Glandular hairs and essential oils in micropropagated plants of Salvia officinalis L.

Avato, Pinarosa; Fortunato, I. Morone; Ruta, C.; D’Elia, Roberta

doi:10.1016/j.plantsci.2005.02.004

Cited by 88 publications

(75 citation statements)

References 17 publications

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“…This chemical profile agrees qualitatively with the compounds reported by different authors for S. officinalis oils (SerAFINI; BArroS; AzeVeDo, 2001;VeLICKoVIC et al, 2002;AVATo et al, 2005;DeLAMAre et al, 2007). SAYeD et al (2001) found1,8-cineol as the major component of Salvia triloba (concentration higher than 60%) and smaller amounts of α and β-thujone.…”

Section: Sample Analysessupporting

confidence: 91%

“…The Petri dishes were incubated at 36 °C for 24 hours. The mean diameter of popular medicine and many pharmacological studies intended to identify the compounds responsible for their therapeutic effects (KIM;MArSHALL;WeI, 1995;eVANS, 2002;SerAFINI et al, 2002;rADULeSCU;CHILIMeNT;oPreA, 2004;AVATo et al, 2005).…”

Section: Disk Diffusion Methodsmentioning

confidence: 99%

“…essentials oils are in fact complex mixtures of volatile substances, insoluble in water and soluble in organic solvents, which contribute to their characterization and isolation. They may contain a mixture of terpenes, monoterpenes, sesquiterpenes, or even diterpenes, low molecular weight aliphatic hydrocarbons (linear, branched, saturated and unsaturated), acids, alcohols, aldehydes, acyclic esters or lactones and exceptionally nitrogen and sulphur compounds, cumarines, and homologous of phenylpropanes (VeLICKoVIC et al, 2002;AVATo et al, 2005). essential oils extracted from vegetable matrices have been considered as growth inhibitors of food and human pathogens such as Escherichia coli, Staphylococcus sp., Bacillus sp., Streptococcus sp., Salmonella sp., Mycobacterium sp., Vibrio vulnificus, among others.…”

Section: Introductionmentioning

confidence: 99%

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Chemical characterization and antimicrobial activity of essential oils of salvia L. species

Pierozan¹,

Pauletti²,

Rota³

et al. 2009

Ciênc. Tecnol. Aliment.

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Section: Sample Analysessupporting

confidence: 91%

Section: Disk Diffusion Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemical characterization and antimicrobial activity of essential oils of salvia L. species

Pierozan¹,

Pauletti²,

Rota³

et al. 2009

Ciênc. Tecnol. Aliment.

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“…R. Zuzarte et al (2010) during morphological analysis demonstrated that trichomes on Lavandula pedunculata leaves in vitro are similar to the ones in intact plants. Histological investigation by Avato P. (2005) and Kamatou G.P.P. (2007) over Salvia officinalis, S. albicaulis and S. dolomitica plants on different stages of aseptic culture demonstrated the presence of shield-shaped glandular scales that produced high quality essential oils.…”

Section: Resultsmentioning

confidence: 98%

Anatomy Features of Lavandula Angustifolia Mill. and Lavandula Hybrida Rev. Plants in Vitro

Brailko¹,

Митрофанова²,

Лесникова-Седошенко³

et al. 2017

AgricultForest

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SUMMARYLavender and lavandin are widely used in food, pharmaceutical, cosmetic industries and ornamental gardening. Gene pool collection of Lavandula angustifolia and lavandin is presented in Nikita Botanical Gardens plots and the same collection is being created in vitro.Initial explants of L. angustifolia, its cultivars 'Belyanka', 'Record' and L. hybrida -'Rabat' and 'Snezhnyi Bars' were introduced in vitro. Plant regeneration occurred on MS medium supplemented with 0.3-0.5 mg l -1 kinetin, 0.025 mg l -1 NAA, 0.25 mg l -1 GA 3 . Samples were analyzed after 2 and 8 months of culture.Structural analysis of regenerated plants vegetative organs enabled assessment of their morphogenetic capacity. Under in vitro culture 2-5 microshoots/explants were produced with the height of 2.3 -8.2 cm. Leaves were opposite, sessile linear or linear-lanceolate, with entire edges curved outward, densely pubescent, and 10-30 leaves/microshoot. Leaf lamina length was 0.9-1.5 cm. Leaves were bifacial, amphystomatic, lamina thickness -132-215 µm, palisade rate was 0.38-0.41 after 2 months culture and 0.56-0.58 -after 8 months. Epidermal thickness was 8-19 µm, cells were relatively small with thin, cellulose walls covered with cuticle. Stomata are of anomocyte type, small, raised above the epiderm cells, 48-150 pcs/mm 2 on adaxial surface, 115-432 pcs/mm 2 -on abaxial one. Essential oils are produced in simple and glandular, unicellular and multicellular hairs of epidermal origin, located above the leaf chlorophyll tissue. Features of xeromorphic structure appeared at a long-term in vitro cultivation.

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“…Pregerminated seedlings of each variety of alfalfa were aseptically transferred to 500 ml jars (20 per jar) containing a basal medium -BM [51] enriched with different concentrations of sodium chloride (0, 50, 100, 150, 200 mM NaCl). The medium was sterilized by autoclaving for 20 minutes at 121°C.…”

Section: Salt Treatmentsmentioning

confidence: 99%

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

et al. 2013

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In order to quickly and efficiently evaluate the salt tolerance of alfalfa, salinity tests were conducted on Medicago sativa L. var. australis, var. icon, var. loi, and var. gea, under in vitro conditions. Pregerminated seeds of four varieties were subjected to five different NaCl concentrations (0, 50, 100, 150, 200 mM). The influence of saline stress was estimated on the basis of survival percentage, growth parameters, and electrolyte leakage. The seedlings surviving on the medium enriched with salt at the highest concentration were presumed to be tolerant and represented the mother plants for the production of in vitro clones. In the following step, the clones were evaluated in vitro to confirm the salt tolerance. The influence of mild salt stress (75 mM NaCl) on the growth parameters of selected clones was examined. At the end of this trial, the proline accumulation and sodium content in alfalfa shoots were also quantified. The results suggest an increased level of proline promotes salt tolerance. Medicago sativa L. var. icon is highly tolerant in comparison with the other varieties tested. In vitro selection of M. sativa L. varieties on salt-containing media allowed us to obtain clones with increased salinity tolerance.

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Glandular hairs and essential oils in micropropagated plants of Salvia officinalis L.

Cited by 88 publications

References 17 publications

Chemical characterization and antimicrobial activity of essential oils of salvia L. species

Chemical characterization and antimicrobial activity of essential oils of salvia L. species

Anatomy Features of Lavandula Angustifolia Mill. and Lavandula Hybrida Rev. Plants in Vitro

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

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