GIP1 and GIP2 Contribute to the Maintenance of Genome Stability at the Nuclear Periphery

Singh, Gaurav; Batzenschlager, Morgane; Tomkova, Denisa; Herzog, Étienne; Hoffmann, Elise; Houlné, Guy; Schmit, Anne‐Catherine; Berr, Alexandre; Chabouté, Marie‐Edith

doi:10.3389/fpls.2021.804928

Cited by 4 publications

(3 citation statements)

References 55 publications

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“…The gip1 gip2 -mutant display enhanced resistance to hyperosmotic stress partially contributed by mechanical shielding through nuclear shrinking and stiffening [ 85 ]. GIP proteins have also been implicated in the maintenance of genome stability in Arabidopsis, where GIP proteins are predominantly colocalized with the homologous recombination (HR) protein RAD51 at the nuclear periphery [ 86 ]. Nuclei in the gip1 gip2 mutant exhibit abundant and heterogeneous γ-H2AX foci, suggesting constitutive DNA damage and chromosome instability.…”

Section: Inner Nuclear Membrane Contains Diverse Regulators Of Chroma...mentioning

confidence: 99%

Plant nuclear envelope as a hub connecting genome organization with regulation of gene expression

Tang¹

2023

Nucleus

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Eukaryotic cells organize their genome within the nucleus with a double-layered membrane structure termed the nuclear envelope (NE) as the physical barrier. The NE not only shields the nuclear genome but also spatially separates transcription from translation. Proteins of the NE including nucleoskeleton proteins, inner nuclear membrane proteins, and nuclear pore complexes have been implicated in interacting with underlying genome and chromatin regulators to establish a higher-order chromatin architecture. Here, I summarize recent advances in the knowledge of NE proteins that are involved in chromatin organization, gene regulation, and coordination of transcription and mRNA export. These studies support an emerging view of plant NE as a central hub that contributes to chromatin organization and gene expression in response to various cellular and environmental cues.

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Section: Inner Nuclear Membrane Contains Diverse Regulators Of Chroma...mentioning

confidence: 99%

Plant nuclear envelope as a hub connecting genome organization with regulation of gene expression

Tang¹

2023

Nucleus

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“…In Arabidopsis, crwn1crwn4 double mutants are hypersensitive to genotoxic stress and show reduced anchoring of RAD54 at the nuclear periphery (Hirakawa & Matsunaga, 2019). Similarly, gip1gip2 mutants present increased genome instability due to impaired recruitment of RAD51 at double‐stranded DNA breaks at the nuclear periphery of root meristematic nuclei (Singh, Batzenschlager, et al., 2021). Therefore, the NE in eukaryotes contributes to homologous recombination repair and genome stability at the nuclear periphery.…”

Section: Ne Dynamics and Its Functional Relevancementioning

confidence: 99%

Nuclear envelope dynamics in connection to chromatin remodeling

et al. 2023

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SUMMARYThe nucleus is a central organelle of eukaryotic cells undergoing dynamic structural changes during cellular fundamental processes such as proliferation and differentiation. These changes rely on the integration of developmental and stress signals at the nuclear envelope (NE), orchestrating responses at the nucleo‐cytoplasmic interface for efficient genomic functions such as DNA transcription, replication and repair. While in animals, correlation has already been established between NE dynamics and chromatin remodeling using last‐generation tools and cutting‐edge technologies, this topic is just emerging in plants, especially in response to mechanical cues. This review summarizes recent data obtained in this field with more emphasis on the mechanical stress response. It also highlights similarities/differences between animal and plant cells at multiples scales, from the structural organization of the nucleo‐cytoplasmic continuum to the functional impacts of NE dynamics.

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“…However, according to Singh et al, RAD51 did not co-localized with γH2AX. Moreover, the gamma-tubulin complex component 3-interacting protein 1 (GIP1), involved in the maintenance of the nuclear structure and organization, forms nuclear foci that co-localize with γH2AX foci ( Singh et al, 2022 ).…”

Section: Nuclear Bodiesmentioning

confidence: 99%

Nuclear dynamics: Formation of bodies and trafficking in plant nuclei

Muñoz-Díaz

Sáez‐Vasquez

2022

Front. Plant Sci.

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The existence of the nucleus distinguishes prokaryotes and eukaryotes. Apart from containing most of the genetic material, the nucleus possesses several nuclear bodies composed of protein and RNA molecules. The nucleus is separated from the cytoplasm by a double membrane, regulating the trafficking of molecules in- and outwards. Here, we investigate the composition and function of the different plant nuclear bodies and molecular clues involved in nuclear trafficking. The behavior of the nucleolus, Cajal bodies, dicing bodies, nuclear speckles, cyclophilin-containing bodies, photobodies and DNA damage foci is analyzed in response to different abiotic stresses. Furthermore, we research the literature to collect the different protein localization signals that rule nucleocytoplasmic trafficking. These signals include the different types of nuclear localization signals (NLSs) for nuclear import, and the nuclear export signals (NESs) for nuclear export. In contrast to these unidirectional-movement signals, the existence of nucleocytoplasmic shuttling signals (NSSs) allows bidirectional movement through the nuclear envelope. Likewise, nucleolar signals are also described, which mainly include the nucleolar localization signals (NoLSs) controlling nucleolar import. In contrast, few examples of nucleolar export signals, called nucleoplasmic localization signals (NpLSs) or nucleolar export signals (NoESs), have been reported. The existence of consensus sequences for these localization signals led to the generation of prediction tools, allowing the detection of these signals from an amino acid sequence. Additionally, the effect of high temperatures as well as different post-translational modifications in nuclear and nucleolar import and export is discussed.

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GIP1 and GIP2 Contribute to the Maintenance of Genome Stability at the Nuclear Periphery

Cited by 4 publications

References 55 publications

Plant nuclear envelope as a hub connecting genome organization with regulation of gene expression

Plant nuclear envelope as a hub connecting genome organization with regulation of gene expression

Nuclear envelope dynamics in connection to chromatin remodeling

Nuclear dynamics: Formation of bodies and trafficking in plant nuclei

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