Ginsenoside-Rg3 inhibits the proliferation and invasion of hepatoma carcinoma cells via regulating long non-coding RNA HOX antisense intergenic

Abstract

Ginsenoside Rg3, a natural compound, has been reported to function as an anticancer agent for hepatoma carcinoma, while the mechanisms underlying the anticancer effects are not clear. Therefore, the objective of our study was to explore the impact of RG3 on cell migration and invasion by regulating the lncRNA HOX antisense intergenic (HOTAIR) expression involving PI3K/ AKT signaling pathway. qRT-PCR was utilized to measure the mRNA expression of HOTAIR. Furthermore, HOTAIR overexpression plasmids were transfected to SMMC-7721 and SK-Hep-1 cells. Additionally, MTT assay was used to evaluate the proliferation of transfected cells. The scratch and transwell assays were used to determine the migration and invasion ability of the cell. The protein levels were determined with Western blot. lncRNA HOTAIR was overexpressed in SMMC-7721 and SK-Hep-1 cells. Ginsenoside-Rg3 reduced the level of lncRNA HOTAIR. Overexpressed lncRNA HOTAIR offset ginsenoside-Rg3 inhibited proliferation, migration and invasion of HCC cells. Furthermore, ginsenoside-Rg3 decreased the expression of p-AKT, p-PI3K, matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9 (MMP9), which was reversed after the treatment of HOTAIR. LncRNA HOTAIR was overexpressed in SMMC-7721 cells. Ginsenoside-Rg3 could reduce the expression of lncRNA HOTAIR, resulting in the inhibited cell proliferation, migration and invasion. Furthermore, ginsenoside-Rg3 inhibited cell proliferation and invasion ability through the PI3k/AKT pathway. Thus, ginsenoside-Rg3 might be a potential and effective treatment for HCC.

Vacuolar protein sorting-associated protein 72 homolog (VPS72) binding to lysine acetyltransferase 5 (KAT5) promotes the proliferation, invasion and migration of hepatocellular carcinoma through regulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway

“…In addition, KAT5 knockdown specifically inhibits the proliferation of gallbladder carcinoma (GBC)-SD cells by casp9-mediated apoptosis [ 28 ]. Some researches claimed that the activation of PI3K/AKT signaling pathway has been implicated in the pathogenesis of hepatocellular carcinoma [ 29 , 30 ]. A case here is that maternally expressed gene 3 promotes hepatocellular carcinoma by activating PI3K/AKT signaling pathway through regulating AP1G1 [ 31 ].…”

Long non-coding RNA SNHG5 regulates ulcerative colitis via microRNA-375 / Janus kinase-2 axis

“…qRT-PCR was used to evaluate mRNA and miRNA levels [ 30 ]. Total RNA of YAMC cells extracted by TRIzol® reagent (Thermo Fisher Scientific, Calif, USA).…”

Targeting lncRNAs of colorectal cancers with natural products

“…Especially CCAT1 suppression by ginsenoside-Rg3 reduced the signaling of the PI3K/Akt pathway, followed by suppression of CRC development ( Li and Qi, 2019 ). In addition, ginsenoside Rg3 suppressed cancer development by suppressing HOTAIR in hepatoma ( Pu et al, 2021 ).…”