Transcriptomic Analysis Reveals Mechanisms of Sterile and Fertile Flower Differentiation and Development in Viburnum macrocephalum f. keteleeri

Lu, Zhaogeng; Xu, Jing; Li, Weixing; Zhang, Li; Cui, Jiawen; He, Qingping; Wang, Li; Jin, Biao

doi:10.3389/fpls.2017.00261

Cited by 13 publications

(10 citation statements)

References 71 publications

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“…Cytological observation showed that C. oleifera pollen tube growth inhibition ( Figure 1) after self-pollination is PCD-related [33]. Twenty putative PCD-related genes (10 positive and 10 negative) from literatures [46][47][48][49][50][51] were identified. In Table S5, we can find that 73 PCD-related transcripts were expressed in mature pollen at lower levels than those in self-, cross-and non-pollinated pistils.…”

Section: Identification Of Pcd Candidate Transcriptsin Self-and Crossmentioning

confidence: 99%

“…PCD could be controlled by both positive and negative regulators. In this study, 20 putative key PCD-related genes were detected based on their expression patterns, which included 10 positive genes (ADHIII, AMC4, CAT2, CRY1, RING1, RSP, POB1, SRC2, UBA2C and XCP1) and 10 negative genes (ACA4, BON3, CBSX5, CNX1, CRLK2, IMPA4, P2C63, RBOHA and VPS45) [46][47][48][49][50][51] (Table S5). Most of the positive genes exhibited relatively high levels of expression in SP by Duncan's test, such as CAT2, CRY1, RING1, RSP and UBA2C ( Figure 5A).…”

Section: Pcd-regulated Genes Regulate the Inhibition Of Pollen Tube Imentioning

confidence: 99%

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In-Depth Understanding of Camellia oleifera Self-Incompatibility by Comparative Transcriptome, Proteome and Metabolome

Zhou

et al. 2020

IJMS

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Oil-tea tree (Camellia oleifera) is the most important edible oil tree species in China with late-acting self-incompatibility (LSI) properties. The mechanism of LSI is uncertain, which seriously hinders the research on its genetic characteristics, construction of genetic map, selection of cross breeding parents and cultivar arrangement. To gain insights into the LSI mechanism, we performed cytological, transcriptomic, proteomic and metabolomic studies on self- and cross-pollinated pistils. The studies identified 166,591 transcripts, 6851 proteins and 6455 metabolites. Transcriptomic analysis revealed 1197 differentially expressed transcripts between self- and cross-pollinated pistils and 47 programmed cell death (PCD)-control transcripts. Trend analysis by Pearson correlation categorized nine trend graphs linked to 226 differentially expressed proteins and 38 differentially expressed metabolites. Functional enrichment analysis revealed that the LSI was closely associated with PCD-related genes, mitogen-activated protein kinase (MAPK) signaling pathway, plant hormone signal transduction, ATP-binding cassette (ABC) transporters and ubiquitin-mediated proteolysis. These particular trends in transcripts, proteins and metabolites suggested the involvement of PCD in LSI. The results provide a solid genetic foundation for elucidating the regulatory network of PCD-mediated self-incompatibility in C. oleifera.

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Section: Identification Of Pcd Candidate Transcriptsin Self-and Crossmentioning

confidence: 99%

Section: Pcd-regulated Genes Regulate the Inhibition Of Pollen Tube Imentioning

confidence: 99%

In-Depth Understanding of Camellia oleifera Self-Incompatibility by Comparative Transcriptome, Proteome and Metabolome

Zhou

et al. 2020

IJMS

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“…Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) in accordance with the manufacturer's instructions, and mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. cDNA synthesis was subsequently performed as described previously [21]. RNA sequencing was performed on the Illumina Hiseq 4000 platform.…”

Section: Rna Isolation and Illumina Sequencingmentioning

confidence: 99%

Transcriptomic and Metabolomic Analysis of the Heat-Stress Response of Populus tomentosa Carr.

Ren

et al. 2019

Forests

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Plants have evolved mechanisms of stress tolerance responses to heat stress. However, little is known about metabolic responses to heat stress in trees. In this study, we exposed Populus tomentosa Carr. to control (25 °C) and heat stress (45 °C) treatments and analyzed the metabolic and transcriptomic effects. Heat stress increased the cellular concentration of H2O2 and the activities of antioxidant enzymes. The levels of proline, raffinose, and melibiose were increased by heat stress, whereas those of pyruvate, fumarate, and myo-inositol were decreased. The expression levels of most genes (PSB27, PSB28, LHCA5, PETB, and PETC) related to the light-harvesting complexes and photosynthetic electron transport system were downregulated by heat stress. Association analysis between key genes and altered metabolites indicated that glycolysis was enhanced, whereas the tricarboxylic acid (TCA) cycle was suppressed. The inositol phosphate; galactose; valine, leucine, and isoleucine; and arginine and proline metabolic pathways were significantly affected by heat stress. In addition, several transcription factors, including HSFA2, HSFA3, HSFA9, HSF4, MYB27, MYB4R1, and bZIP60 were upregulated, whereas WRKY13 and WRKY50 were downregulated by heat stress. Interestingly, under heat stress, the expression of DREB1, DREB2, DREB2E, and DREB5 was dramatically upregulated at 12 h. Our results suggest that proline, raffinose, melibiose, and several genes (e.g., PSB27, LHCA5, and PETB) and transcription factors (e.g., HSFAs and DREBs) are involved in the response to heat stress in P. tomentosa.

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“…Our transcriptomic analysis showed that many transcription factors exhibit dynamic gene expression changes in V . macrocephalum f. keteleeri flowers [ 23 ], and some of these genes are miRNA targets. A total of 190 targets of known miRNAs were predicted and annotated with gene descriptions.…”

Section: Discussionmentioning

confidence: 99%

“…After quality control and removing tags from these sources, mapped sRNA tags were used to look for known and novel miRNAs. The sRNA annotation process was performed as follows: 1) The sRNA tags were first mapped to the reference sequence (our transcriptome data sets NCBI SRA [SRP076665] and GEO [GSE83429]) using Bowtie [ 22 , 23 ], without allowing any mismatches, to analyze their expression and distribution relative to the reference sequence. 2) Next, the mapped sRNA tags were used to search for known miRNAs using a modification of the miRDeep2 program (with miRBase used as the reference).…”

Section: Methodsmentioning

confidence: 99%

miRNAs involved in the development and differentiation of fertile and sterile flowers in Viburnum macrocephalum f. keteleeri

Zhang

et al. 2017

BMC Genomics

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BackgroundSterile and fertile flowers are important evolutionary developmental phenotypes in angiosperm flowers. The development of floral organs, critical in angiosperm reproduction, is regulated by microRNAs (miRNAs). However, the mechanisms underpinning the miRNA regulation of the differentiation and development of sterile and fertile flowers remain unclear.ResultsHere, based on investigations of the morphological differences between fertile and sterile flowers, we used high-throughput sequencing to characterize the miRNAs in the differentiated floral organs of Viburnum macrocephalum f. keteleeri. We identified 49 known miRNAs and 67 novel miRNAs by small RNA (sRNA) sequencing and bioinformatics analysis, and 17 of these known and novel miRNA precursors were validated by polymerase chain reaction (PCR) and Sanger sequencing. Furthermore, by comparing the sequencing results of two sRNA libraries, we found that 30 known and 39 novel miRNA sequences were differentially expressed, and 35 were upregulated and 34 downregulated in sterile compared with fertile flowers. Combined with their predicted targets, the potential roles of miRNAs in V. macrocephalum f. keteleeri flowers include involvement in floral organogenesis, cell proliferation, hormonal pathways, and stress responses. miRNA precursors and targets were further validated by quantitative real-time PCR (qRT-PCR). Specifically, miR156a-5p, miR156g, and miR156j expression levels were significantly higher in fertile flowers than in sterile flowers, while SPL genes displayed the opposite expression pattern. Considering that the targets of miR156 are predicted to be SPL genes, we propose that miR156 may be involved in the regulation of stamen development in V. macrocephalum f. keteleeri. ConclusionsWe identified miRNAs differentially expressed between fertile and sterile flowers in V. macrocephalum f. keteleeri and provided new insights into the important regulatory roles of miRNAs in the differentiation and development of fertile and sterile flowers.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4180-x) contains supplementary material, which is available to authorized users.

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Transcriptomic Analysis Reveals Mechanisms of Sterile and Fertile Flower Differentiation and Development in Viburnum macrocephalum f. keteleeri

Cited by 13 publications

References 71 publications

In-Depth Understanding of Camellia oleifera Self-Incompatibility by Comparative Transcriptome, Proteome and Metabolome

In-Depth Understanding of Camellia oleifera Self-Incompatibility by Comparative Transcriptome, Proteome and Metabolome

Transcriptomic and Metabolomic Analysis of the Heat-Stress Response of Populus tomentosa Carr.

miRNAs involved in the development and differentiation of fertile and sterile flowers in Viburnum macrocephalum f. keteleeri

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