Glabridin inhibits urothelial bladder carcinoma cell growth in vitro and in vivo by inducing cell apoptosis and cell cycle arrest

Abstract

Glabridin (GLA) has a variety of biological activities and therapeutic effects in cancers. Whereas the effect of GLA on urothelial bladder carcinoma (UBC) cells and its underlying mechanisms remain unknown. The study revealed the effect of GLA on UBC and the potential mechanism of inducing cell apoptosis in vivo and in vitro. After treated with different concentrations of GLA, the cell activity decreased in a time‐ and dose‐dependent manner. The IC50 values of BIU‐87 and EJ cells at 48 h were 6.02 μg/ml (18.6 μm) and 4.36 μg/ml (13.4 μm), respectively. Additionally, GLA‐induced apoptosis and cycle arrest of BIU‐87 and EJ cells in G2 phase. Furthermore, wound healing experiments showed that GLA significantly reduced the migration activities of BIU‐87 and EJ cells. Mechanically, GLA obviously increased the expression of BIM, BAK1, and CYCS in both mRNA and protein levels, which led to the activation of the endogenous apoptotic pathway. Finally, GLA remarkably inhibited the growth of UBC tumors in vivo. In summary, GLA inhibited UBC cells growth in vitro and in vivo by inducing cell apoptosis and cell cycle arrest, highlighting that GLA could be utilized as a component to design a novel anti‐UBC drug.

Peimenine unleashes therapeutic promise in urothelial bladder cancer: inhibition of proliferation, induction of cell death and modulation of key pathways

“…After processing the cells as mentioned earlier (Yang, Bi, et al., 2023), the total protein content was assessed using a BCA reagent (PC0020, Solarbio, China). SDS‐PAGE was utilized to separate the proteins, followed by their transfer onto a PVDF membrane (Millipore, USA).…”

“…After processing the cells as mentioned earlier (Yang, Bi, et al, 2023), the total protein content was assessed using a BCA reagent (PC0020, Solarbio, China). SDS-PAGE was Cell vitality = (OD of the experimental group)∕ (OD of the control group) × 100%.…”

Medicarpin suppresses proliferation and triggeres apoptosis by upregulation of BID, BAX, CASP3, CASP8, and CYCS in glioblastoma

“…The results showed that the PTX delivery system with curcumin derivatives as carrier had a strong killing effect on tumor cells rather than normal cells. Notably, our research group also found that glabridin has outstanding anti‐bladder cancer properties (IC 50 = 13.4 μM) (Yang et al, 2023), ligustilide has anti‐bladder cancer ability (IC 50 = 209.8 μM) (Yin et al, 2023), and paeonol (PAE) has certain anti‐glioma effect (IC 50 = 527.29 μM) (Hao et al, 2023).…”

Mechanistic inhibition of gastric cancer-associated bacteria Helicobacter pylori by selected phytocompounds: A new cutting-edge computational approach

“…Glabridin, a prenylated isoflavone found in the roots of G. glabra L. (European licorice, Fabaceae), has a wide variety of biological effects, including antioxidant, anti-inflammatory, anti-atherogenic, regulation of energy metabolism, estrogenic, neuroprotective, anti-osteoporotic, and skin-whitening effects [ 24 ]. Glabridin has many biological functions and therapeutic benefits in cancer treatment [ 25 ], including anti-inflammatory and antioxidant properties. The pharmacological effects of Glabridin are numerous and include the prevention of Staphylococcus, Candida , and other bacterial infections, the treatment of metabolic abnormalities to reduce obesity, diabetes, and cardiovascular disease, the protection of nervous system function, the prevention of cancer, the treatment of inflammation, the treatment of osteoporosis, etc.…”