Global circular RNA expression profile of human gastric cancer and its clinical significance

Abstract

Circular RNAs (circRNAs) are a new class of noncoding RNAs. However, the expression profile and clinical significance of circRNAs in human gastric cancer is unclear. The global circRNA expression profile in human gastric cancer was measured by circRNA microarray. Hsa_circ_0014717, one of the most downregulated circRNAs in microarray, was selected as a targeted circRNA to explore its levels in gastric tissues and gastric juice. Freeze-thaw experiment and incubation experiment confirmed the stability of gastric juice circRNAs. A total of 308 circRNAs, including 107 (34.74%) upregulated and 201 (65.26%) downregulated circRNAs, were found significantly aberrantly expressed in gastric cancer tissues. The top ten upregulated in gastric cancer tissues were hsa_circ_0035445, hsa_circ_0003789, hsa_circ_0063809, hsa_circ_0074362, hsa_circ_0006282, hsa_ circ_0011107, hsa_circ_0084606, hsa_circ_0005556, hsa_circ_0050547, and hsa_ circ_0006470, while the top ten downregulated ones were hsa_circ_0007099, hsa_circ_0001897, hsa_circ_0007707, hsa_circ_0008832, hsa_circ_0001546, hsa_ circ_0002089, hsa_circ_0004680, hsa_circ_0000154, hsa_circ_0004458, and hsa_ circ_0008394. The hot-point chromosomes were chr1, chr2, chr3, chr9, and chr17. Hsa_circ_0014717 was significantly downregulated in 77.2% (74/96) gastric cancer tissues. Its levels in gastric cancer tissues were related to tumor stage (P = 0.037), distal metastasis (P = 0.048), tissue carcinoembryonic antigen (P = 0.001), and carbohydrate antigen 19-9 expression (P = 0.021). More importantly, hsa_circ_0014717 can stably exist in human gastric juice; and its nature meets the requirements of clinical detection. Our study uncovered the circRNA expression profile in human gastric cancer. Moreover, some circRNAs can stably exist in human body fluid, and has the potential to be used as novel biomarkers for the screening of high-risk gastric cancer patients. Cancer Medicine Open Access 1174

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

“…It was demonstrated that circRNAs are involved in the development of several types of diseases, such as atherosclerosis, myocardial Infarction and neuron injury [10][11][12]. Indeed, there has been a special outpouring of concern for immense amounts of research shown that circRNAs are associated with many human cancers, such as hepatocellular carcinoma [13], esophageal squamous cell carcinoma [14], human gastric cancer [15], colorectal cancer [16]. Our previous study showed that circRNA alterations are involved in resistance to avian leucosis virus subgroup-J-induced tumor formation in chickens [17], however, no reports showed that circRNAs play a key role in chicken cancer which was induced by avian leucosis virus subgroup-J (ALV-J).…”

Global microarray profiling identifiedhsa_circ_0064428as a potential immune-associated prognosis biomarker for hepatocellular carcinoma

“…CircRNAs are widely detected in blood, saliva and body fluid, and their abundance is higher compared with linear RNAs 53 54. Besides, it was reported that circRNA was extremely stable and frequent freeze–thaw does not affect its biological property 55. Recently, studies have indicated that the superiority of circRNAs as prognosis biomarker could reflect the stage characteristics of tumourigenesis 39 48.…”

Landscape of Circular RNAs in the Clinical Application of Digestive System Neoplasm

“…Compared with paired adjacent non-tumorous tissues, hsa_circ_0003159 expression was significantly down-regulated in gastric cancer tissues and significantly negatively associated with gender, distal metastasis, and tumor-node-metastasis stage [34]. Similarly, hsa_circ_0014717 was significantly down regulated as well in gastric cancer tissues and related to tumor stage, distal metastasis, tissue carcinoembryonic antigen and carbohydrate antigen 19-9 expression [35]. However, some circRNAs are upregulated in GC tissues and play a vital role in promoting the development of gastric cancer.…”