Glucosamine Dose/Concentration-Effect Correlation in the Rat with Adjuvant Arthritis

Aghazadeh‐Habashi, Ali; Kohan, Hamed Gilzad; Asghar, Waheed; Jamali, Fakhreddin

doi:10.1002/jps.23819

Cited by 19 publications

(18 citation statements)

References 33 publications

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“…Glucosamine has already been reported to be effective in AIA rats . Following oral treatment, glucosamine is absorbed and metabolized, after which it may be incorporated into arthritic joints and support chondrocyte function .…”

Section: Discussionmentioning

confidence: 99%

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Protective effect of boiogito extract with glucosamine HCl against adjuvant‐induced arthritis in rats

Takenaga

Niimi

Hamaguchi

et al. 2018

Traditional & Kampo Medicine

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Aim: The effect of boiogito extract combined with glucosamine HCl was assessed in adjuvant-induced arthritis (AIA) rats. Methods: Rats received a daily oral mixture of boiogito extract (125 mg/kg) and glucosamine HCl (80 mg/kg) before and after once-off adjuvant injection. Treatment was continued up to the day when blood and/or tissue were collected (day 12 or day 21). Paw swelling, arthritis score, and inflammatory mediators were assessed. Results: Combined treatment was more effective for AIA than either agent alone. Both serum nitric oxide (NO) and tissue NO were significantly suppressed in the adjuvant-uninjected hind paw on day 12. Boiogito extract significantly reduced the tissue level of matrix metalloproteinase (MMP)-13. In addition, interleukin (IL)-1β-induced MMP-13 by SW1353 chondrocytes was dose-dependently suppressed by boiogito extract, while glucosamine HCl had little effect. IL-1β-induced phosphorylation of extracellular signal-related kinase (ERK) was inhibited by the combination of boiogito extract and glucosamine HCl, but was most strongly suppressed by glucosamine HCl alone. Conclusion: Combined treatment with boiogito extract and glucosamine HCl had a protective effect against AIA in rats. This effect was related to strong suppression of MMP-13 production (mainly by boiogito extract) and inhibition of inflammatory mediator production via ERK signaling (mainly by glucosamine HCl).

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Section: Discussionmentioning

confidence: 99%

“…Glucosamine has been shown to be effective for adjuvant arthritis in rats . In addition, Nakamura et al showed that glucosamine significantly improved pain and swelling of arthritic joints compared with placebo in patients with rheumatoid arthritis (RA) .…”

Section: Introductionmentioning

confidence: 99%

Protective effect of boiogito extract with glucosamine HCl against adjuvant‐induced arthritis in rats

Takenaga

Niimi

Hamaguchi

et al. 2018

Traditional & Kampo Medicine

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“…Considering the role of inflammation in OA, an anti-inflammatory agent should be beneficial in slowing down the progression of this disease [50,51]. Numerous animal [52][53][54][55] and human [56] studies reported that glucosamine possesses anti-inflammatory activities in OA. Aghazadeh-Habashi et al analyzed the effects of different doses of glucosamine (20,40,80, or 160 mg/kg/day) on rats with and found that 6-day glucosamine supplementation reduced the levels of proinflammatory cytokines IL-1, IL-6, and TNF-α and prevented the increase in the serum concentrations of nitrite, a stable metabolite of NO in rats with OA [52].…”

Section: Anti-inflammatory Activities Of Glucosaminementioning

confidence: 99%

“…Numerous animal [52][53][54][55] and human [56] studies reported that glucosamine possesses anti-inflammatory activities in OA. Aghazadeh-Habashi et al analyzed the effects of different doses of glucosamine (20,40,80, or 160 mg/kg/day) on rats with and found that 6-day glucosamine supplementation reduced the levels of proinflammatory cytokines IL-1, IL-6, and TNF-α and prevented the increase in the serum concentrations of nitrite, a stable metabolite of NO in rats with OA [52]. Li et al reported the effects of 15-day glucosamine treatment (40,80, and 160 mg/kg; oral) on mice with monosodium iodoacetate-induced OA and found that the mixture of glucosamine and chitooligosaccharides exerts strong anti-inflammatory effects by downregulating serum pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and C-reactive protein levels and upregulating the anti-inflammatory cytokine IL-2 level [53].…”

Section: Anti-inflammatory Activities Of Glucosaminementioning

confidence: 99%

“…Protected against cartilage degradation↓ levels of inflammatory proteins in the affected knee ↓ IL-1β and TNF-α [52] Rats received glucosamine for 16 days orally at doses of 20, 40, 80, or 160 mg/kg/day ↓ IL-6 and TNF levels ↑ serum nitrite [53] Mice with OA were treated for 15 days with glucosamine at doses of 40, 80, and 160 mg/kg/day ↓ joint swelling and OA symptoms ↓ IL-β, IL-6, TNF-α, and IL-2 Rats with OA were treated with 50 or 100 mg/kg glucosamine for 2 months ↑ cytokine IL-10 and ↓TGF-1 levels [55] Wistar rats were treated for 1 month with 500 mg of glucosamine sulfate/kg with chondroitin sulfate ↓ IL-1β in serum [56] Patients with temporomandibular joint OA treated for 1 month or 1 year with 40 and 80 mg of glucosamine with hyaluronic acid (vs placebo with hyaluronic acid)…”

Section: References Study Design Findingsmentioning

confidence: 99%

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Multifaceted Protective Role of Glucosamine against Osteoarthritis: Review of Its Molecular Mechanisms

et al. 2019

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Osteoarthritis (OA) is a joint disease resulting from cartilage degeneration and causing joint pain and stiffness. Glucosamine exerts chondroprotective effects and effectively reduces OA pain and stiffness. This review aims to summarise the mechanism of glucosamine in protecting joint health and preventing OA by conducting a literature search on original articles. Current evidence has revealed that glucosamine exhibits anti-inflammatory effects by reducing the levels of pro-inflammatory factors (such as tumour necrosis factor-alpha, interleukin-1, and interleukin-6) and enhancing the synthesis of proteoglycans that retard cartilage degradation and improve joint function. Additionally, glucosamine improves cellular redox status, reduces OA-mediated oxidative damages, scavenges free radicals, upregulates antioxidant proteins and enzyme levels, inhibits the production of reactive oxygen species, and induces autophagy to delay OA pathogenesis. In conclusion, glucosamine prevents OA and maintains joint health by reducing inflammation, improving the redox status, and inducing autophagy in joints. Further studies are warranted to determine the synergistic effect of glucosamine with other anti-inflammatory and/or antioxidative agents on joint health in humans.

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Cardiovascular effect of inflammation and nonsteroidal anti-inflammatory drugs on renin–angiotensin system in experimental arthritis

2017

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A co-morbidity of inflammatory conditions is increased cardio-renal risks. Additionally, nonsteroidal anti-inflammatory drugs (NSAIDs) which are used to treat pain and inflammation are also associated with increase in such risks. We hypothesized that inflammation and NSAIDs impose the cardio-renal risk through the activation of the renin-angiotensin-system (RAS), a regulating pathway of the renal and cardiovascular homeostasis. We investigated the effect of adjuvant arthritis and NSAIDs on the RAS. Western blotting and ELISA were used to measure the RAS components. Inflammation caused significant imbalances in the cardiac and renal angiotensin converting enzymes, their biologically active angiotensin peptides (AngII and Ang1-7) and the target proteins involved in the peptide-receptor binding (AngII type 1 and type 2, and Ang1-7 receptor, Mas) toward cardio-renal toxicity. However, 7 days treatment of arthritic animals with NSAIDs (rofecoxib, meloxicam, celecoxib and flurbiprofen) restored the constitutive balances, perhaps due to their anti-inflammatory properties. Inflammation exerts its cardio-renal effects by causing imbalance in the RAS. NSAIDs through their anti-inflammatory effect restore this imbalance. Thus, mechanisms other than imbalances in the RAS may be involved in the NSAIDs cardiotoxicity.

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Glucosamine Dose/Concentration-Effect Correlation in the Rat with Adjuvant Arthritis

Cited by 19 publications

References 33 publications

Protective effect of boiogito extract with glucosamine HCl against adjuvant‐induced arthritis in rats

Protective effect of boiogito extract with glucosamine HCl against adjuvant‐induced arthritis in rats

Multifaceted Protective Role of Glucosamine against Osteoarthritis: Review of Its Molecular Mechanisms

Cardiovascular effect of inflammation and nonsteroidal anti-inflammatory drugs on renin–angiotensin system in experimental arthritis

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