Glial cell derived neurotrophic factor induces spermatogonial stem cell marker genes in chicken mesenchymal stem cells

Boozarpour, Sohrab; Matin, Maryam Moghaddam; Momeni-Moghaddam, Madjid; Dehghani, Hesam; Mahdavi-Shahri, Naser; Sisakhtnezhad, Sajjad; Heirani‐Tabasi, Asieh; Irfan-Maqsood, Muhammad; Bahrami, Ahmad Reza

doi:10.1016/j.tice.2016.03.003

Cited by 5 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mesenchymal stromal cells (MSCs) are adult, multipotent progenitor cells that have been isolated from many species, including chickens (1). In chickens, MSCs have previously been collected from bone marrow, umbilical cord, skin, amniotic tissue, lung and intestine (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). These chicken MSCs exhibited the potential to differentiate into adipocytes, chondrocytes, and osteocytes, and have been reported to express the cell surface markers CD29, CD44, CD73 and CD90, but not CD31 and CD34, consistent with MSC data from other species.…”

Section: Introductionmentioning

confidence: 99%

“…These chicken MSCs exhibited the potential to differentiate into adipocytes, chondrocytes, and osteocytes, and have been reported to express the cell surface markers CD29, CD44, CD73 and CD90, but not CD31 and CD34, consistent with MSC data from other species. Moreover, chicken MSCs have been shown to have the potential to differentiate into beta-like pancreatic islet and spermatogonial cells ( 6 , 13 ). Functionally, chicken bone marrow (BM)-derived MSC were found to exhibit an anti-tumoral effect by inducing apoptosis in tumor cell lines ( 3 ), and chicken lung-derived MSC suppressed T-cell activity ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties

et al. 2022

View full text Add to dashboard Cite

Mesenchymal stromal cells (MSCs) are adult multipotent progenitor cells that have been isolated from various tissue sources of many species, primarily mammals. Generally, these cells proliferate extensively in culture and have been shown to secrete bioactive factors that contribute to healing processes by regulating inflammation, modulating immune responses, inhibiting bacterial growth, and promoting tissue regeneration. The present study reports on the isolation and characterization of MSCs from the peripheral blood (PB) of chickens. Chicken PBMSCs were characterized based on their trilineage differentiation potential and gene and protein expression of MSC-specific cell surface markers. To determine functionality, conditioned medium (CM), which contains all bioactive factors secreted by MSCs, was collected from chicken PBMSCs, and used in in vitro antimicrobial, migration, and angiogenesis assays. Chicken PBMSC CM was found to (i) inhibit the growth of planktonic Staphylococcus aureus (S. aureus), and even more significantly the methicillin-resistant S. aureus (MRSA), (ii) decrease adhesion and promote migration of fibroblasts, and (iii) support endothelial cell tube formation. Collectively, these data indicate that chicken PBMSCs secrete bioactive factors with antimicrobial and regenerative properties, and as such, provide a novel source of cell-based therapies for the poultry industry.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In general, these growth factors enhance the expression of the early spermatogenic and pre-meiosis markers. Boozarpour et al also reported that c-Kit was downregulated in the RA treatment group after 14 days, but this gene was overexpressed in the presence of GDNF (15 ng/ml) (Boozarpour et al, 2016). with Sertoli cells and used RA and testosterone for the differentiation of stem cells into germ cells.…”

Section: Discussion and Con Clus I Onmentioning

confidence: 99%

“…In the past decade, many studies were done to differentiate stem cells, especially MSCs to the germ cells and hopeful results were obtained (Boozarpour et al, 2016;Ghorbanlou et al, 2019;Xie et al, 2015). To increase the efficiency of the differentiation, studies are performed to provide a suitable niche for stem cells.…”

Section: Discussion and Con Clus I Onmentioning

confidence: 99%

Differentiation potential of adipose tissue‐derived mesenchymal stem cells into germ cells with and without growth factors

et al. 2020

View full text Add to dashboard Cite

This study aimed to culture the adipose tissue-derived mesenchymal stem cells (AT-MSCs) with and without leukaemia inhibitory factor (LIF), glial cell line-derived neurotrophic factor (GDNF), epidermal growth factor (EGF) and retinoic acid (RA), and investigate their impact on the differentiation of these cells into germ cells. MSCs were separated from adipose tissue of mice, and the nature of these cells is confirmed by flow cytometry. The cells were cultured in different conditions, including MSCs grown in the presence of the growth factors, MSCs without the growth factors, MSCs cultured with combined growth factors and RA, and MSCs cultured with RA. After 2 weeks, the gene expression of c-Kit, Gcnf, Mvh and Scp3 and the protein expression of c-Kit and Gcnf were assessed by real-time PCR and Western blot, respectively. Scp3 was overexpressed in the groups supplemented with RA (p < .01). The expression of c-Kit and Mvh in the growth factor-supplemented groups was increased (p < .01). Western blot analysis confirmed the real-time PCR results. The use of the growth factors for the long-term culture of stem cells can be beneficial. However, to promote germ cell differentiation, the growth factors might be used by other meiosis inducer factors, such as RA.

show abstract

“…MSCs are relatively easier to isolate from bone marrow due to their adherence to plastic and expansion in culture [7]. Therefore, differential adhesion method is one of the commonest methods to isolate chicken MSCs (cMSCs) from bone marrow, but the time of differential adhesion are usually up to 24 h in previous study [8][9][10]. This is the rst study to shorten the time to 4 h, which greatly improved the e ciency of isolation.MSCs can differentiate into various cell linages in vitro when being treated with special factors such as cytokines, growth factor and inductive agents [11][12][13][14].…”

mentioning

confidence: 99%

Myogenic differentiation potential of chicken mesenchymal stem cells from bone marrow

Zhou

Zhao

Cai

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Mesenchymal stem cells (MSCs) have the potential to multilineage differentiation, which can be used for a good model to provide critical insight of chicken muscle development. Differential adhesion method is one of the commonest methods to isolate MSCs based on the ability of plastic adhesion. 5-azacytidine (5-Aza), dexamethasone (DXMS), hydrocortisone (HC) and horse serum had been proved the potential to induce the myogenic differentiation of MSCs. However, the myogenic differentiation of MSCs is still poorly understood in chicken. In present study, we isolated chicken mesenchymal stem cells (cMSCs) from bone using 4-hour differential adhesion method and analyzed the myogenic effect of cMSCs treated with different method based on 5-Aza, DXMS, HC and horse serum.Results: cMSCs isolated by 4-hour differential adhesion method expressed MSCs special surface markers and presented normal growth characteristic. cMSCs showed great potential of myogenic differentiation by the treatment of 5-Aza and horse serum. RNA-sequence, GO and KGEE enrichment analysis revealed that this effect might be based on demethylation of 5-Aza and ECM-receptor interaction, focal adhesion, PI3K-Akt, p53, TGF-beta signaling pathways. Moreover, DXMS, HC and horse serum also presented potential of myogenic differentiation, but the effect was not as good as 5-Aza and horse serum method.Conclusions: cMSCs showed potential of myogenic differentiation by the treatment of 5-Aza and horse serum or DXMS, HC and horse serum.

show abstract

Glial cell derived neurotrophic factor induces spermatogonial stem cell marker genes in chicken mesenchymal stem cells

Cited by 5 publications

References 37 publications

Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties

Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties

Differentiation potential of adipose tissue‐derived mesenchymal stem cells into germ cells with and without growth factors

Myogenic differentiation potential of chicken mesenchymal stem cells from bone marrow

Contact Info

Product

Resources

About