Global expression profiling of metabolic pathway‐related lncRNAs in human gastric cancer and the identification of RP11‐555H23.1 as a new diagnostic biomarker

Background Accumulated evidences indicate that long non‐coding RNAs (lncRNAs) participate in many biological mechanisms. Moreover, it acts as an essential regulator in various human diseases such as gastric cancer (GC). Nevertheless, the comprehensive regulatory roles and clinical significance of most lncRNAs in GC are not fully understood. Methods In this research, our aim was to investigate the underlying mechanism of lncRNA LINC01234 in GC. Firstly, the usage of qRT‐PCR helped to establish expression pattern of LINC01234 in GC tissues. Following this, appropriate statistical tests were applied to analyze the relation between expression level and clinicopathological factors. Ultimately, potential functions and regulatory network of LINC01234 were concluded via GSEA and a series of bioinformatics tools or databases, respectively. Results Consequently, at the end of research we found LINC01234 is up‐regulated in GC tissues in comparison with adjacent normal tissues. Furthermore, its expression level is correlated with differentiation of patients with GC. It is also important to highlight bioinformatics analysis revealed that LINC01234 is involved in cancer‐associated pathways such as cell cycle and mismatch repair. Also, regulatory network of LINC01234 presented a probability in the involvement of tumorigenesis through regulating cancer‐associated genes. Conclusion Overall, our results suggested that LINC01234 may play a crucial role in GC.

Section: Discussionmentioning

confidence: 99%

Function and regulation annotation of up‐regulated long non‐coding RNA LINC01234 in gastric cancer

Zhu

Luo

Korakkandan

et al. 2020

“…The detection of piRNA-1245 in GJ could be a new avenue for differentiating GC from healthy people. [24][25][26][27][28][29] Some altered ncRNAs played a crucial role in the regulation of malignant phenotypes of tumor. 24 Mo et al elucidated that lncRNA RP11-555H23.1 showed an important role in the occurrence of GC, and used as a new marker of GC.…”

Section: Accumulating Literatures Showed That Alternation Of Non-codingmentioning

confidence: 99%

“…24 Mo et al elucidated that lncRNA RP11-555H23.1 showed an important role in the occurrence of GC, and used as a new marker of GC. 26 Zhu et al 29 identified that tiRNA-5034-GluTTC-2 might be responsible for a marker for GC diagnosis. Tan et al established that detection of miR-145 and miR-185 might be helpful for predicting adverse reactions and effectiveness of SOX regimen against GC.…”

Section: Accumulating Literatures Showed That Alternation Of Non-codingmentioning

confidence: 99%

Gastric juice piR‐1245: A promising prognostic biomarker for gastric cancer

Zhou

Liu

Meng

et al. 2019

Background: Emerging reports demonstrated that PIWI-interacting RNAs (piR-NAs) played an indispensable role in tumorigenesis. However, it still remains elusive whether piR-1245 in gastric juice specific in stomach could be employed as a biomarker for gastric cancer (GC). The present work is aiming at exploring the possibility of piR-1245 in gastric juice as a potential marker to judge for diagnosis and prognosis of gastric cancer. Methods:Gastric juice was collected from 66 GC patients and 66 healthy individuals.Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was employed to measure the levels of piR-1245 expression. Then, the pattern of piR-1245 expression in gastric juice was determined between GC patients and healthy individuals. A receiver operating characteristic (ROC) curve was constructed for distinguishing GC from healthy individuals.Results: Gastric juice piR-1245 levels in GC were higher than those of controls (P < .0001). The value of area under ROC (AUC) was 0.885 (sensitivity, 90.9%; specificity, 74.2%; 95% confidence interval, 0.8286 to 0.9414). High gastric juice piR-1245 expression was signally correlated with tumor size (P = .013) and TNM stage (P = .001). GC patients with high piR-1245 expression in gastric juice exerted a poorer overall survival (OS) (P = .0152) and progression-free survival (PFS) (P = .013). COX regression analysis verified that gastric juice piR-1245 expression was an independent prognostic risk variable for OS (P < .05). Conclusions:The current study suggested that piR-1245 in gastric juice had the potential to be a useful biomarker for GC detection and prognosis prediction. K E Y W O R D Sbiomarker, gastric cancer, gastric juice, piR-1245, PIWI-interacting RNA

“…Gastric cancer (GC) is the fourth global malignant tumors and the fourth leading cause of cancer‐related death worldwide, especially in Eastern Asian countries . Though the overall survival of GC patients to some extent has improved due to the application of early detection technology and radical surgery, most early‐stage gastric cancer (EGC) patients have no characteristic manifestations, and many patients are still diagnosed at the advanced stages and miss the best chance to therapy . Nowadays, upper gastrointestinal (GI) endoscopy followed by pathologic examination remains the golden standard for the diagnosis of GC .…”

Section: Introductionmentioning

confidence: 99%

“…1 Though the overall survival of GC patients to some extent has improved due to the application of early detection technology and radical surgery, most early-stage gastric cancer (EGC) patients have no characteristic manifestations, and many patients are still diagnosed at the advanced stages and miss the best chance to therapy. 2 Nowadays, upper gastrointestinal (GI) endoscopy followed by pathologic examination remains the golden standard for the diagnosis of GC. 3 However, upper GI endoscopy is not a routine medical examination in most countries, and making a gastroscopy inspection is an invasive technique that will sometimes make patients uncomfortable.…”

Section: Introductionmentioning

confidence: 99%

Reduced expression of circRNA hsa_circ_0067582 in human gastric cancer and its potential diagnostic values

Ding

Yang

et al. 2019

Self Cite

Background Gastric cancer (GC) is one of the global mortality diseases and has a poor prognosis due to the lack of ideal tumor biomarkers. Circular RNAs (circRNAs) are an abundant kind of endogenous RNAs that recently are found play a crucial role in the cancer occurrence and development. Nevertheless, little is known with regard to the diagnostic values of these circRNAs for GC. In this article of research, we investigated the role of hsa_circ_0067582 in clinical diagnosis of GC. Materials and Methods We used divergent primers, and the expression levels of hsa_circ_0067582 in 93 fresh GC tissues and paired adjacent normal tissues from surgical patients were detected using quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Then, a receiver operating characteristic (ROC) curve was established to assess the diagnostic significance of hsa_circ_0067582. The relationship between expression of hsa_circ_0067582 and clinicopathological factors of patients was made further explored. Results Hsa_circ_0067582 levels were significantly decreased in GC tissues contrasted with adjacent normal tissues (n = 93, P < .001). After that, we discovered that it was evidently downregulated in 81.7% (76/93) GC tissues. The area under the ROC curve (AUC) of hsa_circ_0067582 was up to 0.6937, the sensitivity was 66.67%, and the specificity was 61.29%. Moreover, the hsa_circ_0067582 levels were obviously associated with tumor diameter (P = .002) and carbohydrate antigen 19‐9 (CA19‐9, P = .01). Meanwhile, after operation, low‐level group of hsa_circ_0067582 of GC patients was associated with better prognosis. Conclusion Our data imply that hsa_circ_000067582 may be a potential biomarker for GC diagnosis and prognosis evaluation.