Glucose biosensor based on immobilization of glucose oxidase in electropolymerized poly(o-phenylenediamine) film on platinum nanoparticles-polyvinylferrocenium modified electrode

Turkmen, Esra; Baş, Salih Zeki; Gülce, Handan; Yıldız, Salih

doi:10.1016/j.electacta.2013.12.189

Cited by 91 publications

(49 citation statements)

References 60 publications

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“…Numerous studies (Talbert et al, 2014;Wong et al, 2008;Choi et al, 2015;Singh and Verma, 2013;Röcker et al, 2016) have shown increase in the traditional uses of this enzyme. In addition, GOx has more recently been used in biosensor applications (Devasenathipathy et al, 2015;Turkmen et al, 2014;Hwa, 2015;Lin et al, 2015) and other nanotechnological applications have been demonstrated Liu et al, 2014;Sapountzi et al, 2017). In this context, the new Amazonian source of this enzyme (A. niger LMM01) and production of GOx in a semi-continuous process demonstrates the importance of the present work.…”

Section: Discussionmentioning

confidence: 92%

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Sousa¹,

Santos²,

Cortez³

et al. 2017

Afr. J. Microbiol. Res.

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Glucose oxidase (GOx) has several industrial applications. It is believed that there are several species of fungi that have the ability to produce this enzyme, most of which are unexplored. This work aimed to investigate the production of glucose oxidase (EC 1.1.3.4) by fungi isolated from soil samples of the Amazonian forest. Filamentous fungi were isolated from soil samples from the Adolpho Ducke Forest Reserve, located in Manaus, Amazonas. Strains were subjected to submerged bioprocessing to select for the best GOx producers. Those selected for the production of isolated enzymes were subjected to kinetic tests that evaluated production of the enzyme and consumption of the biomass substrate by the isolates. In addition, experiments to evaluate the optimal carbon, nitrogen and phosphorus sources as well as the influence of the bioprocess factors were carried out. Finally, GOx production was investigated in a semi-continuous system for 7 days. The most frequent isolates isolated from soil samples belonged to the genera Aspergillus, Penicillium and Trichoderma. Aspergillus niger LMM01 was the best GOx producer. Glucose, peptone and KH 2 PO 4 were demonstrated to be the optimal carbon, nitrogen and phosphorus sources, respectively. Multivariate experiments demonstrated that the parameters with the greatest effect on GOx production were pH and agitation. Stable expression results for GOx (7.74 U/ml) were obtained over 7 days in a semi-continuous process. In this context, the new Amazonian source of this enzyme (A. niger LMM01), and enzyme production in a semi-continuous process, demonstrates the importance of the present work.

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Section: Discussionmentioning

confidence: 92%

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Sousa¹,

Santos²,

Cortez³

et al. 2017

Afr. J. Microbiol. Res.

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“…The amperometric results showed a signal related to electron transfer from H 2 O 2 as a byproduct of the enzymatic reaction. Furthermore, a signal enhancement was also obtained using a voltammetric measurement system with Pt NPs that was due to the electrocatalytic effects of these NPs [27]. …”

Section: The Functions Of Mnps In Various Biosensor Typesmentioning

confidence: 99%

“…Based upon their specific properties, NPs can act as immobilizing platforms [27–31], accelerate electron transfer [32, 33], catalyze the reaction of chemiluminecents with their substrates [34–37], amplify changes in mass [38, 39] and enhance refractive index (RI) changes [40, 41]. For instance, in addition to immobilizing the bioreceptors, MNPs can act as “electron wires” in electrochemical biosensors, which allow electrons produced in bioreactions to be transported to sensing electrodes or convert other physiochemical changes to measurable signals that are proportional to the analyte concentration [32].…”

Section: Introductionmentioning

confidence: 99%

Noble metal nanoparticles in biosensors: recent studies and applications

Malekzad

Zangabad

Mirshekari

et al. 2017

Nanotechnology Reviews

289

119

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The aim of this review is to cover advances in noble metal nanoparticle (MNP)-based biosensors and to outline the principles and main functions of MNPs in different classes of biosensors according to the transduction methods employed. The important biorecognition elements are enzymes, antibodies, aptamers, DNA sequences, and whole cells. The main readouts are electrochemical (amperometric and voltametric), optical (surface plasmon resonance, colorimetric, chemiluminescence, photoelectrochemical, etc.) and piezoelectric. MNPs have received attention for applications in biosensing due to their fascinating properties. These properties include a large surface area that enhances biorecognizers and receptor immobilization, good ability for reaction catalysis and electron transfer, and good biocompatibility. MNPs can be used alone and in combination with other classes of nanostructures. MNP-based sensors can lead to significant signal amplification, higher sensitivity, and great improvements in the detection and quantification of biomolecules and different ions. Some recent examples of biomolecular sensors using MNPs are given, and the effects of structure, shape, and other physical properties of noble MNPs and nanohybrids in biosensor performance are discussed.

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“…Os A presença das biomoléculas nos biossensores é que os tornam dispositivos altamente específicos e seletivos e a sua combinação com outros nanomateriais tem possibilitado a construção de biossensores para diferentes finalidades como, por exemplo, na área da saúde, são usados para monitorar a concentração de glicose, 21,22 colesterol, 23 ácido úrico, 24 2 Já na área ambiental são usados para detecção organofosforados 32 e metais pesados. 33 As principais vantagens deste tipo de dispositivo, em comparação com os métodos analíticos convencionais, estão no fato de serem pequenos, de fácil manipulação, relativamente baratos e realizam análise de forma rápida e específica.…”

Section: Biosensoresunclassified

Liposome-Encapsulated Biomolecules: Application in Enzymatic Biosensors and Immunosensors

Graça¹,

Ferreira²

2015

Revista Virtual De Química

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Abstract:Biomolecules are largely used in the development of biosensors since they present high specificity and selectivity with the analyte. However, these properties can be affected during and after immobilization on the transducer surface, impairing the good functioning of the device. However, the construction of a more accurate and stable device requires that these characteristics are retained after immobilization. In this case, biomolecules encapsulation in liposomes has helped to preserve the structure and activity of molecules immobilized on substrate. Therefore, this article shows the importance of combining the adsorption technique with the encapsulation of biomolecules in liposomes to improve the operation of these devices. Keywords:Biosensors; Biomolecules; Layer-by-Layer; Langmuir-Blodgett; liposome. ResumoAs biomoléculas são amplamente utilizadas no desenvolvimento de biossensores, pois apresentam alta especificidade e seletividade com o analito. No entanto, a construção de dispositivos mais precisos e estáveis requer que estas propriedades sejam mantidas após a imobilização, neste sentido a encapsulação de biomoléculas em lipossomos tem ajudado a preservar a estrutura e atividade das biomoléculas que são imobilizadas. Portanto, o presente artigo mostra a importância da combinação da técnica de adsorção com a encapsulação das biomoléculas em lipossomos para melhorar o funcionamento destes dispositivos.

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Glucose biosensor based on immobilization of glucose oxidase in electropolymerized poly(o-phenylenediamine) film on platinum nanoparticles-polyvinylferrocenium modified electrode

Cited by 91 publications

References 60 publications

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Noble metal nanoparticles in biosensors: recent studies and applications

Liposome-Encapsulated Biomolecules: Application in Enzymatic Biosensors and Immunosensors

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