Glial cells from adult rat olfactory bulb: Immunocytochemical properties of pure cultures of ensheathing cells

Ramón‐Cueto, Almudena; Nieto–Sampedro, Manuel

doi:10.1016/0306-4522(92)90134-n

Cited by 203 publications

(149 citation statements)

References 40 publications

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“…1 A) and Western blotting ( Fig. 1 B) (Ramon-Cueto and Nieto-Sampedro, 1992;Perroteau et al, 1998;Pollock et al, 1999;Gilbert et al, 2001;Moreno-Flores et al, 2003b). Proteins such as S100␤, p75 NTR , 3PGDH, APP, and ErbB2 were expressed similarly in all three OEC populations.…”

Section: Characterization Of Three Distinct Oec Populationsmentioning

confidence: 83%

Genes Associated with Adult Axon Regeneration Promoted by Olfactory Ensheathing Cells: A New Role for Matrix Metalloproteinase 2

et al. 2006

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The molecular mechanisms used by olfactory ensheathing cells (OECs) to promote repair in the damaged adult mammalian CNS remain unknown. Thus, we used microarrays to analyze three OEC populations with different capacities to promote axonal regeneration in cultured rat retinal neurons. Gene expression in "long-term cultured OECs" that do not stimulate adult axonal outgrowth was compared with that of "primary olfactory ensheathing cells" and the immortalized OEC cell line TEG3. In this way, we identified a number of candidate genes that might play a role in promoting adult axonal regeneration. Among these genes, it was striking that both the matrix metalloproteinase 2 (MMP2) and an inhibitor of this protease were represented. The disruption of MMP2 activity in TEG3 cells impaired their capacity to trigger axon regeneration in cultured adult retinal neurons. Furthermore, the MMP2 protein was detected in grafts of OECs that elicited robust axonal regeneration in the injured spinal cord of adult rats in vivo. These data suggest that MMP2 does indeed participate in adult axonal regeneration induced by OECs.

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Section: Characterization Of Three Distinct Oec Populationsmentioning

confidence: 83%

Genes Associated with Adult Axon Regeneration Promoted by Olfactory Ensheathing Cells: A New Role for Matrix Metalloproteinase 2

et al. 2006

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“…Although previous reports have demonstrated that growth factors secreted from OECs such as nerve growth factor (NGF), BDNF, GDNF, and neurturin (NTN) might indirectly facilitate axon regeneration (52), the actual molecules involved in the cell-cell contact mechanism that directly mediated the neurite outgrowth-stimulating effect of hOECs/ONFs are not completely known. Some investigations have found that cell adhesion molecules, such as L1 and N-cadherin, were associated with the neurite outgrowth (53,54). Furthermore, it is known that PrP C plays an important role in the regulation of neurite regeneration (23).…”

Section: Figurementioning

confidence: 99%

Implantation of olfactory ensheathing cells promotes neuroplasticity in murine models of stroke

et al. 2008

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Murine olfactory ensheathing cells (OECs) promote central nervous system axonal regeneration in models of spinal cord injury. We investigated whether OECs could induce a neuroplastic effect to improve the neurological dysfunction caused by hypoxic/ischemic stress. In this study, human OECs/olfactory nerve fibroblasts (hOECs/ONFs) specifically secreted trophic factors including stromal cell-derived factor-1α (SDF-1α). Rats with intracerebral hOEC/ONF implantation showed more improvement on behavioral measures of neurological deficit following stroke than control rats.

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“…This capacity is thought to be due in part to the unique glial cells of the olfactory nerve, known as olfactory ensheathing cells (ECs). These cells share phenotypic characteristics in common with Schwann cells and astrocytes [5,15,41,47] but are unique in that they can cross the transitional zone between the peripheral nervous system and CNS [17,45]. Although it is well documented that ECs express extracel lular matrix molecules such as N-CAM and laminin [22,33] that could contribute to axonal growth, few studies 204 E. Woodhall et al f Molecular Brain Research 88 (2001) [203][204][205][206][207][208][209][210][211][212][213] have shown unequivocal direct evidence that ECs syn thesize and secrete growth factors [13].…”

Section: Introductionmentioning

confidence: 99%

Cultured olfactory ensheathing cells express nerve growth factor, brain-derived neurotrophic factor, glia cell line-derived neurotrophic factor and their receptors

Woodhall

West

Chuah

2001

Molecular Brain Research

306

209

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In the primary olfactory pathway axons of olfactory neurons (ONs) are accompanied by ensheathing cells (ECs) as the fibres course towards the olfactory bulb. Ensheathing cells are thought to play an important role in promoting and guiding olfactory axons to their appropriate target. In recent years, studies have shown that transplants of ECs into lesions in the central nervous system (CNS) are able to stimulate the growth ofaxons and in some cases restore functional connections. In an attempt to identify a possible mechanism underlying EC support for olfactory nerve growth and CNS axonal regeneration, this study investigated the production of growth factors and expression of corresponding receptors by these cells. Three techniques immunohistochemistry, enzyme linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to assess growth factor expression in cultured ECs. Immunohistochemistry showed that ECs expressed nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and glial cell-line derived neurotrophic factor (GDNF). ELISA confirmed the intracellular presence of NGF and BDNF and showed that, compared to BDNF, about seven times as much NGF was secreted by ECs. RT-PCR analysis demonstrated expression of mRNA for NGF, BDNF, GDNF and neurturin (NTN). In addition, ECs also expressed the receptors trkB, GFRa-1 and GFRa-2. The results of the experiments show that ECs express a number of growth factors and that BDNF in particular could act both in a paracrine and autocrine manner.

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Glial cells from adult rat olfactory bulb: Immunocytochemical properties of pure cultures of ensheathing cells

Cited by 203 publications

References 40 publications

Genes Associated with Adult Axon Regeneration Promoted by Olfactory Ensheathing Cells: A New Role for Matrix Metalloproteinase 2

Genes Associated with Adult Axon Regeneration Promoted by Olfactory Ensheathing Cells: A New Role for Matrix Metalloproteinase 2

Implantation of olfactory ensheathing cells promotes neuroplasticity in murine models of stroke

Cultured olfactory ensheathing cells express nerve growth factor, brain-derived neurotrophic factor, glia cell line-derived neurotrophic factor and their receptors

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