Global transcriptional responses of Acidithiobacillus ferrooxidans Wenelen under different sulfide minerals

Latorre, Mauricio; Ehrenfeld, Nicole; Cortés, María Paz; Travisany, Dante; Budinich, Marko; Aravena, Andrés; González, Maurício; Bobadilla-Fazzini, Roberto A.; Parada, Pilar; Maass, Alejandro

doi:10.1016/j.biortech.2015.09.110

Cited by 22 publications

(11 citation statements)

References 36 publications

(5 reference statements)

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“…Genome sequences for number of Acidithiobacillus spp. have become available in public databases since 2008, including the type strains of A. ferrooxidans, A. caldus, A. thiooxidans , and A. ferrivorans strain SS3 (Valdés et al, 2008, 2009, 2011; Liljeqvist et al, 2011; You et al, 2011; Talla et al, 2014; Travisany et al, 2014; Yin et al, 2014; Yan et al, 2015; Latorre et al, 2016; Zhang et al, 2016a). However, no genome, complete or draft, has yet been reported for A. ferridurans, A. ferriphilus and A. albertensis .…”

Section: Discussionmentioning

confidence: 99%

Molecular Systematics of the Genus Acidithiobacillus: Insights into the Phylogenetic Structure and Diversification of the Taxon

et al. 2017

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The acidithiobacilli are sulfur-oxidizing acidophilic bacteria that thrive in both natural and anthropogenic low pH environments. They contribute to processes that lead to the generation of acid rock drainage in several different geoclimatic contexts, and their properties have long been harnessed for the biotechnological processing of minerals. Presently, the genus is composed of seven validated species, described between 1922 and 2015: Acidithiobacillus thiooxidans, A. ferrooxidans, A. albertensis, A. caldus, A. ferrivorans, A. ferridurans, and A. ferriphilus. However, a large number of Acidithiobacillus strains and sequence clones have been obtained from a variety of ecological niches over the years, and many isolates are thought to vary in phenotypic properties and cognate genetic traits. Moreover, many isolates remain unclassified and several conflicting specific assignments muddle the picture from an evolutionary standpoint. Here we revise the phylogenetic relationships within this species complex and determine the phylogenetic species boundaries using three different typing approaches with varying degrees of resolution: 16S rRNA gene-based ribotyping, oligotyping, and multi-locus sequencing analysis (MLSA). To this end, the 580 16S rRNA gene sequences affiliated to the Acidithiobacillus spp. were collected from public and private databases and subjected to a comprehensive phylogenetic analysis. Oligotyping was used to profile high-entropy nucleotide positions and resolve meaningful differences between closely related strains at the 16S rRNA gene level. Due to its greater discriminatory power, MLSA was used as a proxy for genome-wide divergence in a smaller but representative set of strains. Results obtained indicate that there is still considerable unexplored diversity within this genus. At least six new lineages or phylotypes, supported by the different methods used herein, are evident within the Acidithiobacillus species complex. Although the diagnostic characteristics of these subgroups of strains are as yet unresolved, correlations to specific metadata hint to the mechanisms behind econiche-driven divergence of some of the species/phylotypes identified. The emerging phylogenetic structure for the genus outlined in this study can be used to guide isolate selection for future population genomics and evolutionary studies in this important acidophile model.

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Section: Discussionmentioning

confidence: 99%

Molecular Systematics of the Genus Acidithiobacillus: Insights into the Phylogenetic Structure and Diversification of the Taxon

et al. 2017

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“…Besides providing information regarding indigenous distinct iron‐oxidizing bacterial strains in the copper mine, this study led to the use of these indigenous bacteria for copper and zinc dissolution from the ore under optimized laboratory conditions. Indigenous microorganisms are more resistant toward the recalcitrant behavior of heavy metals present in leaching solutions after dissolution described by Latorre et al . The findings of bioleaching experiments revealed that these three isolates significantly leached copper and zinc.…”

Section: Discussionmentioning

confidence: 69%

“…Bioleaching of heavy metals has been amplified in recent years since of its applicability to low/lean grade ores and industrial effluents. One of the most important iron‐oxidizing bacterium using in bioleaching is Acidithiobacillus ferrooxidans , which is capable to oxidize both iron and sulfur moieties and use as an energy source . At.…”

Section: Introductionmentioning

confidence: 99%

Culture‐dependent hunt and characterization of iron‐oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction

Sajjad

Zheng

et al. 2018

J Basic Microbiol

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The exploration of microbial diversity in extremely acidic habitats has provided a vital base for the progression of minerals biotechnology. Three indigenous ironoxidizing acidophilic bacterial strains were isolated through serial dilution of enriched bacterial culture from Baiyin Copper Mine Stope, China. The morphological, biochemical, physiological, and phylogenetic characteristics of isolates were investigated. These isolates were motile, Gram-negative, and curved shape with pleomorphism except isolate WG101 that was a straight rod. The optimum growth pH and temperature for all isolates were 1.5 and 30°C, respectively, and showed extreme acidophilic nature. All the isolates showed obligate chemoautotrophic nature and used ferrous iron and pyrite as an energy source, however, isolates WG102 and WG103 were unable to use sulfur, while isolate WG101 could use elemental sulfur and reduced inorganic sodium thiosulfate as an energy source. The phylogenetic analyses based on 16S rRNA sequences revealed that the isolates WG101, WG102, and WG103 were homologous with Acidithiobacillus ferrooxidans strain AS2 (99%), Leptospirillum ferriphilum strain YSK (98%), and Leptospirillum ferrooxidans strain L15 (98%), respectively. These bacterial isolates showed efficient copper and zinc dissolution from the ore. The metals dissolution rate of At. ferrooxidans strain WG101 was 54.5 ± 4.33% (copper) and 49.6 ± 5% (zinc). The metals recovery rate of L.ferriphilum strain WG102 was 45.7 ± 3.5% (copper) and 40.5 ± 2.5% (zinc). The recovery rate of copper and zinc was 49.6 ± 4% and 46.5 ± 3% respectively in the case of L. ferrooxidans strain WG103. The findings of this study are consistent with the notion that the indigenous bacteria are more efficient in minerals dissolution. K E Y W O R D SAcidithiobacillus ferrooxidans, Baiyin Copper Mine, bioleaching, Leptospirillum ferriphilum, Leptospirillum ferrooxidansAbbreviations: AMD, acid mine drainage; EPS, exopolysaccharide; ORP, oxidation-reduction potential.

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“…The native A. ferrooxidans strain Wenelen (DSM 16786) isolated from an industrial copper biomining operation in Chile by Biosigma S.A. was recently reported to have an extreme capacity to resist high concentration of copper (over 10 g L −1 ) compared with the type strain ATCC 23270 (5 g L −1 ). Furthermore, the global transcriptional response of this native strain showed the upregulation of several genes related with copper tolerance when the bacterium was grown in the presence of pyrite and CuFeS 2 (Latorre et al ., ).…”

Section: Copper Resistance Of Biomining Bacteriamentioning

confidence: 97%

“…In the case of A. thiooxidans and A. caldus, as they oxidize only RISCs, an additional strain such as Leptospirillum ferrooxidans or a similar ferrous iron oxidizer would be required for suitable bioleaching of metal sulfides. Some biomining companies such as BioSigma, S.A. are currently using this approach and have isolated native species from copper mines such as A. ferrooxidans strain Wenelen (DSM 16786) and others with increased activity for copper leaching from mixed sulfide ores and high copper resistance (higher than 10 g L À1 compared with 5 g L À1 of the type A. ferrooxidans ATCC 23270 strain) for their use in industrial processes (Sugio et al, 2009;Latorre et al, 2016).…”

Section: Genetic Manipulation and Recent Advances Using Omics To Analmentioning

confidence: 99%

Microbial copper resistance: importance in biohydrometallurgy

Martínez-Bussenius

Navarro

Jerez

2016

Microbial Biotechnology

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SummaryIndustrial biomining has been extensively used for many years to recover valuable metals such as copper, gold, uranium and others. Furthermore, microorganisms involved in these processes can also be used to bioremediate places contaminated with acid and metals. These uses are possible due to the great metal resistance that these extreme acidophilic microorganisms possess. In this review, the most recent findings related to copper resistance mechanisms of bacteria and archaea related to biohydrometallurgy are described. The recent search for novel metal resistance determinants is not only of scientific interest but also of industrial importance, as reflected by the genomic sequencing of microorganisms present in mining operations and the search of those bacteria with extreme metal resistance to improve the extraction processes used by the biomining companies.

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Global transcriptional responses of Acidithiobacillus ferrooxidans Wenelen under different sulfide minerals

Cited by 22 publications

References 36 publications

Molecular Systematics of the Genus Acidithiobacillus: Insights into the Phylogenetic Structure and Diversification of the Taxon

Molecular Systematics of the Genus Acidithiobacillus: Insights into the Phylogenetic Structure and Diversification of the Taxon

Culture‐dependent hunt and characterization of iron‐oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction

Microbial copper resistance: importance in biohydrometallurgy

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