Glandular trichome specificity of menthol biosynthesis pathway gene promoters from Mentha × piperita

Qamar, Nida; Pandey, Mytrai; Vasudevan, Madavan; Kumar, Ashish; Shasany, Ajit Kumar

doi:10.1007/s00425-022-04029-4

Cited by 4 publications

(4 citation statements)

References 69 publications

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“…Glandular trichomes have attracted increasing attention because of their ability to synthesize and secrete various plant substances. In many plants, such as A. annua and Mentha × piperita , glandular trichomes can synthesize artemisinin (Salehi et al, 2018; Zhou et al, 2020) and menthol mint (Qamar et al, 2022; Sharma et al, 2003). We have demonstrated through various methods that the CGT is the terpene synthesis and secretion site.…”

Section: Discussionmentioning

confidence: 99%

“…This is consistent with the changes in ROS content in leaves under UV-B, which suggested that CbMYB108 can improve the tolerance of C. blinii to UV-B stress. trichomes can synthesize artemisinin (Salehi et al, 2018;Zhou et al, 2020) and menthol mint (Qamar et al, 2022;Sharma et al, 2003). We have demonstrated through various methods that the CGT is the terpene synthesis and secretion site.…”

Section: Cbmyb108 Promotes the Expression Of Cbdxs And Cbggpps To Inc...mentioning

confidence: 99%

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CbMYB108 integrates the regulation of diterpene biosynthesis and trichome development in Conyza blinii against UV‐B

Zhan,

Di,

Chen

et al. 2024

Plant Cell & Environment

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Plants synthesize abundant terpenes through glandular trichomes (GTs), thereby protecting themselves from environmental stresses and increasing the economic value in some medicinal plants. However, the potential mechanisms for simultaneously regulating terpenes synthesis and GTs development remain unclear. Here, we showed that terpenes in Conyza blinii could be synthesized through capitate GTs. By treating with appropriate intensity of UV‐B, the density of capitate GTs and diterpene content can be increased. Through analyzing corresponding transcriptome, we identified a MYB transcription factor CbMYB108 as a positive regulator of both diterpene synthesis and capitate GT density. Transiently overexpressing/silencing CbMYB108 on C. blinii leaves could increase diterpene synthesis and capitate GT density. Further verification showed that CbMYB108 upregulated CbDXS and CbGGPPS expression in diterpene synthesis pathway. Moreover, CbMYB108 could also upregulated the expression of CbTTG1, key WD40 protein confirmed in this study to promote GT development, rather than through interaction between CbMYB108 and CbTTG1 proteins. Thus, results showed that the UV‐B‐induced CbMYB108 owned dual‐function of simultaneously improving diterpene synthesis and GT development. Our research lays a theoretical foundation for cultivating C. blinii with high terpene content, and broadens the understanding of the integrated mechanism on terpene synthesis and GT development in plants.

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Section: Discussionmentioning

confidence: 99%

Section: Cbmyb108 Promotes the Expression Of Cbdxs And Cbggpps To Inc...mentioning

confidence: 99%

CbMYB108 integrates the regulation of diterpene biosynthesis and trichome development in Conyza blinii against UV‐B

Zhan,

Di,

Chen

et al. 2024

Plant Cell & Environment

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“…Plant GTs, specialized epidermal cells synthesizing unique chemicals, hold significant scientific and practical significance ( Zakaria et al., 2019 ). Extensive research has been conducted on GTs to understand their capacity for secreting phytochemicals, such as peppermint ( Qamar et al., 2022 ), tomato ( Kortbeek et al., 2016 ), sweet wormwood ( Xiao et al., 2016 ), and tobacco ( Wagner, 2004 ). However, our understanding of GT development is poor ( Feng et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…This phenomenon is evident in prickly ash fruits ( Zanthoxylum armatum ; Zanthoxylum bungeanum ) ( Fei et al., 2021 ) and passion fruit ( Passiflra edulis ) ( Li et al., 2021 ). In tobacco ( Nicotiana tabacum ) leaves, nicotine is mainly secreted by GT and increases with leaf development ( Qamar et al., 2022 ). In Cinnamomum cassia , the essential oil accumulation coincides with leaf development ( Li et al., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

A comparative analysis of morphology, microstructure, and volatile metabolomics of leaves at varied developmental stages in Ainaxiang (Blumea balsamifera (Linn.) DC.)

Chen,

Li,

Pang

et al. 2023

Front. Plant Sci.

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IntroductionAinaxiang (Blumea balsamifera (Linn.) DC.) is cultivated for the extraction of (-)-borneol and other pharmaceutical raw materials due to its abundant volatile oil. However, there is limited knowledge regarding the structural basis and composition of volatile oil accumulation in fresh B. balsamifera leaves.MethodsTo address this problem, we compare the fresh leaves’ morphology, microstructure, and volatile metabonomic at different development stages, orderly defined from the recently unfolded young stage (S1) to the senescent stage (S4).Results and discussionDistinct differences were observed in the macro-appearance and microstructure at each stage, particularly in the B. balsamifera glandular trichomes (BbGTs) distribution. This specialized structure may be responsible for the accumulation of volatile matter. 213 metabolites were identified through metabolomic analysis, which exhibited spatiotemporal accumulation patterns among different stages. Notably, (-)-borneol was enriched at S1, while 10 key odor metabolites associated with the characteristic balsamic, borneol, fresh, and camphor aromas of B. balsamifera were enriched in S1 and S2. Ultra-microstructural examination revealed the involvement of chloroplasts, mitochondria, endoplasmic reticulum, and vacuoles in the synthesizing, transporting, and storing essential oils. These findings confirm that BbGTs serve as the secretory structures in B. balsamifera, with the population and morphology of BbGTs potentially serving as biomarkers for (-)-borneol accumulation. Overall, young B. balsamifera leaves with dense BbGTs represent a rich (-)-borneol source, while mesophyll cells contribute to volatile oil accumulation. These findings reveal the essential oil accumulation characteristics in B. balsamifera, providing a foundation for further understanding.

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Effects of MhMYB1 and MhMYB2 transcription factors on the monoterpenoid biosynthesis pathway in l-menthol chemotype of Mentha haplocalyx Briq

An,

Liao,

et al. 2024

Planta

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Glandular trichome specificity of menthol biosynthesis pathway gene promoters from Mentha × piperita

Cited by 4 publications

References 69 publications

CbMYB108 integrates the regulation of diterpene biosynthesis and trichome development in Conyza blinii against UV‐B

CbMYB108 integrates the regulation of diterpene biosynthesis and trichome development in Conyza blinii against UV‐B

A comparative analysis of morphology, microstructure, and volatile metabolomics of leaves at varied developmental stages in Ainaxiang (Blumea balsamifera (Linn.) DC.)

Effects of MhMYB1 and MhMYB2 transcription factors on the monoterpenoid biosynthesis pathway in l-menthol chemotype of Mentha haplocalyx Briq

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