Implantation of olfactory ensheathing cells promotes neuroplasticity in murine models of stroke

Shyu, Woei Cherng; Liu, Demeral David; Lin, Shinn Zong; Li, Wen Wen; Su, Ching Yuan; Chang, Ying Chen; Wang, Hsiao Jung; Wang, Hsing Won; Tsai, Chang Hai; Li, Hung

doi:10.1172/jci34363

Cited by 89 publications

(83 citation statements)

References 85 publications

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“…Data obtained using various injury models support the view that OEC transplants can reduce cavitation, increase axonal sprouting and regeneration, and a moderate degree of functional motoric recovery (Li et al, 1997;Ramon-Cueto et al, 2000;Collazos-Castro et al, 2005;Lopez-Vales et al, 2007;Sasaki et al, 2007). Moreover, transplanted OECs promote neuroplasticity in murine models of stroke (Shyu et al, 2008), restore functional deficits in rat model of Parkinson's disease (Agrawal et al, 2004), and OECs have been transplanted to an amyotrophic lateral sclerosis (ALS) mouse model (Morita et al, 2008). In clinic, Lima et al (2006) and Feron et al (2005) used adult autologous olfactory mucosa OECs from patients to repair spinal cord injury.…”

supporting

confidence: 53%

The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration

Liu²,

Wang³

et al. 2010

The Anatomical Record

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Clinical studies have expanded the therapeutic olfactory ensheathing cells (OECs) transplantation to different human Central Nervous System (CNS) diseases. In fact, the OEC transplantation in clinic is a mixture of olfactory bulb cells; they even have not demonstrated that they have such a subpopulation yet. However, as a source of OECs transplantation, the development and identification of human fetal OECs are still need more understanding, because some surgery try to restoration CNS injury with a more purity of OEC cultures generated by a number of different procedures. In this article, twelve human fetal olfactory bulb (OB) samples were obtained from six fetuses in 20 weeks of gestation, it was studied by immunofluorescence on histological sections and cultured cells with multiple antibodies under confocal microscopy. The P75NTR positive OB-OECs (olfactory ensheathing cell from the olfactory bulb) were present in both outer olfactory nerve layers and glomerular layer. The percentage of OB cells in culture, about 22.31 was P75NTR positive, 45.77 was S100b, and 31.92 was GFAP. P75NTR and GFAP were coexpressed with S100b, respectively; however, P75NTR was not coexpressed with GFAP in human fetal OECs. It is suggested that the localization and development of human OECs in OB are different to those in rodent, and the P75NTR immunohistological staining is still necessary to identify and characterize human fetal OECs in culture before transplantation. Anat Rec, 293:359-369, 2010. V V C 2009 Wiley-Liss, Inc.

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supporting

confidence: 53%

The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration

Liu²,

Wang³

et al. 2010

The Anatomical Record

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“…The olfactory mucosa is a permanently self-renewing nervous tissue, even in elderly persons, which harbors a variety of cells supporting both its normal function and its regenerative capacity (18). Olfactory ensheathing cells, involved in axonal outgrowth guidance, have already been described as a valid tool to promote neuroplasticity after brain transplantation (19). Thus, diverting cells of the highly plastic peripheral olfactory system toward a poorly self-renewing area appears as a potential means of treatment of the injured nervous system.…”

Section: Introductionmentioning

confidence: 99%

Engraftment of human nasal olfactory stem cells restores neuroplasticity in mice with hippocampal lesions

Nivet¹,

Vignes²,

Girard³

et al. 2011

J. Clin. Invest.

114

107

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Stem cell-based therapy has been proposed as a potential means of treatment for a variety of brain disorders. Because ethical and technical issues have so far limited the clinical translation of research using embryonic/ fetal cells and neural tissue, respectively, the search for alternative sources of therapeutic stem cells remains ongoing. Here, we report that upon transplantation into mice with chemically induced hippocampal lesions, human olfactory ecto-mesenchymal stem cells (OE-MSCs) -adult stem cells from human nasal olfactory lamina propria -migrated toward the sites of neural damage, where they differentiated into neurons. Additionally, transplanted OE-MSCs stimulated endogenous neurogenesis, restored synaptic transmission, and enhanced long-term potentiation. Mice that received transplanted OE-MSCs exhibited restoration of learning and memory on behavioral tests compared with lesioned, nontransplanted control mice. Similar results were obtained when OE-MSCs were injected into the cerebrospinal fluid. These data show that OE-MSCs can induce neurogenesis and contribute to restoration of hippocampal neuronal networks via trophic actions. They provide evidence that human olfactory tissue is a conceivable source of nervous system replacement cells. This stem cell subtype may be useful for a broad range of stem cell-related studies.

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“…In addition, OECs can secrete many types of nutrition factors, such as nerve growth factor, neurotrophic cytokines, and brain-derived neurotrophic factor; these factors promote the development, differentiation, and survival of neurons. Thus, OECs provide a good internal environment for injured nerve repair, nerve regeneration, and functional recovery (Shyu et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of olfactory ensheathing cell transplantation for treatment of spinal cord injury

Rao

Zhu

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to determine the effectiveness and safety of transplantation of olfactory ensheathing cells for functional repair of the spinal cord. An olfactory bulb was obtained from a 4-to 5-month-old aborted fetus, and it was digested into single olfactory ensheathing cells and then cultured and purified for 1 to 2 weeks. Under general anesthesia, these single-cell suspensions of olfactory ensheathing cells were injected into the corresponding spinal injury site with 0.45-mm-diameter injections. The American Spinal Injury Association (ASIA) Impairment Scale was used to evaluate spinal function. A total of 15 patients (12 men, 3 women; age range, 18-56 years; mean age, 40) were admitted for obsolete spinal injuries. Spinal functions of the 15 patients were observed and followed postoperatively for a period ranging from 2 weeks to 1 month. All the 15 patients exhibited improvements in spinal function, and the improvement tendencies continued. Twelve patients had obvious spinal function improvement, and three had slight improvement according to the ASIA scale, with an 4125 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 4124-4129 (2014) Olfactory ensheathing cell and spinal cord injury obvious difference between preoperation and postoperation measures (P < 0.05). No fevers, infections, functional deteriorations, or deaths were seen. Thus, transplantation of olfactory ensheathing cells promoted spinal and neurofunctional recovery in patients with malignant spinal injuries, and this therapeutic method was safe.

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Implantation of olfactory ensheathing cells promotes neuroplasticity in murine models of stroke

Cited by 89 publications

References 85 publications

The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration

The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration

Engraftment of human nasal olfactory stem cells restores neuroplasticity in mice with hippocampal lesions

Effectiveness of olfactory ensheathing cell transplantation for treatment of spinal cord injury

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