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Zhang, Zhanyuan; Xing, Aiqiu; Staswick, Paul E.; Clemente, Thomas E.

doi:10.1023/a:1006298622969

Cited by 180 publications

(29 citation statements)

References 26 publications

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“…Soybean cultivar Maverick was transformed by Agrobacterium -cotyledonary node method (Hinchee et al, 1988). Regenerated transgenic soybean plants were screened for tolerance to herbicide Liberty by a leaf-painting assay as described earlier (Zhang et al, 1999). The knockout of the target proteins in glufosinate-resistance plants was confirmed by PCR and SDS-PAGE.…”

Section: Methodsmentioning

confidence: 99%

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

2014

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Expression of heterologous methionine-rich proteins to increase the overall sulfur amino acid content of soybean seeds has been only marginally successful, presumably due to low accumulation of transgenes in soybeans or due to gene silencing. Proteome rebalancing of seed proteins has been shown to promote the accumulation of foreign proteins. In this study, we have utilized RNAi technology to suppress the expression of the β-conglycinin, the abundant 7S seed storage proteins of soybean. Western blot and 2D-gel analysis revealed that β-conglycinin knockdown line (SAM) failed to accumulate the α′, α, and β-subunits of β-conglycinin. The proteome rebalanced SAM retained the overall protein and oil content similar to that of wild-type soybean. We also generated transgenic soybean lines expressing methionine-rich 11 kDa δ-zein under the control of either the glycinin or β-conglycinin promoter. The introgression of the 11 kDa δ-zein into β-conglycinin knockdown line did not enhance the accumulation of the 11 kDa δ-zein. However, when the same plants were grown in sulfur-rich medium, we observed 3- to 16-fold increased accumulation of the 11 kDa δ-zein. Transmission electron microscopy observation revealed that seeds grown in sulfur-rich medium contained numerous endoplasmic reticulum derived protein bodies. Our findings suggest that sulfur availability, not proteome rebalancing, is needed for high-level accumulation of heterologous methionine-rich proteins in soybean seeds.

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Section: Methodsmentioning

confidence: 99%

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

2014

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“…Of the different compounds tested, in either normal FNL or 1/5 normal FNL medium, Liberty ® (glufosinate) was found to be effective at the lowest concentration level 2.5 μg/mL. Using the cotyledonary node method of soybean transformation Zhang et al [42] observed 3-5 μg/ml glufosinate was sufficient to prevent shoot initiation. These amounts are similar to the concentrations of glufosinate required to inhibit SSE proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…However, herbicides such as glyphosate and glufosinate have been extensively used in the transformation and selection of several crops such as maize, tobacco, wheat, rice, alfalfa, etc. and soybeans with the cotyledonary node adventitious regeneration system [15]. Aragão, Rech and co-workers [16,17] report on use of a modified acetohydroxy acid synthase gene to bombard embryonic axes and select soybeans transformed by a cotyledonary node system with the ALS inhibiting imidazolinone herbicide, Imazapyr.…”

Section: Introductionmentioning

confidence: 99%

“…As a consequence, PPT acts as a glutamate analogue and inhibits glutamine synthetase leading to plant cell death [21-23]. The bar gene has been used as the selectable marker coupled with glufosinate as a selection agent with the Agrobacterium tumefaciens -cotyledonary node transformation system for soybeans [15] succeeded in germ-line transformation events at frequencies up to 3% using a selection regime of 5 mg/L glufosinate during the shoot initiation stage and 2 mg/L during shoot elongation with soybean cotyledonary nodes transformed with Agrobacterium . Simmonds and Donaldson [24] report on the selection of two transgenic soybean lines by selecting somatic embryo cultures expressing a phosphinothricin N-acetyltransferase gene with glufosinate.…”

Section: Introductionmentioning

confidence: 99%

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Non-antibiotic selection systems for soybean somatic embryos: the lysine analog aminoethyl-cysteine as a selection agent

et al. 2009

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BackgroundIn soybean somatic embryo transformation, the standard selection agent currently used is hygromycin. It may be preferable to avoid use of antibiotic resistance genes in foods. The objective of these experiments was to develop a selection system for producing transgenic soybean somatic embryos without the use of antibiotics such as hygromycin.ResultsWhen tested against different alternate selection agents our studies show that 0.16 μg/mL glufosinate, 40 mg/L isopropylamine-glyphosate, 0.5 mg/mL (S-(2 aminoethyl)-L-cysteine) (AEC) and the acetolactate synthase (ALS) inhibitors Exceed® and Synchrony® both at 150 μg/mL inhibited soybean somatic embryo growth. Even at the concentration of 2 mg/mL, lysine+threonine (LT) were poor selection agents. The use of AEC may be preferable since it is a natural compound. Unlike the plant enzyme, dihydrodipicolinate synthase (DHPS) from E. coli is not feed-back inhibited by physiological concentrations of lysine. The dapA gene which codes for E. coli DHPS was expressed in soybean somatic embryos under the control of the CaMV 35S promoter. Following introduction of the construct into embryogenic tissue of soybean, transgenic events were recovered by incubating the tissue in liquid medium containing AEC at a concentration of 5 mM. Only transgenic soybeans were able to grow at this concentration of AEC; no escapes were observed.ConclusionGenetically engineered soybeans expressing a lysine insensitive DHPS gene can be selected with the non-antibiotic selection agent AEC. We also report here the inhibitory effects of glufosinate, (isopropylamine-glyphosate) (Roundup®), AEC and the ALS inhibitors Exceed® and Synchrony® against different tissues of soybean

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“…47,50 Selection systems for both transformation methods include antibiotics, such as hygromycin, for research-related transformations and herbicide tolerance to glyphosate, glufosinate or sulfonureas for production transformations. [51][52][53] 8.6 Biotechnology Approaches to Improving SoybeanOil Quality…”

Section: Genetic Transformation Of Soybeanmentioning

confidence: 99%

Soybeans

Kinney

Clemente

2010

Energy Crops

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For most of their 300 year domesticated history soybeans have been grown to feed humans and animals. In the last decade there has been increasing use of soybean oil (which constitutes about 20% of the seed) for energy, in the form of methyl ester biodiesel. Soybean biodiesel, while more expensive to produce than petroleum diesel, offers a number of advantages over all petrochemical fuels, including increased fuel performance, lower carbon emissions and biodegradability. Soybean biodiesel use is limited by the functional properties of soybean oil, cost and oil supply. The oxidative instability of soybean oil will compromise ignition performance. Saturated fatty acids reduce the cold flow properties of soybean biodiesel and limit its use in cold environments. High oleic, low palmitic acid soybean oil addresses these functional limitations. Efforts by plant breeders over a 30 year period has resulted in high oleic, low palmitic acid soybean lines but they have not been commercialized due to the breeding challenges associated with pyramiding multigenic factors, required for full penetration of the target trait and the observed instability of the phenotype across environments, as well as poor agronomic performance of the crop. On the other hand, using the tools of biotechnology, single locus, environmentally stable, high oleic, low palmitic acid soybean lines have been produced in high performing elite varieties. These varieties are being commercialized by major seed companies. The methyl ester biodiesel from these lines has improved functional properties including cold flow characteristics similar to petroleum diesel. Future biotechnology efforts will be directed towards improving the oil yield of the soybean crop.

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Cited by 180 publications

References 26 publications

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

Non-antibiotic selection systems for soybean somatic embryos: the lysine analog aminoethyl-cysteine as a selection agent

Soybeans

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