Glucose limitation and glucose uptake rate determines metabolite production and sporulation in high cell density continuous cultures of Bacillus amyloliquefaciens 83

Cristiano-Fajardo, Sergio Andrés; Flores, Celia; Flores, Noemí; Tinoco‐Valencia, Raunel; Serrano‐Carreón, Leobardo; Galindo, Enrique

doi:10.1016/j.jbiotec.2019.04.027

Cited by 12 publications

(31 citation statements)

References 38 publications

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“…Ciprofloxacin induces mutations in staphylococcal genes, leading to the resistance evolution and a decline in growth performance (Dengler Haunreiter et al, 2019). Folate metabolism is a carbon metabolism, and carbon metabolism directly affects the growth characteristics of bacteria (Cristiano-Fajardo et al, 2019). Therefore, carbon metabolism, the important metabolic pathway of microbes, may be related to the evolution of antibiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

Changes in the Carbon Metabolism of Escherichia coli During the Evolution of Doxycycline Resistance

Yang

Liang

et al. 2019

Front. Microbiol.

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Despite our continuous improvement in understanding the evolution of antibiotic resistance, the changes in the carbon metabolism during the evolution of antibiotic resistance remains unclear. To investigate the evolution of antibiotic resistance and the changes in carbon metabolism under antibiotic pressure, Escherichia coli K-12 was evolved for 38 passages under a concentration gradient of doxycycline (DOX). The 0th-passage sensitive strain W0, the 20th-passage moderately resistant strain M20, and the 38th-passage highly resistant strain E38 were selected for the determination of biofilm formation, colony area, and carbon metabolism levels, as well as genome and transcriptome sequencing. The MIC of DOX with E. coli significantly increased from 4 to 96 μg/ml, and the IC50 increased from 2.18 ± 0.08 to 64.79 ± 0.75 μg/ml after 38 passages of domestication. Compared with the sensitive strain W0, the biofilm formation amount of the resistant strains M20 and E38 was significantly increased (p < 0.05). Single-nucleotide polymorphisms (SNPs) were distributed in antibiotic resistance-related genes such as ribosome targets, cell membranes, and multiple efflux pumps. In addition, there were no mutated genes related to carbon metabolism. However, the genes involved in the biosynthesis of secondary metabolites and carbon metabolism pathway were downregulated, showing a significant decrease in the metabolic intensity of 23 carbon sources (p < 0.05). The results presented here show that there may be a correlation between the evolution of E. coli DOX resistance and the decrease of carbon metabolism, and the mechanism was worthy of further research, providing a theoretical basis for the prevention and control of microbial resistance.

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Section: Introductionmentioning

confidence: 99%

Changes in the Carbon Metabolism of Escherichia coli During the Evolution of Doxycycline Resistance

Yang

Liang

et al. 2019

Front. Microbiol.

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“…We know that the gene cluster ( pgdS , pgsEACB ) involved in the synthesis γ-PGA are present in both strains B. velezensis 83 and B. velezensis FZB42 and they are of high identity (98%). Nevertheless, is known that B. velezensis 83 is a glutamic acid independent γ-PGA producing strain (Cristiano-Fajardo et al 2019 ) as other few Bacillus strains ( B. subtilis C10, Bacillus licheniformis A13, B. licheniformis TISTR 1010, B. methylotrophicus , B. subtilis NX2, B. amyloliquefaciens LL3) which only need glucose and NH 4 Cl as carbon and nitrogen sources, respectively, for γ-PGA synthesis (Cao et al 2011 ; Hsueh et al 2017 ; Sha et al 2019 ). In contrast, lack of γ-PGA production by B. velezensis FZB42 could be explained due to a glutamate dependent mechanism.…”

Section: Resultsmentioning

confidence: 99%

“…A 1 mL sample was collected from the shaken flask and bacterial cells were removed from the medium by centrifugation (10,000 × g , 15 min) and filtration through a 0.2 µm membrane (hydrophilic and nonpyrogenic, Sartorious AG, Goettingen, Germany). Glucose, acetoin and butanediol in the supernatant were analyzed by high resolution reverse phase high-performance liquid chromatography (HPLC) using methodology reported previously (Cristiano-Fajardo et al 2019 ) with minor modifications. Briefly, 20 µl of the sample was loaded to an Aminex HPX-87H column (7.8 × 300 mm; Bio-Rad Laboratories Inc., California, USA) and separated by using a Waters 2695 HPLC system (Waters Corporation, Massachusetts, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Concentration of γ-PGA and mean molecular weight (MW) in the supernatant was determined by gel permeation chromatography (GPC) using methodology reported previously (Cristiano-Fajardo et al 2019 ). Summarizing, 100 µl of cell-free supernatant was loaded into serial connected Ultrahydrogel columns (UG500/Linear; Waters Corporation, Massachusetts, USA), using a Waters 2695 HPLC system.…”

Section: Methodsmentioning

confidence: 99%

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Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion

et al. 2020

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Bacillus velezensis 83 was isolated from mango tree phyllosphere of orchards located in El Rosario, Sinaloa, México. The assessment of this strain as BCA (biological control agent), as well as PGPB (plant growth-promoting bacteria), were demonstrated through in vivo and in vitro assays. In vivo assays showed that B. velezensis 83 was able to control anthracnose (Kent mangoes) as efficiently as chemical treatment with Captan 50 PH™ or Cupravit hidro™. The inoculation of B. velezensis 83 to the roots of maize seedlings yielded an increase of 12% in height and 45% of root biomass, as compared with uninoculated seedlings. In vitro co-culture assays showed that B. velezensis 83 promoted Arabidopsis thaliana growth (root and shoot biomass) while, under the same experimental conditions, B. velezensis FZB42 (reference strain) had a suppressive effect on plant growth. In order to characterize the isolated strain, the complete genome sequence of B. velezensis 83 is reported. Its circular genome consists of 3,997,902 bp coding to 3949 predicted genes. The assembly and annotation of this genome revealed gene clusters related with plant-bacteria interaction and sporulation, as well as ten secondary metabolites biosynthetic gene clusters implicated in the biological control of phytopathogens. Despite the high genomic identity (> 98%) between B. velezensis 83 and B. velezensis FZB42, they are phenotypically different. Indeed, in vitro production of compounds such as surfactin and bacillomycin D (biocontrol activity) and γ-PGA (biofilm component) is significantly different between both strains.

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“…[3][4][5] The increase in the apparent viscosity, due to the production of PGA, reduced considerably the oxygen transfer. 6 This is basically due to the reduction in the turbulence intensity, an increase in the thickness of the fluid boundary around bubble layers, leading to a higher resistance to oxygen transfer, and the promotion of coalescence. 7 At the same time, the bacteria produce significant amounts of surfactants (bacillomycin and surfactin) which increase the interfacial surface area.…”

Section: Introductionmentioning

confidence: 99%

Oxygen transfer and bubble sizes occurring in a pilot‐scale cultivation of Bacillus velezensis 83 for the production of poly(γ‐glutamic acid) under two schemes of power drawn

Pulido‐Durán

Flores

Serrano‐Carreón

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND The influence of power consumption on oxygen transfer during non‐Newtonian and surfactant‐producing microbial fermentations has been scarcely reported. In this work, we study the oxygen transfer and bubble size distributions in a real 100 L fermentation (producing poly(γ‐glutamic acid)) occurring under constant stirring speed (N) and under constant retrofitted power drawn (Pg/V). RESULTS For constant N fermentations, Pg/V and the volumetric mass transfer coefficient (kLa) decreased 40% and 70% during cultivation, respectively. Fermentations at constant Pg/V showed a decrease similar to that observed for constant N fermentations. Bubble size distributions were also similar for both operational conditions. Although an increase in bubble size was expected at constant N cultivations due to the increasing viscosity of the medium, the Sauter mean diameter (d32) decreased 65%. This singular behavior of d32 was likely caused by surfactin and bacillomycin, metabolites with strong surfactant activity produced by Bacillus velezensis 83 in the cultivations. The use of a constant Pg/V did not counteract the decrease in kLa due to the increase in viscosity. Although the presence of biosurfactants leads to an increase of interfacial transfer area by decreasing d32, the presence of these molecules and the increase in the medium viscosity affected kLa. CONCLUSIONS An atypical behavior of this system was found, especially in d32, different to that based on common correlations found in the literature. A complex interaction among the multiple factors involved in a real bioprocess for poly(γ‐glutamic acid) production was evidenced. © 2022 Society of Chemical Industry (SCI).

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Glucose limitation and glucose uptake rate determines metabolite production and sporulation in high cell density continuous cultures of Bacillus amyloliquefaciens 83

Cited by 12 publications

References 38 publications

Changes in the Carbon Metabolism of Escherichia coli During the Evolution of Doxycycline Resistance

Changes in the Carbon Metabolism of Escherichia coli During the Evolution of Doxycycline Resistance

Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion

Oxygen transfer and bubble sizes occurring in a pilot‐scale cultivation of Bacillus velezensis 83 for the production of poly(γ‐glutamic acid) under two schemes of power drawn

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