Global insights into acetic acid resistance mechanisms and genetic stability of Acetobacter pasteurianus strains by comparative genomics

Abstract

Acetobacter pasteurianus (Ap) CICC 20001 and CGMCC 1.41 are two acetic acid bacteria strains that, because of their strong abilities to produce and tolerate high concentrations of acetic acid, have been widely used to brew vinegar in China. To globally understand the fermentation characteristics, acid-tolerant mechanisms and genetic stabilities, their genomes were sequenced. Genomic comparisons with 9 other sequenced Ap strains revealed that their chromosomes were evolutionarily conserved, whereas the plasmids were unique compared with other Ap strains. Analysis of the acid-tolerant metabolic pathway at the genomic level indicated that the metabolism of some amino acids and the known mechanisms of acetic acid tolerance, might collaboratively contribute to acetic acid resistance in Ap strains. The balance of instability factors and stability factors in the genomes of Ap CICC 20001 and CGMCC 1.41 strains might be the basis for their genetic stability, consistent with their stable industrial performances. These observations provide important insights into the acid resistance mechanism and the genetic stability of Ap strains and lay a foundation for future genetic manipulation and engineering of these two strains.

Complete Genome Sequences of Two Acetic Acid-Producing Acetobacter pasteurianus Strains (Subsp. ascendens LMG 1590T and Subsp. paradoxus LMG 1591T)

“…The results showed that A. pasteurianus NBRC 101655, A. pasteurianus 386B, and eight A. pasteurianus IFO strains were quite similar with respect to genome structure in the chromosome (Figure 1), which was inconsistent with previous reports (Illeghems et al, 2013; Wang et al, 2015). Moreover, rearrangements, deletions, amplifications, and insertions occurred frequently in A. pasteurianus Ab3, A. pasteurianus subsp.…”

A meta-proteomics approach to study the interspecies interactions affecting microbial biofilm development in a model community

“…Arginine decarboxylases have previously been associated with maintenance of bacterial pH homeostasis during acid stress 33 , 34 , which would likely occur during fermentative metabolism. Similarly, both spermidine and putrescine have been associated with acid stress 35 , 36 . A schematic overview of spermine formation by the arginine metabolism, with the related shifts in protein abundance from Xanthomonas , is presented in Supplementary Fig.…”

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

“…Moreover, the K. hansenii clade again showed to be clearly separated from all the rest of the analyzed strains with the smallest number of toxin–antitoxin systems and unique presence of CRISPR‐Cas system. The importance of the CRISPR‐Cas system in maintaining genome stability in other AAB representatives, Acetobacter pasteurianus CICC 20001 and CGMCC 1.41 strains and their derivatives, was proved recently (Wang, Shao, Chen, Chen, & Chen, ), but no such studies have been done for the Komagataeibacter genus so far.…”