Global Transcriptome Analysis ofStaphylococcus aureusResponse to Hydrogen Peroxide

Abstract

Staphylococcus aureus responds with protective strategies against phagocyte-derived reactive oxidants to infect humans. Herein, we report the transcriptome analysis of the cellular response of S. aureus to hydrogen peroxide-induced oxidative stress. The data indicate that the oxidative response includes the induction of genes involved in virulence, DNA repair, and notably, anaerobic metabolism.

Polymicrobial interactions stimulate resistance to host innate immunity through metabolite perception

“…1B). This is in stark contrast to similar studies with other bacteria, in which 140-520 genes were differentially expressed on H 2 O 2 exposure (18)(19)(20)(21)(22)(23) (18,20,23). More surprising was the H 2 O 2 -mediated induction of apiA.…”

“…Not surprisingly, A. actinomycetemcomitans displayed enhanced production of the H 2 O 2 -consuming enzyme catalase during coculture. This response has been observed previously in several bacterial species and serves to enhance resistance to H 2 O 2 (18)(19)(20)(21)(22)(23)29). More intriguing was the finding that A. actinomycetemcomitans also induces the outer membrane protein ApiA during coculture.…”

“…4 A and B). Although it is clear that katA is a critical component of H 2 O 2 resistance in numerous bacteria (18)(19)(20)(21)(22)(23)29), it was not known if the decrease in serum resistance was attributable to decreased levels of apiA. To test this possibility, apiA was constitutively expressed in the oxyR Ϫ mutant (27).…”

Hydrogen peroxide induced repression of icaADBC transcription and biofilm development in Staphylococcus epidermidis

“…10 Our data are largely consistent with a recent analysis of global changes in gene expression in S. aureus following 10-and 20-min exposures to 10 mM H 2 O 2 , which revealed an approximately three-fourfold reduction in icaADBC transcript levels. 19 Similarly, exposure to nitrite, which activates a number of oxidative stressresponsive genes, also inhibited biofilm formation in S. aureus by repressing expression of the ica gene cluster in an icaR-dependent manner. 20 Interestingly, in contrast to our findings in S. epidermidis, H 2 O 2 -mediated repression of the ica locus in S. aureus was not associated with changes in icaR transcription.…”

“…20 Interestingly, in contrast to our findings in S. epidermidis, H 2 O 2 -mediated repression of the ica locus in S. aureus was not associated with changes in icaR transcription. 19 Regulation of icaR expression and biofilm development in S. epidermidis appears to involve at least two regulatory pathways, the first of which is dependent on the alternative stress-responsive sigma factor s B , 21,22 and a second s B -independent signaling pathway triggered by ethanol. 10 In S. epidermidis, stress-induced activation of s B indirectly leads to repression of the icaR gene and activation of the icaADBC operon.…”