Global analysis of lysine succinylation in patchouli plant leaves

Wang, Xiaobing; Chen, Xiuzhen; Li, Junren; Zhou, Xiuping; Liu, Yanting; Zhong, Liting; Tang, Yun; Hai, Zheng; Liu, Jiyun; Zhan, Renya; Chen, Likai

doi:10.1038/s41438-019-0216-5

Cited by 21 publications

(28 citation statements)

References 44 publications

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“…The functional classification and subcellular location distribution of succinylated proteins were compared in three plants (paper mulberry, patchouli [ 45 ] and tea [ 30 ]). The cellular components of the three species are characterized by considerable enrichment in categories related to the cell, membrane, macromolecular complex and organelle membranes.…”

Section: Resultsmentioning

confidence: 99%

“…Chloroplasts are the specific energy conversion organelles of plant cells [ 47 , 48 ] and convert light energy into chemical energy to support plant life. Previous studies of lysine succinylation in wheat [ 49 ], patchouli plant leaves [ 45 ], Brachypodium distachyon L. [ 29 ] and tomato [ 31 ] identified various succinylation sites and proteins related to photosynthesis and demonstrated potential functional regulation of photosynthesis-related proteins by succinylation. Thus, protein succinylation may be a conserved form of regulation of photosynthesis in various plant species.…”

Section: Resultsmentioning

confidence: 99%

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First comprehensive analysis of lysine succinylation in paper mulberry (Broussonetia papyrifera)

Dong

Chen

2021

BMC Genomics

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Background Lysine succinylation is a naturally occurring post-translational modification (PTM) that is ubiquitous in organisms. Lysine succinylation plays important roles in regulating protein structure and function as well as cellular metabolism. Global lysine succinylation at the proteomic level has been identified in a variety of species; however, limited information on lysine succinylation in plant species, especially paper mulberry, is available. Paper mulberry is not only an important plant in traditional Chinese medicine, but it is also a tree species with significant economic value. Paper mulberry is found in the temperate and tropical zones of China. The present study analyzed the effects of lysine succinylation on the growth, development, and physiology of paper mulberry. Results A total of 2097 lysine succinylation sites were identified in 935 proteins associated with the citric acid cycle (TCA cycle), glyoxylic acid and dicarboxylic acid metabolism, ribosomes and oxidative phosphorylation; these pathways play a role in carbon fixation in photosynthetic organisms and may be regulated by lysine succinylation. The modified proteins were distributed in multiple subcellular compartments and were involved in a wide variety of biological processes, such as photosynthesis and the Calvin-Benson cycle. Conclusion Lysine-succinylated proteins may play key regulatory roles in metabolism, primarily in photosynthesis and oxidative phosphorylation, as well as in many other cellular processes. In addition to the large number of succinylated proteins associated with photosynthesis and oxidative phosphorylation, some proteins associated with the TCA cycle are succinylated. Our study can serve as a reference for further proteomics studies of the downstream effects of succinylation on the physiology and biochemistry of paper mulberry.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

First comprehensive analysis of lysine succinylation in paper mulberry (Broussonetia papyrifera)

Dong

Chen

2021

BMC Genomics

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“…Succinylation alters the electrophoretic mobility and isoionic pH of ovalbumin [ 140 ], inactivates the canonical carnitine palmitoyltransferase (CPTase) activity, decreases the enolase activity [ 141 ], and promotes cell invasion and migration [ 139 ]. Succinylation in plants has been reported in rice [ 34 ], tomato [ 142 ], B. distachyon L. [ 135 ], patchouli [ 143 ], and tea [ 144 ]. However, the effect of lysine succinylation in plants is relatively limited.…”

Section: Starch Biosynthesis-related Proteins Targeted By Ptmsmentioning

confidence: 99%

Proteomics and Post-Translational Modifications of Starch Biosynthesis-Related Proteins in Developing Seeds of Rice

Tappiban

Bao

2021

IJMS

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Rice (Oryza sativa L.) is a foremost staple food for approximately half the world’s population. The components of rice starch, amylose, and amylopectin are synthesized by a series of enzymes, which are responsible for rice starch properties and functionality, and then affect rice cooking and eating quality. Recently, proteomics technology has been applied to the establishment of the differentially expressed starch biosynthesis-related proteins and the identification of posttranslational modifications (PTMs) target starch biosynthesis proteins as well. It is necessary to summarize the recent studies in proteomics and PTMs in rice endosperm to deepen our understanding of starch biosynthesis protein expression and regulation, which will provide useful information to rice breeding programs and industrial starch applications. The review provides a comprehensive summary of proteins and PTMs involved in starch biosynthesis based on proteomic studies of rice developing seeds. Starch biosynthesis proteins in rice seeds were differentially expressed in the developing seeds at different developmental stages. All the proteins involving in starch biosynthesis were identified using proteomics methods. Most starch biosynthesis-related proteins are basically increased at 6–20 days after flowering (DAF) and decreased upon the high-temperature conditions. A total of 10, 14, 2, 17, and 7 starch biosynthesis related proteins were identified to be targeted by phosphorylation, lysine acetylation, succinylation, lysine 2-hydroxyisobutyrylation, and malonylation, respectively. The phosphoglucomutase is commonly targeted by five PTMs types. Research on the function of phosphorylation in multiple enzyme complex formation in endosperm starch biosynthesis is underway, while the functions of other PTMs in starch biosynthesis are necessary to be conducted in the near future.

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“…In plants, chloroplast photosynthesis is an important biochemical reaction that converts light energy into chemical energy to maintain plant life [49]. Research on plant defense and photosynthesis has indicated that the rate of photosynthesis is reduced after pathogen invasion, such as in barley infected with powdery mildew, potato infected with Phytophthora infestans, and soybean infected with Phytophthora sojae [50][51][52].…”

Section: Defensive Photosynthetic Activities Of Wheat Stem Bases Undementioning

confidence: 99%

TMT-based quantitative proteomic analysis reveals defense mechanism of wheat against the crown rot pathogen Fusarium pseudograminearum

Qiao

Yang

et al. 2021

BMC Plant Biol

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Background Fusarium crown rot is major disease in wheat. However, the wheat defense mechanisms against this disease remain poorly understood. Results Using tandem mass tag (TMT) quantitative proteomics, we evaluated a disease-susceptible (UC1110) and a disease-tolerant (PI610750) wheat cultivar inoculated with Fusarium pseudograminearum WZ-8A. The morphological and physiological results showed that the average root diameter and malondialdehyde content in the roots of PI610750 decreased 3 days post-inoculation (dpi), while the average number of root tips increased. Root vigor was significantly increased in both cultivars, indicating that the morphological, physiological, and biochemical responses of the roots to disease differed between the two cultivars. TMT analysis showed that 366 differentially expressed proteins (DEPs) were identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment in the two comparison groups, UC1110_3dpi/UC1110_0dpi (163) and PI610750_3dpi/PI610750_0dpi (203). It may be concluded that phenylpropanoid biosynthesis (8), secondary metabolite biosynthesis (12), linolenic acid metabolites (5), glutathione metabolism (8), plant hormone signal transduction (3), MAPK signaling pathway-plant (4), and photosynthesis (12) contributed to the defense mechanisms in wheat. Protein-protein interaction network analysis showed that the DEPs interacted in both sugar metabolism and photosynthesis pathways. Sixteen genes were validated by real-time quantitative polymerase chain reaction and were found to be consistent with the proteomics data. Conclusion The results provided insight into the molecular mechanisms of the interaction between wheat and F. pseudograminearum.

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Global analysis of lysine succinylation in patchouli plant leaves

Cited by 21 publications

References 44 publications

First comprehensive analysis of lysine succinylation in paper mulberry (Broussonetia papyrifera)

First comprehensive analysis of lysine succinylation in paper mulberry (Broussonetia papyrifera)

Proteomics and Post-Translational Modifications of Starch Biosynthesis-Related Proteins in Developing Seeds of Rice

TMT-based quantitative proteomic analysis reveals defense mechanism of wheat against the crown rot pathogen Fusarium pseudograminearum

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