Glass formation in plant anhydrobiotes: survival in the dry state

Buitink, Julia; Leprince, Olivier

doi:10.1016/j.cryobiol.2004.02.011

Cited by 302 publications

(221 citation statements)

References 74 publications

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“…Como se sabe, esses processos são profundamente influenciados pela umidade e pela temperatura. De acordo com Buitink & Leprince (2004), a identificação de cada componente citoplasmático que poderia contribuir para o estado vítreo perfeito nos sistemas biológicos é um dos grandes desafios científicos nessa área do conhecimento. Contudo, somente a formação do estado vítreo não é suficiente para manter a estabilidade celular e conseqüentemente a longevidade de sementes ortodoxas no estado desidratado.…”

Section: E Referências Contidas)unclassified

Variações dos carboidratos de reserva de sementes de Caesalpinia echinata (pau-brasil) armazenadas sob diferentes temperaturas

Hellmann

Mello

Barbedo

et al. 2008

Hoehnea

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-(Changes in the storage carbohydrates of Caesalpinia echinata (brazilwood) seeds stored under different temperatures). Among the major reserve carbohydrates of leguminous seeds are starch, sucrose and galactosyl oligosaccharides, contributing to structural stability of membranes during desiccation besides providing energy for embryo growth. Mature seeds of Caesalpinia echinata were harvested before and after shedding and reserve carbohydrates analyzed 15 and 90 days after storage at 25 ºC, 7 ºC and -18 ºC. The main compounds of the embryonic axis were soluble carbohydrates, especially sucrose, while starch represented ca 30% of the dry mass of the cotyledons. Recently dispersed seeds, with 12% of water content, had their carbohydrate contents unchanged and the germination capacity maintained when stored at cold temperatures (7 °C and -18 °C). However, mature seeds harvested before shedding, with 22% of water content, had their soluble carbohydrate contents decreased in the axis and starch increased in the cotyledons after 90 days of storage, but the viability was maintained only at 7 ºC. Chromatographic analysis showed that the hexose levels decreased during storage, except in the cotyledons of seeds stored at cold temperatures. Results suggest that the metabolism of C. echinata seeds was altered during storage but the fluctuations of non-structural carbohydrates during storage are not directly associated to the germination capacity of those seeds. Key words: cold temperature, Leguminosae, reserve compounds, starch, storage, sugars RESUMO -(Variações nos carboidratos de reserva de sementes de Caesalpinia echinata (pau-brasil) armazenadas sob diferentes temperaturas). Dentre os carboidratos de reserva de sementes destacam-se o amido, a sacarose e os oligossacarídeos galactosilados, que além da função de reserva, podem atuar na estabilidade das membranas durante a dessecação e a retomada do crescimento embrionário. Sementes maduras de Caesalpinia echinata Lam. foram coletadas antes e após a dispersão natural, sendo analisadas imediatamente após a coleta quanto à composição de carboidratos presentes no eixo embrionário e nos cotilédones, e aos 15 e 90 dias após armazenamento a 25 ºC, 7 ºC e -18 ºC. A principal reserva dos eixos embrionários foram os açúcares solúveis, enquanto que o amido foi a dos cotilédones, representando cerca de 30% da massa seca. Sementes recém-dispersas, com 12% de água, armazenadas sob temperaturas baixas (7 °C e -18 °C), mantiveram estável o teor de carboidratos totais nos cotilédones, bem como a capacidade germinativa, mas apresentaram redução no teor de açúcares solúveis. Já as sementes maduras não-dispersas, com 22% de água, mantiveram a capacidade germinativa apenas quando mantidas a 7 ºC. Nestas sementes, os açúcares solúveis diminuíram no eixo embrionário e nos cotilédones durante o armazenamento. Análises cromatográficas mostraram que, de modo geral, houve redução nos níveis de hexoses, exceto nos cotilédones de sementes armazenadas sob temperaturas baixas. As variações en...

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Section: E Referências Contidas)unclassified

Variações dos carboidratos de reserva de sementes de Caesalpinia echinata (pau-brasil) armazenadas sob diferentes temperaturas

Hellmann

Mello

Barbedo

et al. 2008

Hoehnea

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“…Mechanisms for longevity in the dry state provide stabilization of the biological entity for long periods of time by slowing down deteriorative reactions (Buitink and Leprince, 2004;Chatelain et al, 2012). As discussed above, Chl degradation and changes in the proportion of arabinose in the cell wall are highly correlated with longevity in resurrection plants and seeds.…”

Section: Mechanisms For Longevity In the Dry Statementioning

confidence: 94%

“…LEA proteins contribute to the stability of intracellular glasses and, therefore, to DT and survival in the dry state (Buitink and Leprince, 2004;Popova et al, 2015). LEA protein levels in maturing Arabidopsis and M. truncatula seeds are positively correlated with an increase in seed longevity (Hundertmark et al, 2011;Chatelain et al, 2012).…”

Section: Mechanisms For Longevity In the Dry Statementioning

confidence: 99%

“…The increase in solute concentration and the consequent increase in cytoplasmic viscosity are due to the accumulation of sugars, proteins, salts, organic acids, and amino acids. These compounds interact to form stable intracellular glasses, which ensure the optimal preservation of cellular components, proteins, and macromolecules in the dry state (Buitink and Leprince, 2004;Walters, 2015;Leprince et al, 2017).…”

Section: Maintenance Of Cell Integrity Via the Accumulation Of (Solidmentioning

confidence: 99%

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Orthodox Seeds and Resurrection Plants: Two of a Kind?

Costa

Cooper

Hilhorst³

et al. 2017

Plant Physiol.

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“…This state seems always to be characterized by the cessation of measurable metabolism. From this point, seed viability could be maintained during long periods owing to their glass structure, which was defined by Buitink & Leprince (2004) as a thermodynamically unstable state, with high viscosity (enabled by low tissue moisture and low temperature), so as the viscosity is so high that diffusional movement is effectively prevented for time periods or practical utility. Sun (2000); Walters (2007); Buitink & Leprince (2008) ascertained that glass stability is not upheld per se, it is based on groups of different biomolecules, linked by hydrogen bonds with water molecules.…”

Section: The Thermodynamics Of Seed and The Maintenance Of Seed Viabimentioning

confidence: 99%