Global gene expression profiles of canine macrophages and canine mammary cancer cells grown as a co-culture in vitro

Król, Magdalena; Pawłowski, Karol; Majchrzak, Kinga; Gajewska, Małgorzata; Majewska, Alicja; Motyl, T.

doi:10.1186/1746-6148-8-16

Cited by 28 publications

(58 citation statements)

References 49 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly to human breast cancer, in canine mammary cancer the macrophages also polarize toward the M2 phenotype [57]. Recently, we and others demonstrated that TAM infiltration is significantly associated with VEGF expression in malignant canine mammary tumors and that genes involved in angiogenic cellular pathways have been significantly upregulated in macrophages cocultured with canine mammary tumor cell lines [20, 58]. …”

Section: Inflammatory Cells and Sustained Angiogenesismentioning

confidence: 99%

“…There are few studies in canine mammary cancer regarding the influence of immune cells in tumor invasion and metastasis [55, 64, 95]. The role of lymphocytes and macrophages in canine mammary tumor metastasis is not fully understood.…”

Section: Inflammatory Cells Tissue Invasion and Metastasismentioning

confidence: 99%

“…In terms of TAMs, their density was significantly higher in canine mammary adenocarcinomas that metastasized [64]. Furthermore, a microarray analysis to determine the global gene expression of a coculture of canine macrophages and canine mammary cancer cells showed an upregulation of genes involved in angiogenesis in TAMs and an increase in the migratory and invasive capabilities of cancer cells [95]. Mucha et al showed, for the first time, that MDSCs demonstrated increased activation of IL-28/IL-28RA (IFN- γ ) signaling, which stimulates STAT3 in canine mammary tumor cells therefore promoting epithelial-mesenchymal transition (EMT) and increased invasion and migration [55].…”

Section: Inflammatory Cells Tissue Invasion and Metastasismentioning

confidence: 99%

See 2 more Smart Citations

A Comparative Approach of Tumor-Associated Inflammation in Mammary Cancer between Humans and Dogs

Carvalho

Silva-Carvalho

Pires

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

Infiltrating cells of the immune system are widely accepted to be generic constituents of tumor microenvironment. It has been well established that the development of mammary cancer, both in humans and in dogs, is associated with alterations in numbers and functions of immune cells at the sites of tumor progression. These tumor infiltrating immune cells seem to exhibit exclusive phenotypic and functional characteristics and mammary cancer cells can take advantage of signaling molecules released by them. Cancer related inflammation has an important role in mammary carcinogenesis, contributing to the acquisition of core hallmark capabilities that allow cancer cells to survive, proliferate, and disseminate. Indeed, recent studies in human breast cancer and in canine mammary tumors have identified a growing list of signaling molecules released by inflammatory cells that serve as effectors of their tumor-promoting actions. These include the COX-2, the tumor EGF, the angiogenic VEGF, other proangiogenic factors, and a large variety of chemokines and cytokines that amplify the inflammatory state. This review describes the intertwined signaling pathways shared by T-lymphocytic/macrophage infiltrates and important tissue biomarkers in both human and dog mammary carcinogenesis.

show abstract

Section: Inflammatory Cells and Sustained Angiogenesismentioning

confidence: 99%

Section: Inflammatory Cells Tissue Invasion and Metastasismentioning

confidence: 99%

Section: Inflammatory Cells Tissue Invasion and Metastasismentioning

confidence: 99%

See 1 more Smart Citation

A Comparative Approach of Tumor-Associated Inflammation in Mammary Cancer between Humans and Dogs

Carvalho

Silva-Carvalho

Pires

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…The optimal annealing time was 5 sec, whereas optimal temperature was 61°C. rps19 and hprt genes were used as a non-regulated reference for the normalization of target gene expression (sequences and reaction conditions has been previously given an account of: [23-25,28,29]. …”

Section: Methodsmentioning

confidence: 99%

A role of ghrelin in canine mammary carcinoma cells proliferation, apoptosis and migration

et al. 2012

Self Cite

View full text Add to dashboard Cite

BackgroundGhrelin is a natural ligand of the growth hormone secretagogue receptor (GHS-R). They are often co-expressed in multiple human tumors and related cancer cell lines what can indicate that the ghrelin/GHS-R axis may have an important role in tumor growth and progression. However, a role of ghrelin in canine tumors remains unknown. Thus, the aim of our study was two-fold: (1) to assess expression of ghrelin and its receptor in canine mammary cancer and (2) to examine the effect of ghrelin on carcinoma cells proliferation, apoptosis, migration and invasion. The expression of ghrelin and its receptor in canine mammary cancer tissues and cell lines (isolated from primary tumors and their metastases) was examined using Real-time qPCR and immunohistochemistry. For apoptosis analysis the Annexin V and propidium iodide dual staining was applied whereas cell proliferation was evaluated by MTT assay and BrdU incorporation test. The influence of ghrelin on cancer cells migration and invasion was assessed using Boyden chamber assays and wound healing assay.ResultsThe highest expression of ghrelin was observed in metastatic cancers whereas the lowest expression of ghrelin receptor was detected in tumors of the 3rd grade of malignancy. Higher expression of ghrelin and its receptor was detected in cancer cell lines isolated from metastases than in cell lines isolated from primary tumors. In vitro experiments demonstrated that exposure to low doses of ghrelin stimulates cellular proliferation, inhibits apoptosis and promotes motility and invasion of canine mammary cancer cells. Growth hormone secretagogue receptor inhibitor ([D-Lys3]-GHRP6) as well as RNA interference enhances early apoptosis.ConclusionThe presence of ghrelin and GHS-R in all of the examined canine mammary tumors may indicate their biological role in cancer growth and development. Our experiments conducted in vitro confirmed that ghrelin promotes cancer development and metastasis.

show abstract

“…Activation of CSF-1R by its ligand is likely to occur in an autocrine manner in tumor cells in which CSF-1R and CSF-1 are co-expressed, or in paracrine manner, when CSF-1R is stimulated by CSF-1 released by fibroblasts [6]. However, our recent studies have shown that CSF-1R in cancer cells can be stimulated by colony stimulating factor secreted by tumor-associated macrophages [8]. Interestingly, we showed that their co-culture leads to up-regulation of CSF in macrophages and up-regulation of CSFs and CSF-1R in cancer cells.…”

Section: Introductionmentioning

confidence: 99%

CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells

et al. 2013

Self Cite

View full text Add to dashboard Cite

BackgroundTumor-associated macrophages (TAMs) have high impact on the cancer development because they can facilitate matrix invasion, angiogenesis, and tumor cell motility. It gives cancer cells the capacity to invade normal tissues and metastasize. The signaling of colony-stimulating factor-1 receptor (CSF-1R) which is an important regulator of proliferation and differentiation of monocytes and macrophages regulates most of the tissue macrophages. However, CSF-1R is expressed also in breast epithelial tissue during some physiological stages i.g.: pregnancy and lactation. Its expression has been also detected in various cancers. Our previous study has showed the expression of CSF-1R in all examined canine mammary tumors. Moreover, it strongly correlated with grade of malignancy and ability to metastasis. This study was therefore designed to characterize the role of CSF-1R in canine mammary cancer cells proliferation, apoptosis, migration, and invasion. As far as we know, the study presented hereby is a pioneering experiment in this field of veterinary medicine.ResultsWe showed that csf-1r silencing significantly increased apoptosis (Annexin V test), decreased proliferation (measured as Ki67 expression) and decreased migration (“wound healing” assay) of canine mammary cancer cells. Treatment of these cells with CSF-1 caused opposite effect. Moreover, csf-1r knock-down changed growth characteristics of highly invasive cell lines on Matrigel matrix, and significantly decreased the ability of these cells to invade matrix. CSF-1 treatment increased invasion of cancer cells.ConclusionThe evidence of the expression and functional role of the CSF-1R in canine mammary cancer cells indicate that CSF-1R targeting may be a good therapeutic approach.

show abstract

Global gene expression profiles of canine macrophages and canine mammary cancer cells grown as a co-culture in vitro

Cited by 28 publications

References 49 publications

A Comparative Approach of Tumor-Associated Inflammation in Mammary Cancer between Humans and Dogs

A Comparative Approach of Tumor-Associated Inflammation in Mammary Cancer between Humans and Dogs

A role of ghrelin in canine mammary carcinoma cells proliferation, apoptosis and migration

CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells

Contact Info

Product

Resources

About