Global comparative analysis of expressed genes in ovules and leaves of Ginkgo biloba L.

Wang, Li; Lu, Zhaogeng; Li, Weixing; Xu, Jing; Luo, Kaige; Lü, Wei; Zhang, Li; Jin, Biao

doi:10.1007/s11295-016-0989-8

Cited by 15 publications

(15 citation statements)

References 61 publications

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“…It had been reported that leaves collected from different sexes had different antioxidant capacity in Ginkgo (Stefanovits‐Bányai et al ., 2006; Koczka et al ., 2015). Genes with differential expression levels between leaves and ovules have been reported to be involved in flavonoid biosynthesis (Wang et al ., 2016). We found that genes that encode CHS, CHI, F3’H, F3H, DFR, LAR, ANS, and ANR, which are associated with flavonoid biosynthesis, were highly expressed in female leaves (Figure 4(f)), whereas the flavonoid biosynthesis pathway was associated with male floral initiation in Jatropha curcas (Hui et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Its sex differences are embodied in many aspects, including its karyotype (Newcomer, 1954), physiological characteristics (Crane et al ., 2013), and applications (Liao et al ., 2009). Transcriptome analysis has revealed that besides the fact that many genes involved in phytohormone signaling and DNA methylation are differentially expressed between the sexes (Du et al ., 2016), some MADS‐box, WUS, and CLV genes are differentially expressed in male and female reproductive tissues (Wang et al ., 2016). However, the core sex‐determining region in Ginkgo is still unclear, which severely limits elucidation of the molecular mechanism of sex determination.…”

Section: Introductionmentioning

confidence: 99%

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The genomic architecture of the sex‐determining region and sex‐related metabolic variation in Ginkgobiloba

Liao

Gou

et al. 2020

The Plant Journal

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The genomic architecture of the sex‐determining region and sex‐related metabolic variation in Ginkgobiloba

Liao

Gou

et al. 2020

The Plant Journal

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“…In addition, in the ovules of female trees and the ovules of G. biloba var. epiphylla, 34 and 82 conserved miRNAs, respectively, were also identified (Wang et al, 2016;Zhang et al, 2015). In the current study, we identified miRNAs and their targets in a leaf colour mutant of G. biloba, and obtained 213 potentially known miRNAs belonging to 54 miRNA families in mutant leaves that were different from those in the mature normal leaves of G. biloba, and the number was significantly higher than the number of ovules, indicating a diverse array of miRNAs A large majority of plant small RNAs are 21 or 24 nt in length.…”

Section: Discussionmentioning

confidence: 99%

Construction and analysis of a library of miRNA in gold-coloured mutant leaves of Ginkgo biloba L.

Yang

et al. 2019

Folia Horticulturae

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To gain insights into the regulatory networks of miRNAs related to golden colour formation in Ginkgo biloba leaves, we constructed an sRNA library of golden-green striped mutant leaves. A total of 213 known miRNAs comprising 54 miRNA families were obtained, and 214 novel miRNAs were identified in the mutant leaves. We further constructed a normal green leaf sRNA library as a control and compared the expression of miRNAs between mutant and normal leaves. We found 42 known and 54 novel differential expression candidate miRNAs; 39 were up-regulated and 57 down-regulated in mutants compared to normal leaves. Our transcriptome analysis and annotation of the predicted targets indicated that the potential roles of miRNAs in G. biloba leaves included involvement in the ‘Glutathione metabolism’, ‘Plant circadian rhythm’, and ‘Phenylalanine metabolism’ categories. miRNAs and their targets were further validated by qRT-PCR. The expression of miR159a and miR159c, in particular, was significantly higher in mutant leaves than in normal leaves, while their potential target gene CLT3, which is associated with chloroplast development, displayed the opposite expression pattern. In addition, the expression of miR396g-3p and miR396h was also significantly higher in mutant leaves than in normal leaves, while the target genes ABP1 (auxin-related gene) and PPR32 (chloroplast RNA editing protein), respectively, showed the opposite expression pattern. Combined with the transcriptome analysis, these data suggest that miR159, miR396, and their targets may participate in chloroplast development and hormone metabolism to regulate colour formation in G. biloba leaves.

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“…From morphological observations, about 20 petal specimens were cut separately from fertile and sterile flowers at S2, using a razor blade, and ~3 mm 3 of each sample was prefixed in 2.5% (v/v) glutaraldehyde (in 0.1 mol/L phosphate buffer, pH 7.2) at 4°C overnight. After postfixing in 1% (w/v) osmium tetroxide for 6 h at room temperature, the samples were washed three times in 0.2 M phosphate buffer (pH 7.2), dehydrated through an ethanol series, treated twice for 30 min with propylene oxide, and then infiltrated with 1:1 propylene oxide/resin in embedding capsules overnight, before finally embedding in Spurr's resin (Wang et al, 2016 ). For ultrastructural observations, 70 nm-thick sections were cut with a Leica EM UC6 ultramicrotome (Leica Microsystems GmbH, Wetzlar, Germany), and stained with 1% (w/v) uranyl acetate and 1% (w/v) lead citrate.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic Analysis Reveals Mechanisms of Sterile and Fertile Flower Differentiation and Development in Viburnum macrocephalum f. keteleeri

et al. 2017

Front. Plant Sci.

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Sterile and fertile flowers are an important evolutionary developmental (evo-devo) phenotype in angiosperm flowers, playing important roles in pollinator attraction and sexual reproductive success. However, the gene regulatory mechanisms underlying fertile and sterile flower differentiation and development remain largely unknown. Viburnum macrocephalum f. keteleeri, which possesses fertile and sterile flowers in a single inflorescence, is a useful candidate species for investigating the regulatory networks in differentiation and development. We developed a de novo-assembled flower reference transcriptome. Using RNA sequencing (RNA-seq), we compared the expression patterns of fertile and sterile flowers isolated from the same inflorescence over its rapid developmental stages. The flower reference transcriptome consisted of 105,683 non-redundant transcripts, of which 5,675 transcripts showed significant differential expression between fertile and sterile flowers. Combined with morphological and cytological changes between fertile and sterile flowers, we identified expression changes of many genes potentially involved in reproductive processes, phytohormone signaling, and cell proliferation and expansion using RNA-seq and qRT-PCR. In particular, many transcription factors (TFs), including MADS-box family members and ABCDE-class genes, were identified, and expression changes in TFs involved in multiple functions were analyzed and highlighted to determine their roles in regulating fertile and sterile flower differentiation and development. Our large-scale transcriptional analysis of fertile and sterile flowers revealed the dynamics of transcriptional networks and potentially key components in regulating differentiation and development of fertile and sterile flowers in Viburnum macrocephalum f. keteleeri. Our data provide a useful resource for Viburnum transcriptional research and offer insights into gene regulation of differentiation of diverse evo-devo processes in flowers.

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Global comparative analysis of expressed genes in ovules and leaves of Ginkgo biloba L.

Cited by 15 publications

References 61 publications

The genomic architecture of the sex‐determining region and sex‐related metabolic variation in Ginkgobiloba

The genomic architecture of the sex‐determining region and sex‐related metabolic variation in Ginkgobiloba

Construction and analysis of a library of miRNA in gold-coloured mutant leaves of Ginkgo biloba L.

Transcriptomic Analysis Reveals Mechanisms of Sterile and Fertile Flower Differentiation and Development in Viburnum macrocephalum f. keteleeri

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