Glossiphoniid leeches as a touchstone for studies of development in clitellate annelids

Weisblat, David A.

doi:10.1016/bs.ctdb.2021.12.019

Cited by 2 publications

(1 citation statement)

References 90 publications

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“…1A, A’) are useful because their embryos are easily cultured in the laboratory and because the large, identifiable blastomeres in their early embryonic stages (Fig. 1B-1D and 1F) are amenable to a variety of experimental approaches, including intracellular injection (Kuo et al, 2020; Weisblat, 2022; Weisblat and Kuo, 2009; Weisblat and Kuo, 2014). On the other hand, the small size of its adult nervous system limits physiological approaches to studying its nervous system.…”

Section: Introductionmentioning

confidence: 99%

Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis

Kuo,

Szczupak,

Weisblat

et al. 2024

Preprint

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The analysis of how neural circuits function in individuals and change during evolution is simplified by the existence of neurons identified as homologous within and across species. Invertebrates, including leeches, have been used for these purposes in part because their nervous systems comprise a high proportion of identified neurons, but technical limitations make it challenging to assess the full extent to which assumptions of stereotypy hold true. Here, we introduce Minos plasmid-mediated transgenesis as a tool for introducing transgenes into the embryos of the leech Helobdella austinensis (Spiralia; Lophotrochozoa; Annelida; Clitellata; Hirudinida; Glossiphoniidae). We identified an enhancer driving pan-neuronal expression of markers, including histone2B:mCherry, which allowed us to enumerate neurons in segmental ganglia. Unexpectedly, we find that adult Helobdella ganglia contain fewer and more variable numbers of neurons than in previously examined leech species.

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Section: Introductionmentioning

confidence: 99%

Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis

Kuo,

Szczupak,

Weisblat

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis

Kuo,

Szczupak,

Weisblat

et al. 2024

Journal of Experimental Biology

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The analysis of how neural circuits function in individuals and change during evolution is simplified by the existence of neurons identified as homologous within and across species. Invertebrates, including leeches, have been used for these purposes in part because their nervous systems comprise a high proportion of identified neurons, but technical limitations make it challenging to assess the full extent to which assumptions of stereotypy hold true. Here, we introduce Minos plasmid-mediated transgenesis as a tool for introducing transgenes into the embryos of the leech Helobdella austinensis (Spiralia; Lophotrochozoa; Annelida; Clitellata; Hirudinida; Glossiphoniidae). We identified an enhancer driving pan-neuronal expression of markers, including histone2B:mCherry, which allowed us to enumerate neurons in segmental ganglia. Unexpectedly, we find that the segmental ganglia of adult transgenic Helobdella contain fewer and more variable numbers of neurons than in previously examined leech species.

show abstract

Glossiphoniid leeches as a touchstone for studies of development in clitellate annelids

Cited by 2 publications

References 90 publications

Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis

Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis

Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis

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