Gluco-obtusifolin and Its Aglycon, Obtusifolin, Attenuate Scopolamine-Induced Memory Impairment

Abstract

Abstract. In the present study, we assessed the effects of gluco-obtusifolin, isolated from the seeds of Cassia obtusifolia L., and its aglycone, obtusifolin, on the learning and memory impairments induced by scopolamine using the passive avoidance and the Morris water maze tasks in mice. Gluco-obtusifolin (1, 2, and 4 mg/ kg, p.o.) and obtusifolin (0.25, 0.5, 1, and 2 mg/kg, p.o.) significantly reversed scopolamine-induced cognitive impairments in the passive avoidance test (P<0.05). Moreover, gluco-obtusifolin (2 mg/kg, p.o.) and obtusifolin (0.5 mg/kg, p.o.) improved escape latencies, swimming times in the target quadrant, and crossing numbers in the zone where the platform previously existed in the Morris water maze test. In the acetylcholinesterase assay, gluco-obtusifolin and obtusifolin were found to inhibit acetylcholinesterase activity in vitro (IC 50 = 37.2 and 18.5 μM, respectively) and ex vivo. These results suggest that gluco-obtusifolin and its aglycone may be useful for the treatment of cognitive impairment, and that its beneficial effects are mediated, in part, by the enhancement of cholinergic signaling.

Anti-allodynic Effects of Obtusifolin and Gluco-Obtusifolin against Inflammatory and Neuropathic Pain

“…Obtusifolin and gluco-obtusifolin were dissolved in 10% Tween 80 solution, 4) and the dosage ranges of obtusifolin and gluco-obtusifolin treatment were based on a previous study. 4) Gabapentin, morphine, aspirin, and methotrexate were dissolved (or diluted) in physiological saline and administered intraperitoneally (i.p.)…”

“…4) Gabapentin, morphine, aspirin, and methotrexate were dissolved (or diluted) in physiological saline and administered intraperitoneally (i.p.) at a dose volume of 10 mL/kg for mice or 4 mL/kg for rats.…”

“…1) are anthraquinones found in the seeds of Cassia obtusifolia, which are widely used in traditional Chinese medicine. 4) Previous reports have shown that gluco-obtusifolin and obtusifolin have inhibitory effects on platelet aggregation and aldose reductase activity, respectively. 5) Another report showed that obtusifolin and gluco-obtusifolin ameliorate scopolamineinduced memory impairment in mice.…”

“…5) Another report showed that obtusifolin and gluco-obtusifolin ameliorate scopolamineinduced memory impairment in mice. 4) However, no reports have been published on the analgesic effects of obtusifolin and gluco-obtusifolin. Thus, the present study was performed to characterize the anti-allodynic effects of obtusifolin and gluco-obtusifolin in diverse models of pain induced by inflammatory (formalin and complete Freundʼs adjuvant (CFA) and neuropathic (chronic constriction nerve injury (CCI), lumbar 5 (L5) spinal nerve ligation (SNL), streptozotocin (STZ), and cancer chemotherapy factors.…”

Citidine-5-diphosphocholine Ameliorates the Impairment of Spatial Memory Induced by Scopolamine

“…: +81-92-871-6631; Fax: +81-92-863-0389; E-mail: takasa103@fukuoka-u.ac.jp water maze test in rats. 1,2) Scopolamine also causes learning and memory performance deficits in young human subjects in verbal tasks. 3) The memory impaired model induced by scopolamine is also called the amnesia model without neuronal injuries.…”