Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2015–2016

Gubareva, Larisa V.; Besselaar, Terry G.; Daniels, Rod S.; Fry, Alicia M.; Gregory, Vicki; Huang, Weijuan; Hurt, Aeron C.; Jorquera, Patricia A.; Lackenby, Angie; Leang, Sook-Kwan; Lo, Janice; Pereyaslov, Dmitriy; Rebelo-de-Andrade, Helena; Siqueira, Marilda Mendonça; Takashita, Emi; Odagiri, Takato; Wang, Dayang; Zhang, Wenqing; Meijer, Adam

doi:10.1016/j.antiviral.2017.08.004

Cited by 97 publications

(104 citation statements)

References 34 publications

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“…For A/H3N2 and B, no virus exhibiting reduced inhibition by NAIs was detected in Japan. These results were also similar to those reported by a global surveillance, which indicated that NAI‐resistant A/H3N2 and B viruses were detected at a lower frequency (<1.0%) compared with NAI‐resistant A/H1N1pdm09 viruses . This study showed that few AA mutations in the catalytic sites of A/H3N2 viruses were found without their D151G/N mutations.…”

Section: Discussionsupporting

confidence: 91%

“…Consequently, NA AA mutations associated with NAI resistance were only H275Y of H1N1pdm09. The frequency of H275Y detected in Japan was approximately 1%, which was similar to that reported by a global surveillance . For A/H3N2 and B, no virus exhibiting reduced inhibition by NAIs was detected in Japan.…”

Section: Discussionsupporting

confidence: 85%

“…Based on the reports on NA AA mutations associated with reduced inhibition by NAIs, RI/HRI‐related AA mutations were extracted from NA sequencing data examined in this study (Table ). Most (10/13 mutations) of the AA mutations detected in the catalytic sites of A/H3N2 viruses were D151G/N mutations, which were found to be induced by an MDCK cultivation .…”

Section: Resultsmentioning

confidence: 99%

“…The isolate exhibiting RI by oseltamivir in the 2015‐2016 season did not carry any of the RI‐related AA mutations (DS6‐349 in Table ). D199N in A/H1N1pdm09, D151E, K249E, G320E, and S331R in A/H3N2, and I262T in B were extracted as RI/HRI‐related AA mutations, based on their related references; however, no virus harboring these AA mutations showed substantial RI/HRI based on their IC 50 values.…”

Section: Resultsmentioning

confidence: 99%

“…A global surveillance system has been developed to detect NAI‐resistant influenza isolates and their related NA AA mutations . Currently, Japan is considered as the largest NAIs consuming country .…”

Section: Introductionmentioning

confidence: 99%

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Consecutive influenza surveillance of neuraminidase mutations and neuraminidase inhibitor resistance in Japan

Chong

Matsumoto

Kang

et al. 2019

Influenza Resp Viruses

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Background The large consumption of neuraminidase inhibitors ( NAI s) for the treatment of influenza virus infections places Japan at risk of becoming the epicenter of the global spread of NAI ‐resistant viruses. Objective To clarify NA amino acid mutations of epidemic influenza viruses in Japan and their related NAI resistance. Methods A total of 1791 samples, including 396 A/H1N1pdm09, 1117 A/H3N2, and 278 B isolates, were collected to determine of their 50% inhibitory concentration ( IC 50 ) values by NAI s (oseltamivir, zanamivir, peramivir, and laninamivir) during the Japanese seasons from 2011‐2012 to 2016‐2017. Then, 380 samples including 49 A/H1N1pdm09, 251 A/H3N2, and 80 B isolates were sequenced for the entire NA genes. Results Neuraminidase inhibitor‐resistant A/H1N1pdm09 viruses were detected at a frequency of 1.3% (5/396 isolates) in the epidemic seasons. None of the A/H3N2 and B viruses developed resistance to any of the four NAI s during the six seasons. Only five and 13 AA mutations were detected in the NA catalytic sites of A/H1N1pdm09 and A/H3N2 viruses, respectively. No mutations were observed in the catalytic sites of B viruses. Four of the five mutations in the catalytic sites of A/H1N1pdm09 consisted of H275Y, which was related to high resistance to oseltamivir and peramivir. Most (10/13) of the catalytic site mutations in A/H3N2 were associated with MDCK ‐passaged induction (D151G/N). Finally, no mutations related to substantial NAI resistance were detected in the A/H3N2 and B viruses examined. Conclusion These findings suggest that the NA catalytic sites of influenza viruses are well preserved. Even in Japan, no spread of NAI ‐resistant viruses has been observed, and A/H1N1pdm09 viruses carrying H275Y remain limited.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Consecutive influenza surveillance of neuraminidase mutations and neuraminidase inhibitor resistance in Japan

Chong

Matsumoto

Kang

et al. 2019

Influenza Resp Viruses

View full text Add to dashboard Cite

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Anti‐neuraminidase activity of chemical constituents of Balanophora involucrata

Sun

Zhang

Cao

et al. 2020

Biomedical Chromatography

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Balanophora involucrata J. D. Hooker has been known to possess potential anti‐inflammatory and antibacterial activities; however, its antiviral activity has not been evaluated so far. In order to find new neuraminidase inhibitors (NAIs), the neuraminidase (NA) inhibition activity of different B. involucrata extracts was evaluated. In this study, an in vitro NA inhibition assay was performed to identify which extract of B. involucrata exhibits (maximal) inhibitory activity against NA. Ultra high performance liquid chromatography/quadrupole time‐of‐flight–tandem mass spectroscopy (MS/MS) and molecular docking techniques were used to identify the specific compounds responsible for the anti‐influenza activity of the extract, and to explore the potential natural NAIs. The ethyl acetate extract of B. involucrata exhibited significant inhibitory activity against NA with 50% inhibitory concentration (IC50) value of 159.5 μg/mL. Twenty compounds were identified according to the MS/MS spectra; among them two compounds (quercitrin and phloridzin) showed obvious inhibitory activity against NA, with IC50 of 311.76 and 347.32 μmol/L, respectively. This study suggested that B. involucrata can be a potential natural source of NAIs and may be useful in the fight against ferocious influenza viruses.

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Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009

Güldemir

Coşkun-Ari

Altaş

et al. 2019

Journal of Medical Virology

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Influenza A(H1N1)pdm09 pandemic virus causing the 2009 global outbreak moved into the post‐pandemic period, but its variants continued to be the prevailing subtype in the 2015‐2016 influenza season in Europe and Asia. To determine the molecular characteristics of influenza A(H1N1)pdm09 isolates circulating during the 2015‐2016 season in Turkey, we identified mutations in the hemagglutinin (HA) genes and investigated the presence of H275Y alteration in the neuraminidase genes in the randomly selected isolates. The comparison of the HA nucleotide sequences revealed a very high homology (>99.5%) among the studied influenza A(H1N1)pdm09 isolates, while a relatively low homology (96.6%‐97.2%), was observed between Turkish isolates and the A/California/07/2009 vaccine virus. Overall 14 common mutations were detected in HA sequences of all 2015‐2016 influenza A(H1N1)pdm09 isolates with respect to the A/California/07/2009 virus, four of which located in three different antigenic sites. Eleven rare mutations in 12 HA sequences were also detected. Phylogenetic analysis revealed that all characterized influenza A(H1N1)pdm09 isolates formed a single genetic cluster, belonging to the genetic subclade 6B.1, defined by HA amino acid substitutions S84N, S162N, and I216T. Furthermore, all isolates showed an oseltamivir‐sensitive genotype, suggesting that Tamiflu (Oseltamivir) could still be the drug of choice in Turkey.

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Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2015–2016

Cited by 97 publications

References 34 publications

Consecutive influenza surveillance of neuraminidase mutations and neuraminidase inhibitor resistance in Japan

Consecutive influenza surveillance of neuraminidase mutations and neuraminidase inhibitor resistance in Japan

Anti‐neuraminidase activity of chemical constituents of Balanophora involucrata

Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009

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