Global transcriptome analysis of Escherichia coli exposed to immobilized anthraquinone-2-sulfonate and azo dye under anaerobic conditions

Abstract

The immobilization of quinone compounds is regarded as a promising strategy to accelerate anaerobic decolorization of xenobiotic compounds azo dyes in the presence of quinone-reducing microorganisms. However, little is known about the basic response of these microorganisms to immobilized quinones in the presence of azo dyes. In the present study, whole-genome DNA microarrays were used to investigate a quinone-reducing bacterium Escherichia coli K-12 transcription response to immobilized anthraquinone-2-sulfonate (AQSim) reduction and azo dye acid red 18 (AR 18) decolorization. Transcriptome analysis showed that AQSim was more accessible for the cells of E. coli K-12 than AR 18. Despite there being some differences between AQSim and soluble AQS mediated decolorization of AR 18, AQSim reduction and AR 18 decolorization, more similarity could be observed in the four processes. Among over 60 % shared genes, several groups of genes exhibited high expression levels, including those genes encoding terminal reductases, menaquinone biosynthesis, formate dehydrogenases and outer membrane proteins. Especially, nrfABCD, frdBCD and dsmABC encoding terminal reductases were significantly upregulated. Further gene deletion experiments demonstrated that the above three groups of genes were involved in AQSim-mediated AR 18 decolorization. In addition, significant upregulation of stress response genes was observed, which indicated the adaptation of E. coli K-12 to AQSim and AR 18 exposures.

Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium

“…However, our GTA showed that in the SA-producing strain PB12.SA22, the galP gene was not significantly upregulated during EXP growth and the STA phase. This result was also observed in GTA for the parental strain PB12 in the same culture conditions (data not shown), suggesting that in both strains grown in complex fermentation broth for SA production, the OMP OmpC, LamB, and the maltose ABC transporter, have an important role in the transport of glucose because previous works reported the participation of these proteins in the uptake of glucose in E. coli [20-23]. All these genes were found to be upregulated in the EXP and STA1 phases, associated with the consumption of >30% of the initially added glucose to the fermentation broth; however, only in the STA1/STA2 comparison was detected the upregulation of ompC .…”

Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken

“…This was because many genes with iron and copper acquisition were up-regulated, which might have increased the concentration of copper in the E. coli 381 cytoplasm 47 , 87 . The repression of some genes encoding phage shock proteins may be due to different environmental stress conditions in E. coli , which fortified the resistant character of strain 88 .…”

Adaptive Responses of Shewanella decolorationis to Toxic Organic Extracellular Electron Acceptor Azo Dyes in Anaerobic Respiration

“…According to the data from Regprecise (http://regprecise.lbl.gov/), transcriptional factor NarP, regulated by RpoS, potentially regulated MtrCBA, MtrFED, OcmA, and OmpW, as well as a global regulator CRP (cyclic AMP receptor protein) in Shewanella spp., since the DNA binding sites where NarP activates their expressions were localized upstream of the coding regions. In addition, although azo dyes are toxic to S12, we did not find significant effects of the genes encoding transcriptional regulators of the MarR family, which determine the tolerance of E. coli to antibiotics, organic solvents, and oxidative stress, as well as azo dyes (62,73). It seems therefore that different regulative systems existed in S. decolorationis S12 and E. coli.…”