CbMYB108 integrates the regulation of diterpene biosynthesis and trichome development in Conyza blinii against UV‐B

Abstract

Plants synthesize abundant terpenes through glandular trichomes (GTs), thereby protecting themselves from environmental stresses and increasing the economic value in some medicinal plants. However, the potential mechanisms for simultaneously regulating terpenes synthesis and GTs development remain unclear. Here, we showed that terpenes in Conyza blinii could be synthesized through capitate GTs. By treating with appropriate intensity of UV‐B, the density of capitate GTs and diterpene content can be increased. Through analyzing corresponding transcriptome, we identified a MYB transcription factor CbMYB108 as a positive regulator of both diterpene synthesis and capitate GT density. Transiently overexpressing/silencing CbMYB108 on C. blinii leaves could increase diterpene synthesis and capitate GT density. Further verification showed that CbMYB108 upregulated CbDXS and CbGGPPS expression in diterpene synthesis pathway. Moreover, CbMYB108 could also upregulated the expression of CbTTG1, key WD40 protein confirmed in this study to promote GT development, rather than through interaction between CbMYB108 and CbTTG1 proteins. Thus, results showed that the UV‐B‐induced CbMYB108 owned dual‐function of simultaneously improving diterpene synthesis and GT development. Our research lays a theoretical foundation for cultivating C. blinii with high terpene content, and broadens the understanding of the integrated mechanism on terpene synthesis and GT development in plants.

GmMYB114 Facilitates the Synthesis of Anthocyanins in Soybean Sprouts under Blue Light

“…Plasmids of pCAMBIA1305.1-GFP, pCAMBIA1305.1-GmMYB114-GFP, and a nuclear marker (MADS3-RFP) were, respectively, transferred into Agrobacterium strain GV3101 (Qingke, Beijing, China). The transient expression of vectors in Nicotiana benthamiana seedlings was performed according to the previously mentioned protocol [ 45 ]. Three days after infiltration, the undamaged epidermis of the leaves injected with a bacterial solution was selected for observation.…”

“…For GUS analysis, following the procedure outlined by Zhan [ 45 ], the promoters were incorporated into the PCXGUS vector and then transferred into Agrobacterium rhizogenes K599. The A. rhizogenes strain K599 carrying both the effector and reporter was intercombined and transformed into soybean hairy roots.…”