Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro

Petters, Robert M.; Johnson, Blake; Reed, Michael L.; Archibong, Anthony E.

doi:10.1530/jrf.0.0890269

Cited by 176 publications

(102 citation statements)

References 15 publications

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“…The beneficial effect of glutamine on embryonic development has been reported in hamsters [17], mice [4], pigs [22], and cattle [20]. In the present study, however, no beneficial effect of glutamine in combination with essential amino acids for basal medium Eagle (EAA-BME) and nonessential amino acids for minimum essential medium (NEAA-MEM) was observed even in the absence of glucose.…”

Section: Discussioncontrasting

confidence: 56%

Effects of Glutamine, Glycine and Taurine on the Development of In Vitro Fertilized Bovine Zygotes in a Chemically Defined Medium.

Takahashi

Kanagawa

1998

The Journal of Veterinary Medical Science

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ABSTRACT. The effects of glutamine, glycine and taurine on the development of bovine zygotes derived from IVM/IVF were determined using a protein-free chemically defined medium. After the cumulus cells were removed at 18 hr post-insemination, the presumptive zygotes were cultured for 4 or 6 days (about 104 or 154 hr) under a gas atmosphere of 5% O 2 : 5% CO 2 : 90% N 2 . A modified synthetic oviduct fluid medium supplemented with 20 amino acids (1 mM glutamine, essential amino acids for basal medium Eagle and nonessential amino acids for minimum essential medium), insulin, and PVA was used as a basic medium (mSOFai). Omitting 1 mM glutamine from mSOFai did not affect the embryonic development after 4 and 6 days of culture. After 4 days of culture, no significant effects of glycine and taurine on the development of zygotes to the morula stage were observed. However, supplementation with glycine or taurine significantly (P<0.05) affected, with no interaction, the embryonic development to blastocysts after 6 days of culture. Addition of 5 mM glycine and 2 or 10 mM taurine significantly (P<0.05) increased the percentage of blastocysts. The mean cell number in the blastocysts was affected by the glycine level, and was increased by the addition of 10 mM glycine (P<0.001). These results demonstrate that glycine and taurine in a chemically defined medium containing a group of essential and non-essential amino acids improve the development of bovine zygotes to the blastocyst stage under 5% O 2 . -KEY WORDS: bovine embryo, culture, glutamine, glycine, taurine.

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Section: Discussioncontrasting

confidence: 56%

Effects of Glutamine, Glycine and Taurine on the Development of In Vitro Fertilized Bovine Zygotes in a Chemically Defined Medium.

Takahashi

Kanagawa

1998

The Journal of Veterinary Medical Science

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“…Similar beneficial effects of glutamine on embryonic development have been reported in other species, including the hamster [18,25], rabbit [26], pig [27] and sheep [28]. The role of glutamine in embryonic development may be to act as a source of amine groups for transamination and/or as a source of energy, and a regulator of the detrimental effects of excessively high NaCl concentrations [16,22].…”

Section: Discussionsupporting

confidence: 54%

Optimisation of in vitro culture conditions in B6CBF1 mouse embryos

2004

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-This study was undertaken to investigate the effects of three media, volume and type of oil and frequency of observation on the in vitro development of mouse zygotes. B6CBF1 female mice (4 to 6 wk old) were superovulated using PMSG/hCG and mated with a proven fertile male of the same strain. Putative zygotes with polar bodies were collected from the oviducts of mated mice, 25-28 h after hCG injection, and were cultured in vitro. Embryo development was evaluated at either 96 h and 120 h or every 24 h for 120 h. The results obtained showed that the CZB medium was better than the KSOM and HCO 3 HTF media, and the use of 1 mL of paraffin oil was better than the use of 0.5 mL of paraffin oil. The effect of paraffin oil and mineral oil on embryo development was examined and the results indicated that the use of paraffin oil was better than the use of mineral oil. Repeated observations did not influence the proportion of embryos developing to blastocysts.in vitro development / media / type of oil / frequency of observation / mouse embryos

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“…The superiority of CZB to WM as a medium for mastomys embryo culture may be attributed to the presence of glutamine. Similar beneficial effects of glutamine on embryonic development were reported in hamster [15], pig [16], sheep [17], and a certain blocking mouse strain [7]. Glutamine is known to be used by preimplantation mammalian embryos as sources of energy or amino groups [18].…”

Section: Discussionsupporting

confidence: 54%

Preimplantation Embryo Development in Mastomys (Praomys coucha) In Vivo and In Vitro.

Ogura

Matsuda

Mochida

et al. 1997

Journal of Reproduction and Development

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Abstract. Mastomys (Praomys coucha) is a small murine rodent native in Africa. The present study was undertaken to investigate the developmental time course of mastomys embryos in vivo and the rate of development in vitro. Mastomys embryos developing in vivo exhibited a long 2-cell stage, which lasted from late Day 1 through Day 3. At Day 4, most embryos developed to the 8-cell or morula stages and at Day 5, all embryos reached the blastocyst stage. The attachment of embryos to the endometrium began at Day 5, as revealed by histological examinations. The development in vitro was investigated in different media with three inbred strains and an F1 hybrid. When 1-cell embryos from inbred mastomys were cultured in Whitten's medium (WM), none of them (n=61) developed beyond the 2-cell stage. Embryos of the same strains collected at Day 2 (2-cells) reached the blastocyst stage (46%, 31/67) in the same medium. Meanwhile, 1-cell embryos from F1 hybrid mastomys developed to the blastocyst stage in WM (13%, 13/71), indicating the presence of a strong heterosis effect. CZB medium, which lacks glucose and contains glutamine and EDTA, supported the development of 1-cell inbred embryos to the blastocyst stage (24%, 17/70). Supplementation of glutamine, but not EDTA, to WM stimulated the development of 1-cells to the blastocyst stage (22%, 11/51). These findings indicate that the development of mastomys embryos in vitro is both strain and medium dependent and that glutamine may play a crucial role in early development of mastomys embryos.

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Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro

Cited by 176 publications

References 15 publications

Effects of Glutamine, Glycine and Taurine on the Development of In Vitro Fertilized Bovine Zygotes in a Chemically Defined Medium.

Effects of Glutamine, Glycine and Taurine on the Development of In Vitro Fertilized Bovine Zygotes in a Chemically Defined Medium.

Optimisation of in vitro culture conditions in B6CBF1 mouse embryos

Preimplantation Embryo Development in Mastomys (Praomys coucha) In Vivo and In Vitro.

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