Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis
Dian-Han Kuo,
Lidia Szczupak,
David A. Weisblat
et al.
Abstract:The analysis of how neural circuits function in individuals and change during evolution is simplified by the existence of neurons identified as homologous within and across species. Invertebrates, including leeches, have been used for these purposes in part because their nervous systems comprise a high proportion of identified neurons, but technical limitations make it challenging to assess the full extent to which assumptions of stereotypy hold true. Here, we introduce Minos plasmid-mediated transgenesis as a… Show more
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