Global Chemical Composition and Antioxidant and Anti-Tuberculosis Activities of Various Extracts of Globularia alypum L. (Globulariaceae) Leaves

Abstract

In this work, an evaluation of the biological activities of Globularia alypum L. extracts and their global chemical composition was realized. Extracts from G. alypum were obtained by two extraction methods. The composition of polyphenols (8.5–139.95 g gallic acid equivalent/Kg of dry mass), tannins (1.39–18.65 g catechin equivalent/Kg of dry mass), anthocyanins (8.17–70.69 mg cyanidin equivalent/Kg of dry mass) and flavonoids (0.31–19.28 g quercetin equivalent/Kg of dry mass) was evaluated. The samples were subjected to a screening for their antioxidant activities using the DPPH● and ABTS●+ assays. For the first time, the anti-tuberculosis activity (H37Rv) for G. alypum was tested against Mycobacterium tuberculosis. The strongest antioxidant activity was obtained for the methanol extract (IC50 = 15.58 ± 0.168 mg/L) and the best anti-tuberculosis activity was obtained for the petroleum ether extract (IC50 = 77 mg/L). We have found a positive correlation between the total phenolics content and the antioxidant activity R2 = 0.88 (DPPH●) and R2 = 0.97 (ABTS●+). We have found also a positive correlation between the flavonoid content and the antioxidant activity R2 = 0.91 (DPPH●) and R2 = 0.91 (ABTS●+).

Biological screening of Ajuga iva extracts obtained by supercritical carbon dioxide and pressurized liquid extraction

“…Thus, the variation in the yields of various extracts can be attributed to the polarities of the different compounds. Such differences have been reported in the literature (Khlifi et al, 2011). These findings were in agreement with (El Hilaly et al, 2004) in AI water extract (25% (w/w)), The close finding is reported for other lamiaceae such as T. vulgaris, Salvia officinalis and Origanum majorana (Roby et al, 2013), Thymus numidicus (Ben El Hadj Ali et al, 2014).…”

Plants: A Source for New Antimycobacterial Drugs

“…Compounds such as 34, 61, and 103 act synergistically in combination with commercially available antimycobacterial agents and are paving the way as a new option for multidrug treatment. Compounds 22,23,40,47, and 48 were effective against intracellular dormant bacilli. This review also highlighted some new sites and compounds (40,41,42,55,56,57,61,82, and 103) for drug action, which determine survival of the bacilli.…”

“…Globularia alypum L., Globulariaceae, used in North African folk medicine, is rich in phenols. A study by Khlifi et al in 2011 shows that the methanolic and petroleum ether (PE) extracts of G. alypum leaves contains polyphenols, tannins, anthocyanins, and flavonoids [0. .8 g quercitin (36) equivalent/kg of dry mass] and PE is particularly active against M. tuberculosis (IC 50 77 µg/mL) [23].…”

“…A study by Khlifi et al in 2011 shows that the methanolic and petroleum ether (PE) extracts of G. alypum leaves contains polyphenols, tannins, anthocyanins, and flavonoids [0. .8 g quercitin (36) equivalent/kg of dry mass] and PE is particularly active against M. tuberculosis (IC 50 77 µg/mL) [23]. Lantana camara L., Verbenaceae, contains two new flavonoids, linaroside (37) and lantanoside (38), which cause 30-37 % inhibition of mycobacterial growth at 6.25 µg/mL, which is the MIC of EMB, whereas its common acetylated derivative (39) causes 98% inhibition [24].…”

Antioxidant and antimicrobial activity of a mangrove plant Avicennia marina (Forsk.)

“…The difference in the yield of various solvent extracts can be attributed to the polarity of different compounds in different organs or different plants. Difference in yield in different solvent extracts of different organs of same plants was also reported by Khlifi et al [15] and Moteriya et al [16].…”