Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009

Güldemir, Dilek; Coşkun-Ari, Fatma Filiz; Altaş, Ayşe Başak; Bakkaloğlu, Zekiye; Ünaldı, Özlem; Bayraktar, Fatma Sibel; Korukluoğlu, Gülay; Aktaş, Ali Rıza; Durmaz, Rıza

doi:10.1002/jmv.25565

Cited by 4 publications

(3 citation statements)

References 45 publications

(138 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The origin of this pattern could be related to the independent introduction of two genetically related FLUAV into pigs from different geographic sources during the international movement of animals and humans. It is probable that Colombian viruses from this cluster originated from a global human FLUAV, considering the presence of mutations K163G, E235D, and S74R, and the gain of an N-Glycosylation motif at 162 previously reported in pandemic viruses from humans [ 54 , 55 , 56 , 57 ]. The cluster likely reached swine populations in Eurasia and North America, from where it was then introduced to Colombia.…”

Section: Discussionmentioning

confidence: 94%

Sequence-Based Antigenic Analyses of H1 Swine Influenza A Viruses from Colombia (2008–2021) Reveals Temporal and Geographical Antigenic Variations

Ospina-Jimenez,

Gomez,

Rincon-Monroy

et al. 2023

Viruses

View full text Add to dashboard Cite

Swine influenza is a respiratory disease that affects the pork industry and is a public health threat. It is caused by type A influenza virus (FLUAV), which continuously undergoes genetic and antigenic variations. A large amount of information regarding FLUAV in pigs is available worldwide, but it is limited in Latin America. The HA sequences of H1 subtype FLUAV-positive samples obtained from pigs in Colombia between 2008–2021 were analyzed using sequence-based antigenic cartography and N-Glycosylation analyses. Of the 12 predicted global antigenic groups, Colombia contained five: four corresponding to pandemic strains and one to the classical swine H1N1 clade. Circulation of these clusters was observed in some regions during specific years. Ca2 was the immunodominant epitope among Colombian viruses. The counts of N-Glycosylation motifs were associated with the antigenic cluster ranging from three to five. The results show for the first time the existence of antigenic diversity of FLUAV in Colombia and highlight the impact of spatial and temporal factors on this diversity. This study provides information about FLUAV variability in pigs under natural conditions in the absence of vaccination and emphasizes the need for surveillance of its phylogenetic and antigenic characteristics.

show abstract

Section: Discussionmentioning

confidence: 94%

Sequence-Based Antigenic Analyses of H1 Swine Influenza A Viruses from Colombia (2008–2021) Reveals Temporal and Geographical Antigenic Variations

Ospina-Jimenez,

Gomez,

Rincon-Monroy

et al. 2023

Viruses

View full text Add to dashboard Cite

show abstract

“…Afterwards, the HA is cleaved by host proteases into HA1 and HA2 subunits. The HA1 subunit harbors the globular head domain containing five distinct antigenic epitopes (Sa, Sb, Ca1, Ca2, and Cb) and the sialic acid receptor-binding sites (RBSs) [10,11]; The HA2 subunit harbors the stalk domain which induces fusion between the viral envelope and host endosomal membrane [12]. Consequently, mutations within these domains might have potential effectiveness on the attachment of the virus to host cells and the recognition of the virus by the neutralizing antibodies aroused in human [13,14].…”

Section: Introductionmentioning

confidence: 99%

Whole-genome sequencing reveals origin and evolution of influenza A(H1N1)pdm09 viruses in Lincang, China, from 2014 to 2018

Zhao

Zhang

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

The continuous variation of the seasonal influenza viruses, particularly A(H1N1)pdm09, persistently threatens human life and health around the world. In local areas of southwest china, the large timescale genomic research on A(H1N1)pdm09 is still insufficient. Here, we sequenced 45 whole-genome sequences of influenza A(H1N1)pdm09 viruses in Lincang, China, from 2014 to 2018, by next-generation sequencing technology to characterize molecular mechanisms of their origin and evolution. Our phylogenetic analyses suggest that the A(H1N1)pdm09 strains circulating in Lincang belong to clade 6B and the subclade 6B.1A predominates in 2018. Further, the strains in 2018 possess elevated evolutionary rate as compared to strains in other years. Several newly emerged mutations for HA (hemagglutinin) in 2018 are revealed (i.e., S183P and R221K). Intriguingly, the substitution R221K falls into the RBS (receptor binding site) of HA protein, which could affect antigenic properties of influenza A(H1N1)pdm09 viruses, and another substitution S183P near to RBS with a high covering frequency (11/14 strains) in 2018 is exactly located at the epitope B. Notably, the NA (neuraminidase) protein harbors a new mutation I23T, potentially involved in N-glycosylation. Based on the background with a higher evolutionary rate in 2018 strains, we deeply evaluate the potential vaccine efficacy against Lincang strains and discover a substantive decline of the vaccine efficacy in 2018. Our analyses reaffirm that the real-time molecular surveillance and timely updated vaccine strains for prevention and control of influenza A(H1N1)pdm09 are crucial in the future.

show abstract

“…Influenza viruses know no boundaries, representing an example of rapid virus evolution combined with pressure exerted by the host’s immune system, enabling them to circulate between species and occasionally cross the interspecies barriers. Owing to the negative-stranded segmented RNA genome, influenza viruses constantly undergo spontaneous mutations resulting in small changes in their antigenic properties year to year ( Guldemir et al, 2019 ). These high mutation rates allow for the evasion of immunity.…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Molecular and Epidemiological Characterization of Influenza Viruses Circulating 2016–2020 in North Macedonia

et al. 2021

View full text Add to dashboard Cite

Influenza viruses know no boundaries, representing an example of rapid virus evolution combined with pressure exerted by the host’s immune system. Seasonal influenza causes 4–50 million symptomatic cases in the EU/EEA each year, with a global death toll reaching 650,000 deaths. That being the case, in 2014 North Macedonia introduced the sentinel surveillance in addition to the existing influenza surveillance in order to obtain more precise data on the burden of disease, circulating viruses and to implement timely preventive measures. The aims of this study were to give a comprehensive virological and epidemiological overview of four influenza seasons (2016–2020), assess the frequency and distribution of influenza circulating in North Macedonia and to carry out molecular and phylogenetic analyses of the hemagglutinin (HA) and neuraminidase (NA) genes of influenza A(H1N1)pdm09, A(H3N2) from ILI and SARI patients. Our results showed that out of 1,632 tested samples, 46.4% were influenza positive, with influenza A(H1N1)pdm09 accounting for the majority of cases (44%), followed by influenza B (32%) and A(H3N2) (17%). By comparing the sentinel surveillance system to the routine surveillance system, we showed that the newly applied system works efficiently and gives great results in the selection of cases. Statistically significant differences (p = < 0.0000001) were observed when comparing the number of reported ILI cases among patients aged 0–4, 5–14, 15–29, and 30–64 years to the reference age group. The phylogenetic analysis of the HA sequences unveiled the resemblance of mutations circulating seasonally worldwide, with a vast majority of circulating viruses belonging to subclade 6B.1A. The PROVEAN analysis showed that the D187A substitution in the receptor binding site (RBS) of the A(H1N1)pdm09 HA has a deleterious effect on the its function. The A(H3N2) viruses fell into the 3C.2a and 3C.3a throughout the analyzed seasons. Molecular characterization revealed that various substitutions in the A(H3N2) viruses gradually replaced the parental variant in subsequent seasons before becoming the dominant variant. With the introduction of sentinel surveillance, accompanied by the advances made in whole-genome sequencing and vaccine therapeutics, public health officials can now modify their approach in disease management and intervene effectively and in a timely manner to prevent major morbidity and mortality from influenza.

show abstract

Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009

Cited by 4 publications

References 45 publications

Sequence-Based Antigenic Analyses of H1 Swine Influenza A Viruses from Colombia (2008–2021) Reveals Temporal and Geographical Antigenic Variations

Sequence-Based Antigenic Analyses of H1 Swine Influenza A Viruses from Colombia (2008–2021) Reveals Temporal and Geographical Antigenic Variations

Whole-genome sequencing reveals origin and evolution of influenza A(H1N1)pdm09 viruses in Lincang, China, from 2014 to 2018

A Comprehensive Molecular and Epidemiological Characterization of Influenza Viruses Circulating 2016–2020 in North Macedonia

Contact Info

Product

Resources

About