Glial and endothelial cell response to a fetal transplant of purified neurons

Rostaing-Rigattieri, S.; Flores-Guevara, R; Peschanski, Marc; Cadusseau, Josette

doi:10.1016/s0306-4522(96)00671-9

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…Factors released from the embryonic graft tissue could also play a similar inhibitory role. Indeed, a recent study suggests that intrathalamic grafts of purified motor neurons (devoid of glial cells) suppress host astrocytes and microglia, that have been activated by excitotoxin injection, and make them adopt a quiescent morphology (Rostaing‐Rigattieri et al . 1997).…”

Section: Discussionmentioning

confidence: 99%

“…There is considerable evidence that intracerebral injection of an excitotoxin such as ibotenate (Coffey et al, 1990;Isacson et al, 1987), kainate (Andersson et al, 1991;Dusart et al, 1991;Marty et al, 1991;Finsen et al, 1993;Jørgensen et al, 1993;Rostaing-Rigattieri et al, 1997) or quinolinic acid (Nakao et al, 1996) not only kills neurons in the striatum but also causes an intense inflammation. In a group of control rats of the present study, we found a massive inflammation 9 days following intrastriatal QUIN infusion.…”

Section: Discussionmentioning

confidence: 99%

“…Injection of excitotoxins, such as quinolinic, kainic or ibotenic acid, into the brain causes marked gliosis and severe inflammation around the injection site (Isacson et al, 1987;Dusart et al, 1991;Jørgensen et al, 1993;Marty et al, 1991;Nakao et al, 1996;Rostaing-Rigattieri et al, 1997). The objective of the present study was to assess whether an intense striatal inflammation induced by quinolinic acid (QUIN) injection would lead to rejection of neural allografts grafted into the striatum.…”

Section: Introductionmentioning

confidence: 99%

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Quinolinic acid‐induced inflammation in the striatum does not impair the survival of neural allografts in the rat

Duan

Widner

Cameron

et al. 1998

Eur J of Neuroscience

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It has been suggested that inflammation related to intracerebral transplantation surgery can affect the survival of intrastriatal neural allografts. To test this hypothesis, we transplanted dissociated embryonic mesencephalic tissue from one of two rat strains, Lewis (allogeneic grafts) or Sprague-Dawley (syngeneic grafts), to the striatum of Sprague-Dawley rats. The target striatum was either intact or had received a local injection of quinolinic acid 9 days earlier, in order to induce a marked inflammation. At 6 or 12 weeks after transplantation, there was no significant difference between the different groups regarding the number of surviving grafted tyrosine hydroxylase immunoreactive neurons. However, the graft volume of both the syngeneic and allogeneic implants was significantly larger in the quinolinate-lesioned than in the intact striatum. There were dramatically increased levels of expression of major histocompatibility complex class I and II antigens, marked infiltrates of macrophages, activated microglia and astrocytes, and accumulation of large numbers of CD4 and CD8 positive T-lymphocytes in the quinolinate-lesioned striatum. In contrast, these immunological markers were much less abundant around both syngeneic and allogeneic grafts placed in intact striatum. We conclude that severe inflammation caused by quinolinic acid does not lead to rejection of intrastriatal neural allografts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quinolinic acid‐induced inflammation in the striatum does not impair the survival of neural allografts in the rat

Duan

Widner

Cameron

et al. 1998

Eur J of Neuroscience

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Microglia in Cell Culture and in Transplantation Therapy for Central Nervous System Disease

Dobrenis

1998

Methods

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Different mechanisms mediate the rejection of porcine neurons and endothelial cells transplanted into the rat brain

Remy

Canova

Daguin‐Nerrière

et al. 2001

Xenotransplantation

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In order to investigate the early cellular responses mediating xenograft rejection in the brain, porcine aortic endothelial cells (PAEC) or porcine fetal mesencephalic neurons (PNEU) were transplanted into the striatum of LEW.1A rats. PAEC were detected with a specific anti-beta1 integrin antibody, and PNEU with an anti-porcine neurofilament antibody, or an antibody recognizing the NeuN antigen. PAEC grafts were massively infiltrated within 24 h by OX42-positive cells, which may correspond to polymorphonuclear (PMN) cells or macrophages. At that moment, the graft contained numerous cells expressing the inducible isoform of NO-synthase (iNOS). Infiltration by ED1-positive macrophages was effective after three days. The beta1-integrin labeling decreased from that time-point to day 7 post-implantation, and vanished after 11 days. Although some OX8-positive cells were present around the graft as soon as 3 days after transplantation, cells expressing the T-cell receptor (TCR)-beta chain infiltrated the graft after 7 days and their number remained low. A strong, diffuse OX8-and ED1-positive immunoreactive material remained in the scar up to the third week. In striking contrast, PNEU grafts remained poorly infiltrated by OX42- or ED1-positive cells during the first two weeks. A massive infiltration by macrophages and TCRbeta-positive lymphocytes occurred after 3 weeks. Natural killer (NK) cells were more scarce. The inflammation territory enlarged, and blood vessels were overloaded with macrophages or lymphocytes. Nevertheless, the graft contained NeuN-positive nuclei and neurites harbouring the porcine neurofilament protein. Hence, rejection was not completed at this time-point. These results suggest that the rapid rejection of PAEC is mainly driven by macrophages and possibly PMN cells, unlike PNEU, whose rejection is delayed and also involves lymphocytes. Differences in immunogenicity of grafted cells and/or patterns of production of pro-inflammatory cytokines may account for these contrasted rejection kinetics.

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Glial and endothelial cell response to a fetal transplant of purified neurons

Cited by 5 publications

References 57 publications

Quinolinic acid‐induced inflammation in the striatum does not impair the survival of neural allografts in the rat

Quinolinic acid‐induced inflammation in the striatum does not impair the survival of neural allografts in the rat

Microglia in Cell Culture and in Transplantation Therapy for Central Nervous System Disease

Different mechanisms mediate the rejection of porcine neurons and endothelial cells transplanted into the rat brain

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