Glucose fermentation byClostridium butyricum grown under a self generated gas atmosphere in chemostat culture

Andel, J. van; Zoutberg, George R.; Crabbendam, Pia M.; Breure, A.M.

doi:10.1007/bf02660113

Cited by 102 publications

(39 citation statements)

References 18 publications

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“…2 h -~ but was more rapid at D = 0.2 h-1. Somewhat different results were obtained by Crabbendam et al (1985) and van Andel et al (1985), who reported no marked influence on selectivity of the glucose input concentration in glucose-limited cultures of C. butyricum.…”

Section: Influence Of the Glucose Input Concentrationmentioning

confidence: 69%

Butyrate production in continuous culture of Clostridium tyrobutyricum: effect of end-product inhibition

Michel-Savin

Marchal

Vandecasteele

1990

Appl Microbiol Biotechnol

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P r o d u c t i o n o f b u t y r a t e has b e e n studied in c o n t i n u o u s cultures o f Clostridium tyrobutyricum. Prod u c t i o n o f acids, gases a n d cell b i o m a s s were determ i n e d u n d e r c o n d i t i o n s o f glucose limitation by varying either the glucose i n p u t or the dilution rate. A d d ition of acetate or b u t y r a t e to the cultures was also b u t lower p r o d u c t c o n c e n t r a t i o n s o b t a i n e d in c o n t i n u o u s culture with respect to b a t c h or f e d -b a t c h conditions. It also e x p l a i n e d the decrease in the ratios o f b u t y r a t e to total acids a n d in cell yield observed at h i g h e r glucose i n p u t as well as the b e h a v i o u r o f the cultures u n d e r c o n d i t i o n s o f excess glucose. It could also p o s s i b l y a c c o u n t for the partial c o n v e r s i o n o f a d d e d acetate to b u t y r a t e o b s e r v e d at m o d e r a t e growth rates.

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Section: Influence Of the Glucose Input Concentrationmentioning

confidence: 69%

Butyrate production in continuous culture of Clostridium tyrobutyricum: effect of end-product inhibition

Michel-Savin

Marchal

Vandecasteele

1990

Appl Microbiol Biotechnol

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“…9,22 Nevertheless, in other cases, the final concentration of butyric acid could be around 10.0 g dm −3 or even significantly higher with no obvious inhibiting effects reported. van Andel et al 23 have reported cultivation of C butyricum strain LMD 77-11 on glucose in single-stage continuous fermentation in which the final But value was near 10 g dm −3 and notable growth and substrate uptake were observed. Strains E4 and E5 isolated from muds originating from a stagnant pond (the same isolation procedure and point with the strain under investigation F2b) have been reported to produce butyric acid at concentrations up to 16.0 g dm −3 in repeatedbatch fermentations of raw glycerol with no obvious inhibition reported.…”

Section: Continuous Culturesmentioning

confidence: 98%

The effect of raw glycerol concentration on the production of 1,3‐propanediol by Clostridium butyricum

Papanikolaou

Fick

Aggelis

2004

J of Chemical Tech & Biotech

101

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Raw glycerol, the main by-product of the bio-diesel production process, was converted to 1,3-propanediol by Clostridium butyricum F2b. In batch cultures, 47.1 g dm −3 of 1,3-propanediol were produced. Continuous cultures were conducted at a constant dilution rate (=0.04 h −1 ) and various inlet glycerol concentrations with 1,3-propanediol produced at levels up to 44.0 g dm −3 . At increasing glycerol concentrations in the inlet medium, biomass yield decreased. This decrease was attributed to the microbial metabolism being directed towards the biosynthesis of organic acids (and hence carbon losses as CO 2 ) instead of biochemical anabolic reactions. An autonomous analytical model was developed, and quantified the effect of inlet glycerol concentration on the production of biomass and metabolites. Indeed, high inlet substrate concentrations positively affected the biosynthesis, principally of butyric acid and to a lesser extent that of acetic acid. In contrast, at increased glycerol concentrations, the relative increase of 1,3-propanediol production per unit of substrate consumed was lower as compared with that of acetic and, mainly, butyric acid. This could be explained by the fact that the butyric acid pathway represents an alternative and competitive one to that of 1,3-propanediol for re-generation of NADH 2 equivalents in the microbial cell.

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“…To improve the economics of the fermentation process, it is desirable to increase butyrate production while reducing acetate production, which also reduces the product separation cost. Several factors influencing the selectivity for butyrate over acetate in the fermentation have been identified, including cell growth rate, glucose concentration or supply mode, and the partial pressure of H 2 (van Andel et al, 1985;Michel-Savin et al, 1990a, 1990b. Complete selectivity for butyrate production was shown to be possible in glucose-limited fed-batch cultures (Michel-Savin et al, 1990a, 1990b, but the reactor productivity and final product concentration were not high enough for economical production purpose.…”

Section: Introductionmentioning

confidence: 98%

Construction and characterization of pta gene‐deleted mutant of Clostridium tyrobutyricum for enhanced butyric acid fermentation

Zhu

Liu

Yang

2005

Biotech & Bioengineering

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Clostridium tyrobutyricum ATCC 25755 is an acidogenic bacterium, producing butyrate and acetate as its main fermentation products. In order to decrease acetate and increase butyrate production, integrational mutagenesis was used to disrupt the gene associated with the acetate formation pathway in C. tyrobutyricum. A nonreplicative integrational plasmid containing the phosphotransacetylase gene (pta) fragment cloned from C. tyrobutyricum by using degenerate primers and an erythromycin resistance cassette were constructed and introduced into C. tyrobutyricum by electroporation. Integration of the plasmid into the homologous region on the chromosome inactivated the target pta gene and produced the pta-deleted mutant (PTA-Em), which was confirmed by Southern hybridization. SDS-PAGE and two-dimensional protein electrophoresis results indicated that protein expression was changed in the mutant. Enzyme activity assays using the cell lysate showed that the activities of PTA and acetate kinase (AK) in the mutant were reduced by more than 60% for PTA and 80% for AK. The mutant grew more slowly in batch fermentation with glucose as the substrate but produced 15% more butyrate and 14% less acetate as compared to the wild-type strain. Its butyrate productivity was approximately 2-fold higher than the wild-type strain. Moreover, the mutant showed much higher tolerance to butyrate inhibition, and the final butyrate concentration was improved by 68%. However, inactivation of pta gene did not completely eliminate acetate production in the fermentation, suggesting the existence of other enzymes (or pathways) also leading to acetate formation. This is the first-reported genetic engineering study demonstrating the feasibility of using a gene-inactivation technique to manipulate the acetic acid formation pathway in C. tyrobutyricum in order to improve butyric acid production from glucose.

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Glucose fermentation byClostridium butyricum grown under a self generated gas atmosphere in chemostat culture

Cited by 102 publications

References 18 publications

Butyrate production in continuous culture of Clostridium tyrobutyricum: effect of end-product inhibition

Butyrate production in continuous culture of Clostridium tyrobutyricum: effect of end-product inhibition

The effect of raw glycerol concentration on the production of 1,3‐propanediol by Clostridium butyricum

Construction and characterization of pta gene‐deleted mutant of Clostridium tyrobutyricum for enhanced butyric acid fermentation

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