Gliocyte and synapse analyses in cerebral ganglia of the Chinese mitten crab, Eriocheir sinensis: ultrastructural study

Zhang, H.; Yu, Peng; Zhong, Shengwei; Ge, Tingting; Peng, Shasha; Zhou, Ziyan; Guo, Xiaoquan

doi:10.4081/ejh.2016.2655

Cited by 8 publications

(4 citation statements)

References 32 publications

(21 reference statements)

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“…In our traditional cognition, only nerve cells are involved in neural communication. Increasing shreds of evidence show that gliocytes also play an essential role in neural communication, although the effect of gliocytes on the whole process remains to be understood ( Liu and Wang, 2016 ; Zhang et al, 2016 ). In this case, cell manipulation techniques provide a promising way to study cell communication at a single cell level ( Yun et al, 2013 ).…”

Section: Advanced Techniques For Ex Vivo Non Studiesmentioning

confidence: 99%

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Zhang

Rong

Bian

2022

Front. Bioeng. Biotechnol.

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Increasing population is suffering from neurological disorders nowadays, with no effective therapy available to treat them. Explicit knowledge of network of neurons (NoN) in the human brain is key to understanding the pathology of neurological diseases. Research in NoN developed slower than expected due to the complexity of the human brain and the ethical considerations for in vivo studies. However, advances in nanomaterials and micro-/nano-microfabrication have opened up the chances for a deeper understanding of NoN ex vivo, one step closer to in vivo studies. This review therefore summarizes the latest advances in lab-on-chip microsystems for ex vivo NoN studies by focusing on the advanced materials, techniques, and models for ex vivo NoN studies. The essential methods for constructing lab-on-chip models are microfluidics and microelectrode arrays. Through combination with functional biomaterials and biocompatible materials, the microfluidics and microelectrode arrays enable the development of various models for ex vivo NoN studies. This review also includes the state-of-the-art brain slide and organoid-on-chip models. The end of this review discusses the previous issues and future perspectives for NoN studies.

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Section: Advanced Techniques For Ex Vivo Non Studiesmentioning

confidence: 99%

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Zhang

Rong

Bian

2022

Front. Bioeng. Biotechnol.

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“…The TEM protocol was conducted in accordance with our previous methods. [12][13][14] The myocardia were sampled and fixed in 2.5% glutaraldehyde/PBS (pH 7.4, 0.1 M) at 4°C for 48 h, and then they were postfixed in 1% OsO 4 (Polysciences Inc., Warrington, PA, USA) for 1 h, dehydrated in a concentration series of ethanol, infiltrated with propylene oxide/Araldite mixture and embedded in Araldite. The samples were sectioned, and they were stained with 1% uranyl acetate and Reynold's lead citrate for 20 min.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Ultrastructure of telocytes, a new type of interstitial cells in the myocardium of the Chinese giant salamander (Andrias davidianus)

Zhang

2019

Eur J Histochem

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Telocytes (TCs) are new interstitial cells, and they are involved in tissue regeneration, particularly in heart. Therefore, TCs are suggested to be a promising cell in regenerative medicine. However, the information of location structural characteristics and functions of TCs is still limited. In this study, cardiac TCs of the Chinese giant salamanders (Andrias davidianus) were identified by transmission electron microscopy. TCs were located in the interstitium between cardiomyocytes (CM). TCs possessed distinctive ultrastructural characteristics, including one to two very long and thin moniliform telopodes (Tps), emerging points from the cell body, caveolae, dichotomous branchings, labyrinthic systems, neighbouring exosomes and homo-cellular contacts between Tps. TCs/Tps were frequently observed in close proximity to cardiomyocytes. Moreover, Tps established hetero-cellular contacts with cardiomyocytes. Our results confirm the presence of TCs in the myocardium of the A. davidianus. This will help us to better understand roles of TCs in amphibian hearts.

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“…[37][38][39][40][41][42] The biology of stem cells was extensively investigated through the labelling by specific molecular markers, during development and in the adult. [43][44][45][46] The molecular organization of cells and tissues was carefully analyzed [47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63] especially in poorly described species of mammals [55][56][57] and non-mammalian vertebrates [58][59][60][61] or in invertebrates, 62,63 while immunohistochemistry was crucial to elucidate the topographic distribution of cell lineages in different organs, [64][65][66] especially in the nervous system, [67][68][69][70][71] and during embryogenesis and the pre-or postnatal development, [72][73]…”

Section: Applications Of Histochemistry In the Recent Scientific Literaturementioning

confidence: 99%

Histochemistry as a versatile research toolkit in biological research, not only an applied discipline in pathology

Pellicciari

2018

Eur J Histochem

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The impressive progress of histochemistry over the last 50 years has led to setting up specific and sensitive techniques to describe dynamic events, through the detection of specific molecules in the very place where they exist in live cells. The scientific field where histochemistry has most largely been applied is histopathology, with the aim to identify disease-specific molecular markers or to elucidate the etiopathological mechanisms. Numerous authors did however apply histochemistry to a variety of other research fields; their interests range from the microanatomy of animal and plant organisms to the cellular mechanisms of life. This is especially apparent browsing the contents of the histochemical journals where the articles on subjects other than pathology are the majority; these journals still keep a pivotal role in the field of cell and tissue biology, while being a forum for a diverse range of biologists whose scientific interests expand the research horizon of histochemistry to ever novel subjects. Thus, histochemistry can always receive inspiring stimuli toward a continuous methodological refinement.

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Gliocyte and synapse analyses in cerebral ganglia of the Chinese mitten crab, Eriocheir sinensis: ultrastructural study

Cited by 8 publications

References 32 publications

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Ultrastructure of telocytes, a new type of interstitial cells in the myocardium of the Chinese giant salamander (Andrias davidianus)

Histochemistry as a versatile research toolkit in biological research, not only an applied discipline in pathology

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