Optimisation of in vitro culture conditions in B6CBF1 mouse embryos

Zhu, Bi-ke; Walker, S.K.; Maddocks, S.

doi:10.1051/rnd:2004030

Cited by 12 publications

(10 citation statements)

References 37 publications

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“…The quality of the individual embryos was determined by a method described previously [28], scoring normal, abnormal or unfertilised. The definitions applied were:…”

Section: Morphological Evaluation Of Preimplantation Embryosmentioning

confidence: 99%

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Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse

Zhu

Setchell

2004

Reprod. Nutr. Dev.

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-The objective of this study was to examine the effect of paternal heat stress on the in vivo development of preimplantation embryos in the mouse. Synchronised B6CBF1 female mice were mated either to a control male mouse or to one that had been exposed at 7, 21 or 35 days previously, for 24 h to an ambient temperature of 36 ± 0.3 °C and 66 ± 5.6% relative humidity. Embryos were collected from the oviducts of mice at 14-16 h, 34-39 h or 61-65 h after mating or from the uterus at 85-90 h after mating and their developmental status was evaluated morphologically. The number of cells within blastocysts was also determined using bisbenzimide-propidium iodide staining. Paternal heat stress 7 days before mating reduced the proportion of embryos developing from 4-cell (4-C) to morulae (M), hatched blastocysts, total blastocysts and the number of inner cell mass (ICM) and trophectoderm (TE) cells in the blastocyst. Paternal heat stress 21 days prior to mating reduced the proportion of 2-C and 4-C to M embryos with no embryos developing to blastocysts. There were also increases in the number of 1-C and abnormal embryos recorded at this time. Paternal heat stress 35 days before mating decreased the proportion of 2-C embryos, expanded blastocysts and ICM and TE cells in the blastocyst. These results support previous work demonstrating that both the sperm in the epididymis and germ cells in the testis are susceptible to damage by environmental heat stress, with spermatocytes being the most vulnerable. This study also demonstrates that subtle effects on the male such as a short exposure to elevated environmental temperatures can translate to quite profound paternal impacts on early embryo development.paternal heat stress / in vivo development / mouse preimplantation embryos

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“…The quality of the individual embryos was determined by a method described previously [28], scoring normal, abnormal or unfertilised. The definitions applied were:…”

Section: Morphological Evaluation Of Preimplantation Embryosmentioning

confidence: 99%

“…The number of TE and ICM cells was determined by a method described previously [28]. Blastocysts were transferred from a Hepes-HTF medium [29] to acid Tyrode solution [30], under constant observation for 30-60 s, until the zonae pellucidae were completely dissolved.…”

Section: Differential Fluorescent Labelling Of Trophectoderm (Te) Andmentioning

confidence: 99%

Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse

Zhu

Setchell

2004

Reprod. Nutr. Dev.

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“…Cumulus cells were dispersed by a brief treatment with the Hepes HTF medium containing 300 μ g/mL of hyaluronidase type IV‐S (Sigma, Sydney, Australia). Control‐sired and heat‐sired putative zygotes were selected, washed three times, using the Hepes HTF medium, and finally cultured in 4‐well tissue culture dishes (Nunclon, Rador, PA, USA) with 50 μ L of a CZB medium (Zhu et al. , 2004) containing 5.6 m m glucose under paraffin oil (BDH, Melbourne, Australia) at 37 °C in an atmosphere of 5% CO 2 , 5% O 2 and 90% N 2 for later use.…”

Section: Methodsmentioning

confidence: 99%

The effect of paternal heat stress on protein profiles of pre‐implantation embryos in the mouse

Zhu¹,

Maddocks²

2005

Int J of Andrology

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The study was undertaken to compare the protein profiles of [35S]-methionine-labelled control-sired embryos with heat-sired embryos at 7, 14 or 21 days after mature fertile B6CBF F1 male mice were kept at 36 +/- 0.3 degrees C and 62 +/- 2.7% relative humidity for 24 h. One-dimensional gel electrophoresis and autoradiographs were used to examine the protein profiles between the two-cell embryos and the blastocysts. The results obtained demonstrate that paternal heat stress 7 or 14 days earlier did not apparently affect protein patterns of two-cell embryos, four-cell to eight-cell embryos, morulae or blastocysts. However, 21 days earlier, there were changes in protein patterns of two-cell embryos and abnormal embryos, but not the morulae. To further support and extend these results, two-dimensional gel electrophoresis and phosphorimaging were employed and the results obtained show that paternal heat stress 21 days before mating affected protein profiles of two-cell embryos and morulae in the mouse. Together, these findings have indicated that paternal heat stress affects most but not all protein patterns of pre-implantation embryos, which strongly supports our previous results demonstrating that paternal heat stress significantly reduced the developmental proportion of pre-implantation embryos in the mouse.

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“…Despite the indications that different types of commercial oil used for IVP can greatly influence embryonic development, few studies have directly compared their effectiveness in terms of IVP outcomes. In mice (Zhu, Walker, & Maddocks, ), bovine (Tae et al., ), human (Sifer et al., ) and porcine (Martinez, Nohalez, Parrilla et al., ) IVP systems, the use of oils known as PO increased the embryo development compared to the use of oils known as MO. These observations suggest that differences in oil composition might be associated with embryo IVP results.…”

Section: Does Oil Type Influence the Ivp Outcomes?mentioning

confidence: 99%

Importance of oil overlay for production of porcine embryos in vitro

Martínez

Gil

2017

Reprod Domestic Animals

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Technologies to edit the zygote genome have revolutionized biomedical research not only for the creation of animal models for the study of human disease but also for the generation of functional human cells and tissues through interspecies blastocyst complementation technology. The pig is the ideal species for these purposes due to its great similarity in anatomy and physiology to humans. Emerging biotechnologies require the use of oocytes and/or embryos of good quality, which might be obtained using in vitro production (IVP) techniques. However, the current porcine embryo IVP systems are still suboptimal and result in low monospermic fertilization and blastocyst formation rates and poor embryo quality. During recent years, intensive investigations have been performed to evaluate the influence of specific compounds on gametes and embryos and to avoid the use of undefined supplements (serum and serum derivate) in the incubation media. However, little consideration has been given to the use of the mineral oil (MO) to overlay incubation droplets, which, albeit being a routine component of the IVP systems, is a totally undefined and thus problematic product for the safety of gametes and embryos. In this review, we provide an overview on the advantages and disadvantages of using MO to cover the incubation media. We also review one important concern in IVP laboratories: the use of oils containing undetected contamination. Finally, we discuss the effects of different types of oils on the in vitro embryo production outcomes and the transfer of compounds from oil into the culture media.

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Optimisation of in vitro culture conditions in B6CBF1 mouse embryos

Cited by 12 publications

References 37 publications

Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse

Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse

The effect of paternal heat stress on protein profiles of pre‐implantation embryos in the mouse

Importance of oil overlay for production of porcine embryos in vitro

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