Global DNA methylation and oxidative stress biomarkers in workers exposed to metal oxide nanoparticles

Liou, Saou-Hsing; Wu, Wei‐Te; Liao, Hui-Yi; Chen, Chao‐Yu; Tsai, Ching-Tsorng; Jung, Wei-Ting; Lee, Hui‐Ling

doi:10.1016/j.jhazmat.2017.02.042

Cited by 93 publications

(62 citation statements)

References 48 publications

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“…As mentioned previously, only one study focused on the quantitative analysis of DNA methylation in a real human population occupationally exposed to NP has been published [14]. However, no such report related to this type of exposure and qualitative DNA methylation approach by methylation chips exists.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to global methylation in white blood cells, oxidative DNA damage, and lipid peroxidation were analyzed in workers who were occupationally exposed to metal oxide nanomaterials (TiO 2 , SiO 2 and indium tin oxide (ITO)). Global DNA hypomethylation was detected in a subgroup of ITO exposed workers, along with increased levels of oxidative damage markers [14].…”

Section: Introductionmentioning

confidence: 97%

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DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles

Rössnerová

Hoňková

Pelclová

et al. 2020

IJMS

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The risk of exposure to nanoparticles (NPs) has rapidly increased during the last decade due to the vast use of nanomaterials (NMs) in many areas of human life. Despite this fact, human biomonitoring studies focused on the effect of NP exposure on DNA alterations are still rare. Furthermore, there are virtually no epigenetic data available. In this study, we investigated global and gene-specific DNA methylation profiles in a group of 20 long-term (mean 14.5 years) exposed, nanocomposite, research workers and in 20 controls. Both groups were sampled twice/day (pre-shift and post-shift) in September 2018. We applied Infinium Methylation Assay, using the Infinium MethylationEPIC BeadChips with more than 850,000 CpG loci, for identification of the DNA methylation pattern in the studied groups. Aerosol exposure monitoring, including two nanosized fractions, was also performed as proof of acute NP exposure. The obtained array data showed significant differences in methylation between the exposed and control groups related to long-term exposure, specifically 341 CpG loci were hypomethylated and 364 hypermethylated. The most significant CpG differences were mainly detected in genes involved in lipid metabolism, the immune system, lung functions, signaling pathways, cancer development and xenobiotic detoxification. In contrast, short-term acute NP exposure was not accompanied by DNA methylation changes. In summary, long-term (years) exposure to NP is associated with DNA epigenetic alterations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles

Rössnerová

Hoňková

Pelclová

et al. 2020

IJMS

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“…The significantly increased levels of tyrosine derivatives in PC patients, ie, ortho‐, chloro‐, and nitro‐tyrosine, demonstrated the presence of oxidative and/or nitrosative stress, which is most likely caused by plasmatic protein damage due to the formation of highly reactive species during tumor growth. Oxidative stress along with the disruption of phospholipid membranes were also reflected by a higher level of 8‐isoprostane in PC patients. In addition, we observed a possible damage to genetic information, which was indicated by a slight increase of 8‐hydroxyguanosine and 8‐hydroxydeoxyguanosine in PC patients.…”

Section: Resultsmentioning

confidence: 99%

“…Oxidative stress along with the disruption of phospholipid membranes were also reflected by a higher level of 8‐isoprostane in PC patients. In addition, we observed a possible damage to genetic information, which was indicated by a slight increase of 8‐hydroxyguanosine and 8‐hydroxydeoxyguanosine in PC patients. Although the differences within the leukotriene C/D/E 4 cascade were negligible, the activation of the patient immune system was expressed by the considerably increased level of leukotriene B 4 , the primary inflammation marker …”

Section: Resultsmentioning

confidence: 99%

Chiroptical spectroscopy and metabolomics for blood‐based sensing of pancreatic cancer

et al. 2018

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To enable the early diagnosis of pancreatic cancer, the search for and definition of reliable biomarkers remain a subject of great interest, with the specificity and sensitivity of the currently used biomarkers being below the required values. We tested a novel diagnostic approach for pancreatic cancer based on the specific molecular signature of blood plasma components. To acquire more detailed structural information, structure-sensitive chiroptical methods (electronic circular dichroism and Raman optical activity) were supplemented by conventional Raman and infrared spectroscopies. The obtained spectra were subsequently processed by linear discriminant analysis yielding high values of specificity and sensitivity. In addition, to monitor not only large biomolecules as potential biomarkers but also those of low molecular weight, we conducted an analysis of blood plasma samples by using metabolomics. The achieved results suggest a panel of promising biomarkers for a reliable detection of pancreatic cancer.

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“…Due to the size-related properties of aerosols (especially respirable-sized particles), the deposited respirable doses in the respirable system have shown potential problems, including invasion, retention and mobility in the human body. That is, in various parts of the respiratory system and the gastrointestinal tract [6,32], they can be transported to other organs of the rodent body through the bloodstream and the nervous system [7,33].…”

Section: Discussionmentioning

confidence: 99%

Human Inhalation Exposure to Aerosol and Health Effect: Aerosol Monitoring and Modelling Regional Deposited Doses

Sohn

Kim

2020

IJERPH

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Since poor air quality affects human health in the short and long term, much research has been performed on indoor and outdoor aerosol exposure; however, there is a lack of specific data on the exposure and health risks of inhalable aerosols that contain bioaerosol in different environments of human life. To investigate the potential exposure to inhalable aerosols (in the monitoring of particulate matter (PM) based on R modeling, variations of PM depend on the ventilation system and bioaerosols based on size distribution) in various environments, the special viability and culturability of bioaerosols and their deposition doses in the respiratory system were evaluated. We conducted exposure assessments on inhalable aerosols in various indoor environments (childcare facilities, schools, commercial buildings, elderly and homes). The fractions of PM (PM10, PM4 and PM2.5) were investigated and, for the bioaerosol, the viability, culturability, inhalation daily dose and the deposited dose of the aerosol in the respiratory system were calculated to evaluate the human health effects. For two years, the distribution of the indoor PM concentration was high in all PM fractions in schools and commercial buildings, and low in the elderly and at homes. For airborne bacteria, the highest concentrations were shown in the childcare facility during the four seasons, while airborne fungi showed high concentrations in the buildings during the spring and summer, which showed significant differences from other investigated environments (between the buildings and elderly and homes: p < 0.05). The viability and culturability for the bioaerosol showed no significant difference in all environments, and the correlation between inhalable PM and bioaerosol obtained from the six-stage impactor showed that the coefficient of determination (R2) between coarse particles (PM10–2.5, the size of stage 2–3) and cultivable airborne bacteria ranged from 0.70 (elderly and homes) to 0.84 (school) during the summer season.

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Global DNA methylation and oxidative stress biomarkers in workers exposed to metal oxide nanoparticles

Cited by 93 publications

References 48 publications

DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles

DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles

Chiroptical spectroscopy and metabolomics for blood‐based sensing of pancreatic cancer

Human Inhalation Exposure to Aerosol and Health Effect: Aerosol Monitoring and Modelling Regional Deposited Doses

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