oxidative metabolism associates with slow cell growth or no growth, which is commonly observed at the late stage of cell culture (Mulukutla et al, 2010).…”

“…However, high oxidative metabolism associates with slow cell growth or no growth, which is commonly observed at the late stage of cell culture (Mulukutla et al, 2010). consumed by the cells as glucose (Mulukutla et al, 2010).…”