Glucocorticoid receptors in human fetal lung

Iacobelli, Stéfano; Ranelletti, F O; Sica, Gigliola; Barile, Giuseppe

doi:10.1007/bf03350382

Cited by 12 publications

(6 citation statements)

References 18 publications

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“…Hydrocortisone being a good competitor (Table 2), the dexamethasone binder could not be an aldosterone receptor. The sedimentation pattern is in the same order of magitude as found by other workers [4,7,11]. As mentioned above, Giannopoulos [7] was not able to detect a glucocorticoid receptor in adult human lung [4].…”

Section: Discussionsupporting

confidence: 71%

“…The apparent dissociation constant (5.8-21.0nM) is within the range of magnitude as those reported for other human glucocorticoid target tissues (adult liver: 1.7 nM [5], mammary carcinoma: 4.4-9.4 nM [20], malignant melanoma: 2-22 nM [4], fetal lung: 7.1 nM [11], A-549 cell line: 7.1 nM [12], human alveolar macrophages: 9.79 nM [17]. The glucocorticoid receptor content (mean+ S.D.…”

Section: Discussionmentioning

confidence: 73%

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Identification of glucocorticoid receptors in normal and neoplastic adult human lung

1983

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A dexamethasone-binding protein was detected in cytosol of five normal and three neoplastic lung tissue samples. The apparent dissociation constant of the dexamethasone protein complex ranged from 5.8 to 21 nM (11.4 +/- 5.7 nM for normal tissue cytosol, 12.1 +/- 7.7 nM for the neoplastic tissue cytosol). The binding protein sedimented at 8 S in sucrose density gradient at low ionic strength and showed a high specificity for glucocorticoids. It was concluded that the dexamethasone-binding protein had the characteristics of a glucocorticoid receptor. The glucocorticoid receptor content was determined to be 12-77 fmol/mg protein (37.1 +/- 18.9 fmol/mg protein for the normal tissue cytosol, 51.9 +/- 23.9 fmol/mg protein for the neoplastic tissue cytosol).

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 73%

Identification of glucocorticoid receptors in normal and neoplastic adult human lung

1983

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“…Ballard and Ballard reported that in the cytosol of lung tissue the GR concentration ranged from 40 to 350fmoYmg protein, in tissue obtained at autopsy from foetuses of 12-43 weeks of gestational age (3). Similar results (17) or lower GR concentrations (between 70 and 180 fmol/mg protein) (4) and between 1 1 0 and 300fmol/mg protein (5.1 were reported. The GR number in foetal lung tissue is independent of gestational age (3.…”

Section: Discussionmentioning

confidence: 71%

Glucocorticoid receptors in cord blood lymphocytes of healthy neonates and of preterms suffering from respiratory distress syndrome

et al. 1997

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We measured the number of glucocorticoid receptors (GR) in cord blood lymphocytes and the binding affinity (Kd) in 15 term and in 20 preterm babies. Thirteen preterms of the latter group received prenatal steroid treatment. Seven preterms developed neonatal respiratory distress syndrome (NRDS). The number of GR and the Kd were similar in the term and preterm (with and without NRDS) babies. The maximum (3H)-thymidine incorporation into DNA of cord blood lymphocytes from all preterms, with or without NRDS was suppressed when compared to that from term babies or adults. This could partly be explained by the antenatal steroid treatment. Sensitivity (ID50) of the lymphocytes for the inhibitory effect of dexamethasone was the same in all groups. In this study on the number and function of GR in lymphocytes, we were unable to find a relation between the functionality of the GR and the development of NRDS.

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“…Ozaki et a1.15 measured these receptors in bronchoalveolar cells obtained by lavage for the same indication of diffuse interstitial fibrosis. Receptor concentration was 5,581 2 3,741 sitedcells in cases of diffuse interstitial fibrosis and 9,970 & 4,052 sites/cells in healthy individuals. Bad responders to glucocorticoid treatment had the lowest receptor levels.…”

Section: Discussionmentioning

confidence: 93%

Glucocorticoid receptor concentrations in human lung at different growth stages

Labbé¹,

Grizard

Déchelotte

et al. 1990

Pediatric Pulmonology

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We measured glucocorticoid receptor concentrations in human lung at different stages of alveolar growth. Lung tissue was obtained from 9 surgically aborted or stillborn fetuses of 15 to 28 weeks gestational age, and from 6 infants and children, aged 2 months to 9 years, after lobar resection or at autopsy. Samples were taken from macroscopically healthy areas. Lung histology was performed in all cases. The receptor assay was done by establishing saturation curves for labeled dexamethasone in the absence or presence of a hundredfold excess of unlabeled dexamethasone. Total binding capacity and the dissociation constant were calculated from the saturation curves by the method of Scatchard. The receptor concentration in the fetuses was high (182 +/- 88 fmol/mg prot.), irrespective of gestational age. The lowest concentration (35 fmol/mg prot.) was found in a fetus with pulmonary hypoplasia. In the infants and children the mean receptor concentration was significantly lower (14.6 +/- 9.9 fmol/mg prot.); these included a case of sudden death in which the parenchymal structure was normal.

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Glucocorticoid receptors in human fetal lung

Cited by 12 publications

References 18 publications

Identification of glucocorticoid receptors in normal and neoplastic adult human lung

Identification of glucocorticoid receptors in normal and neoplastic adult human lung

Glucocorticoid receptors in cord blood lymphocytes of healthy neonates and of preterms suffering from respiratory distress syndrome

Glucocorticoid receptor concentrations in human lung at different growth stages

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