Primary bovine mammary epithelial cells (BMEC) were cultured in media containing varying concentrations of glucose, to determine the effects of glucose availability on glucose transport and its mechanism in bovine mammary gland. The BMEC incubated with 10 and 20 mM glucose had twofold greater glucose uptake than that with 2.5 mM glucose (P , 0.05). Increased glucose availability enhanced the cell proliferation (P , 0.05). As the glucose uptake is mediated by facilitative glucose transporters (GLUTs), the expression of GLUT mRNA was investigated. Compared with the control (2.5 mM), 5 and 10 mM glucose did not influence the abundance of GLUT1 mRNA (P , 0.05), whereas 20 mM glucose decreased the GLUT1 mRNA expression in the BMEC (P , 0.05). The expression of GLUT8 mRNA was not affected by any concentration of glucose (P . 0.05). As GLUTs are coupled with hexokinases (HKs) in regulating glucose uptake, the expression of HKs and their activities were also studied. The HK activity was greater in 5, 10 and 20 mM glucose than that in 2.5 mM glucose (P , 0.05). The expression of HK2 mRNA rather than HK1 mRNA was detected in the BMEC; however, the abundance of HK2 mRNA was not elevated by any concentrations of glucose compared with control (P . 0.05). Furthermore, addition of 3-bromopyruvate (30, 50 or 70 mM), an inhibitor of HK2, resulted in the decrease of glucose uptake and cell proliferation at both 2.5 and 10 mM glucose (P , 0.05). Therefore, the glucose concentrations may affect glucose uptake partly by altering the activity of HKs, and HK2 may play an important role in the regulation of glucose uptake in the BMEC.

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The effect of feeding brittle culm1 whole crop rice (WCR) replacing wheat by-products on performance, digestibility and carcass quality was evaluated in growing-finishing pigs. Fifty-four pigs (Duroc) were randomly assigned to three dietary treatments: WCR at levels of 0 (control), 10 or 20%, respectively. Pigs were fed from about 29 to 85 kg. A digestion trial was conducted with all pigs at about 60 kg live weight. When the feeding trial was completed, six pigs from each treatment were slaughtered to determine carcass quality. In the growing period, pigs receiving the 10 or 20% WCR diet were not significantly different in daily feed intake, daily weight gain and feed conversion from the control pigs (p /0.05). In the finishing period, daily feed intake and daily weight gain of pigs fed the 20% WCR diet were significantly lower than control pigs or 10% WCR diet (p B/0.05). However, the feed conversion was not significantly different among the pigs on three different diets. No significant (p /0.05) difference was shown in the apparent nutrient digestibility with the WCR substituting for wheat by-products. The dressing percentage and mean backfat thickness tended to be lower in pigs fed the 10 or 20% WCR diet than in control pigs. The meat from pigs on the 20% WCR diet showed a higher pH value at 24 h post mortem, and higher protein and lower fat content in longissimus muscles than control pigs (p B/0.05). This study indicated that WCR could be an alternative feed source to replace wheat by-products in growing-finishing pig diets, but a high proportion of the WCR may result in an adverse effect on the performance traits.

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