Global “omics” evaluation of human placental responses to preeclamptic conditions

Kedia, Komal; Smith, Stephen F.; Wright, Allison; Barnes, Justin M.; Tolley, H. Dennis; Esplin, M. Sean; Graves, Steven W.

doi:10.1016/j.ajog.2016.03.004

Cited by 9 publications

(5 citation statements)

References 45 publications

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“…The placental tissue is directly exposed to the uterine decidua, which is involved in maternal–foetal immune tolerance and pregnancy maintenance 6 . Although its exact pathogenesis and triggering conditions are still not fully clarified, it is widely believed that the placenta plays a central role in PE formation 7 . The most common cause of PE includes placental hypoxia, oxidative stress, disturbed angiogenesis, increased proinflammatory cytokines, complement dysregulation and endothelial dysfunction 5,8,9 .…”

Section: Introductionmentioning

confidence: 99%

“… 6 Although its exact pathogenesis and triggering conditions are still not fully clarified, it is widely believed that the placenta plays a central role in PE formation. 7 The most common cause of PE includes placental hypoxia, oxidative stress, disturbed angiogenesis, increased proinflammatory cytokines, complement dysregulation and endothelial dysfunction. 5 , 8 , 9 Recent research has found that the immune system and mitochondrial dysfunction play an essential role in the pathophysiology of PE.…”

Section: Introductionmentioning

confidence: 99%

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Systematic proteomics analysis of lysine acetylation reveals critical features of placental proteins in pregnant women with preeclampsia

Shangguan

Wang

Shi

et al. 2021

J Cellular Molecular Medi

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Preeclampsia (PE) is a dangerous hypertensive disorder that occurs during pregnancy. The specific aetiology and pathogenesis of PE have yet to be clarified. To better reveal the specific pathogenesis of PE, we characterized the proteome and acetyl proteome (acetylome) profile of placental tissue from PE and normal‐term pregnancy by label‐free quantification proteomics technology and PRM analysis. In this research, 373 differentially expressed proteins (DEPs) were identified by proteome analysis. Functional enrichment analysis revealed significant enrichment of DEPs related to angiogenesis and the immune system. COL12A1, C4BPA and F13A1 may be potential biomarkers for PE diagnosis and new therapeutic targets. Additionally, 700 Kac sites were identified on 585 differentially acetylated proteins (DAPs) by acetylome analyses. These DAPs may participate in the occurrence and development of PE by affecting the complement and coagulation cascades pathway, which may have important implications for better understand the pathogenesis of PE. In conclusion, this study systematically analysed the reveals critical features of placental proteins in pregnant women with PE, providing a resource for exploring the contribution of lysine acetylation modification to PE.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systematic proteomics analysis of lysine acetylation reveals critical features of placental proteins in pregnant women with preeclampsia

Shangguan

Wang

Shi

et al. 2021

J Cellular Molecular Medi

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show abstract

“…While, Pe leads to the morbidity and mortality of mothers and perinatal infants, its pathogenesis has not been fully elucidated (1,3). The placenta is crucial for the development of Pe (4). it has been shown that placental hypoxia is considered to be the main factor contributing to the pathogenesis of Pe, and is associated with excessive apoptosis of trophoblasts, which results in decreased trophoblast invasion and insufficient spiral artery remodeling (5,6).…”

Section: Introductionmentioning

confidence: 99%

HIF‑1α affects trophoblastic apoptosis involved in the onset of preeclampsia by regulating FOXO3a under hypoxic conditions

et al. 2020

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Preeclampsia (Pe) is a pregnancy-specific syndrome that has severe implications on perinatal mortality and morbidity. excessive apoptosis of trophoblasts induced by hypoxia may be associated with the development of Pe, but the exact pathogenesis is unknown. Forkhead box o transcription factor 3a (FoXo3a) is activated under hypoxic conditions. Furthermore, hypoxia-inducible factor-1α (HiF-1α) is sensitive to variations in partial oxygen pressure. Thus, the aims of the present study were to investigate the expression levels of HiF-1α and FoXo3a in placental samples of early onset severe Pe, and their effect on trophoblastic apoptosis under hypoxic conditions. cobalt chloride was used to establish the hypoxic model. The present study examined the expression levels of HiF-1α and FoXo3a in the placental tissues and HTr8/SVneo cells under hypoxic conditions. it was found that HiF-1α and FoXo3a were highly expressed in placental tissues of patients with Pe and in HTr8/SVneo cells under hypoxic conditions. Furthermore, knockdown of FOXO3a using a specific small interfering rna (sirna) decreased apoptosis in HTr8/SVneo cells. Moreover, it was found that after knockdown of HiF-1α using sirna, FoXo3a expression and the apoptotic rate were reduced in HTr8/SVneo cells. Therefore, the present results indicated that the elevated expression of HiF-1α increased trophoblastic apoptosis by regulating FoXo3a, which may be involved in the pathogenesis of Pe.

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“…During the last decades, there have been many substantial advances in the pathophysiology of preeclampsia. The placenta plays an important role in the development of preeclampsia (Kedia 2016). It has been demonstrated that placental hypoxia is considered a major factor contributing to the pathogenesis of preeclampsia, and is associated with excessive trophoblast apoptosis, resulting in decreased trophoblast invasion and insufficient remodeling of the spiral arteries (Zhang et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Hypoxia Inducible Factor-1-Alpha Expression on Preeclampsia Mice Model With L-Arginine Administration

Anggraini

Sulistyowati

Bachnas

et al. 2021

FMI

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Preeclampsia is hypertension in pregnancy that aﬀects 2% to 8% of pregnancies worldwide and causes significant maternal and perinatal morbidity and mortality. In the pathogenesis of preeclampsia, placental hypoxia plays an important role, associated with excessive trophoblast apoptosis resulting in decreased trophoblast and spiral arteries invasion. This placental hypoxic condition will induce increased expression of Hypoxia Inducible Factor -1-Alpha (HIF-1-A). L-Arginine is a potent vasodilator presumably to improve preeclampsia placental hypoxic conditions and reduce HIF-1-A expression. This study was an experimental study with a parallel-group post-test only design. Thirty-six preeclamptic mice models were divided into 2 groups. The control group (K1) 18 preeclamptic mice model without treatment and the treatment group (K2) 18 preeclamptic mice given L-Arginine. The independent variable was the administration of L-Arginine and the dependent variable is the placental HIF-1-A expression. Statistical analysis used unpaired t-test on normal data distribution, and Mann Whitney test on abnormal data distribution. The mean of placental HIF-1-A expression K1 was 2.47 ± 1.65 with a minimum value of 0.4 and a maximum value of 6.6. At K2 0.93 ± 0.55 with a minimum value of 0.0 and a maximum value of 2.0. Statistical tests showed that the placental HIF-1-A expression in the treatment group was significantly lower than that in the control group (p <0.001). In conclusion, the expression of HIF-1-A in preeclamptic mice model placenta decreased with L-Arginine administration.

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Global “omics” evaluation of human placental responses to preeclamptic conditions

Cited by 9 publications

References 45 publications

Systematic proteomics analysis of lysine acetylation reveals critical features of placental proteins in pregnant women with preeclampsia

Systematic proteomics analysis of lysine acetylation reveals critical features of placental proteins in pregnant women with preeclampsia

HIF‑1α affects trophoblastic apoptosis involved in the onset of preeclampsia by regulating FOXO3a under hypoxic conditions

Hypoxia Inducible Factor-1-Alpha Expression on Preeclampsia Mice Model With L-Arginine Administration

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