Glaciecola nitratireducens sp. nov., isolated from seawater

Abstract

A marine bacterial strain, FR1064 T , was isolated from a surface seawater sample collected off Jeju Island, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the Gammaproteobacteria and was related to the genus Glaciecola with 97?6 % sequence similarity to Glaciecola pallidula, its nearest phylogenetic neighbour. DNA-DNA relatedness between strain FR1064 T and G. pallidula ACAM 615 T was 55 %. Cells of the novel isolate were Gram-negative, aerobic, rod-shaped, motile and halophilic, with an optimum sea salts concentration of 4-7 %. The major fatty acids were straight-chain saturated (C 16 : 0 ), summed feature 3 and monounsaturated fatty acid C 18 : 1 . The DNA G+C content was 44 mol%. Several phenotypic characteristics differentiated the novel isolate from all previously described members of the genus Glaciecola. The polyphasic data obtained in this study clearly demonstrate that strain FR1064 T represents a novel species of the genus Glaciecola. The name Glaciecola nitratireducens sp. nov. is therefore proposed, with strain FR1064 T (=KCTC 12276 T =JCM 12485 T ) as the type strain.The genus Glaciecola was originally created to accommodate aerobic, psychrophilic, halophilic bacteria and initially comprised two species, Glaciecola punicea and Glaciecola pallidula. These species were isolated from sea-ice diatom assemblage samples collected from coastal areas of eastern Antarctica (Bowman et al., 1998). Recently, two further species of the genus, namely Glaciecola mesophila and Glaciecola polaris, have been described, isolated from marine invertebrate specimens and Arctic Ocean seawater, respectively (Romanenko et al., 2003;Van Trappen et al., 2004). In the course of our study on marine microbial diversity, a Glaciecola-like strain, designated strain FR1064 T , was isolated from surface seawater and was the subject of a comprehensive taxonomic investigation. In this study, the polyphasic taxonomic properties of strain FR1064 T are presented.Strain FR1064 T was isolated from a coastal surface seawater sample collected off Jeju Island, Republic of Korea. The sample was diluted with sterilized artificial seawater (ASW; Lyman & Fleming, 1940), spread onto a plate containing marine agar 2216 (MA; Difco) and incubated at 25 u C for 3 weeks. The isolate was routinely cultured on MA and maintained as a glycerol suspension (20 %, w/v) at 280 uC. G. pallidula ACAM 615 T , cultured on MA at 15 u C, was used as a reference strain.Bacterial DNA preparation, PCR amplification and sequencing of the 16S rRNA gene were carried out as described previously (Chun & Goodfellow, 1995). The resulting gene sequence of strain FR1064 T was aligned manually against sequences obtained from GenBank. Phylogenetic trees were inferred from the regions available for all sequences (positions 28-1433; Escherichia coli numbering system) using the Fitch-Margoliash (Fitch & Margoliash, 1967), maximum-likelihood (Felsenstein, 1981), maximum-parsimony (Fitch, 1971) and neighbour-joining (Saitou ...

Aliagarivorans marinus gen. nov., sp. nov. and Aliagarivorans taiwanensis sp. nov., facultatively anaerobic marine bacteria capable of agar degradation

“…The remaining isoprenoid quinones included RQ-10 (5.7 % for AAM1 T and 15.6 % for AAT1 T ), Q-9 (1.8 % for AAM1 T and 2.0 % for AAT1 T ) and MK-8 (0.5 % for AAM1 T and 2.6 % for AAT1 T ). They had DNA G+C contents of 52.4-52.9 mol%, which were greater than those of Agarivorans albus (48.7-49.7 mol%; Kurahashi & Yokota, 2004), Alteromonas species (43.0-46.4 mol%; Bowman & McMeekin, 2005;Chiu et al, 2007;Ivanova et al, 2005;Martínez-Checa et al, 2005;Van Trappen et al, 2004a;Yoon et al, 2003Yoon et al, , 2004, Glaciecola species (40.0-45.0 mol%; Baik et al, 2006;Bowman et al, 1998;Matsuyama et al, 2006;Romanenko et al, 2003;Yong et al, 2007;Van Trappen et al, 2004b;Zhang et al, 2006), Aestuariibacter salexigens (48.0 mol%; Yi et al, 2004), S. chungwhensis (48.0 mol%; Jeon et al, 2005) and B. denitrificans (50.0 mol%; Jean et al, 2006c). Aestuariibacter halophilus was the only species in the Alteromonadaceae that had a DNA G+C content that was greater (54.0 mol%; Yi et al, 2004) than those of our isolates.…”

“…Extraction and purification of total genomic DNA from the cells and PCR amplification of 16S rRNA genes were performed according to previously described methods (Jean et al, 2006b). Sequencing of the 16S rRNA genes, alignment and comparison of the resulting sequences with reference sequences available in GenBank, calculation of distance matrices for the aligned sequences and reconstruction of phylogenetic trees based on the neighbour-joining, maximum-parsimony and maximum-likelihood methods were performed as described by Shieh et al (2004) and Jean et al (2006b Kurahashi & Yokota, 2004), eight Alteromonas species (84.9-87.1 %; Bowman & McMeekin, 2005;Chiu et al, 2007;Ivanova et al, 2005;Martínez-Checa et al, 2005;Van Trappen et al, 2004a;Yoon et al, 2003Yoon et al, , 2004, two Aestuariibacter species (86.0-87.0 %; Yi et al, 2004), Bowmanella denitrificans (86.1-86.7 %; Jean et al, 2006c), eight Glaciecola species (85.0-87.9 %; Baik et al, 2006;Bowman et al, 1998;Matsuyama et al, 2006;Romanenko et al, 2003;Yong et al, 2007;Van Trappen et al, 2004b;Zhang et al, 2006) and Salinimonas chungwhensis (85.9-86.1 %; Jeon et al, 2005). The distant relationship between the two novel isolates and these bacteria was also evident in the neighbour-joining tree, in which the two isolates formed a stable monophyletic clade (bootstrap value, 100 %) located next to a sister clade comprising only Agarivorans albus strains (Fig.…”

Lacimicrobium alkaliphilum gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from a salt lake

“…Furthermore, strain X13M-12 T could be distinguished from the type strains of the type species of the genera Aestuariibacter, Salinimonas, Agaribacter, Aliiglaciecola, Glaciecola, Paraglaciecola and Alteromonas by the presence of C 17 : 0 cyclo and C 19 : 0 10-methyl, and due to the lack of C 15 : 0 . (Teramoto & Nishijima, 2014); 6, Aliiglaciecola (Chen et al, 2009a;Jean et al, 2013;Jin et al, 2015;Tanaka et al, 2010); 7, Glaciecola (Baik et al, 2006;Bowman et al, 1998); 8, 'Pseudobowmanella' (Du et al, 2015); 9, Paraglaciecola (Matsuyama et al, 2006;Park & Yoon, 2013;Romanenko et al, 2003;Shivaji & Reddy, 2014;Van Trappen et al, 2004a;Yong et al, 2007;Zhang et al, 2006;Zhang et al, 2011); 10, Alteromonas (Baumann et al, 1972;Chen et al, 2009b;Chiu et al, 2007;Ivanova et al, 2005;Ivanova et al, 2013;Ivanova et al, 2015;Martínez-Checa et al, 2005;Matsuyama et al, 2015;Van Trappen et al, 2004b;Vandecandelaere et al, 2008;Yi et al, 2004;Yoon et al, 2003;Yoon et al, 2004) Oxygen requirements…”

Aurantimonas frigidaquae sp. nov., isolated from a water-cooling system

“…Genomic relatedness was determined using a membrane filter technique (Seldin & Dubnau, 1985) according to the method described by Baik et al (2006). DNA relatedness of strain CW5 T with A. altamirensis KACC 11704 T and A. coralicida KACC 12050 T was 45 and 28 %, respectively.…”