Ginsenoside Rg1 Alleviates Podocyte EMT Passage by Regulating AKT/GSK3 <i>β</i>/<i>β</i>-Catenin Pathway by Restoring Autophagic Activity

Shi, Yimin; Gao, Yanbin; Wang, Tao; Wang, Xiaolei; He, Jiaxin; Xu, Jiayi; Wu, Bingjie; Li, Yimeng

doi:10.1155/2020/1903627

Cited by 20 publications

(16 citation statements)

References 44 publications

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“…After 4 weeks of STZ injection, diabetic rats were randomly divided into 3 group (n=8): control group, DN group and Rg1 group. The control group was given distilled water, and the Rg1 group was given 50 mg/kg Rg1 (32), intraperitoneal injection once a day, for 8 weeks. The rats were sacrificed, blood and kidney tissues were collected for further analysis.…”

Section: Animal Models and Sample Collectionmentioning

confidence: 99%

Ginsenoside Rg1 attenuates the inflammation and oxidative stress induced by diabetic nephropathy through regulating the PI3K/AKT/FOXO3 pathway

Liu¹,

Chen²,

Ma³

et al. 2021

Ann Transl Med

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Background: Diabetic nephropathy (DN) is one of the most common microvascular complications in diabetes mellitus. Ginsenoside Rg1 (Rg1) is an important active ingredient extracted from Panax ginseng. This study aimed to investigate the role and molecular mechanism of Rg1 in DN model. Methods: The mesangial cell line HBZY-1 was induced by high glucose (HG; 30 mM D-glucose). HGinduced HBZY-1 cells were treated with Rg1 (2.5, 5, 10 μmol/L). Cell viability was measured by the MTT assay. Apoptosis was detected by Hoechst staining and flow cytometry. Related proteins were measured by western blot. Reactive oxygen species (ROS) production was measured by dichlorodihydrofluorescein diacetate (DCFH-DA). Inflammatory factors and molecules associated with oxidative stress were detected by enzyme-linked immunoassay (ELISA). DN rats model were treated with 50 mg/kg/d Rg1 for 8 weeks, the histopathological changes and the expression of relevant markers were analyzed. Results: We found that Rg1 treatment markedly elevated the survival rates of HG-induced HBZY-1 cells and reduced apoptosis induced by HG. Rg1 treatment attenuated the HG-induced inflammatory response by decreasing the high levels of TNF-α, IL-1β, and IL-6. Furthermore, Rg1 treatment alleviated HGinduced oxidative stress by decreasing ROS generation, malondialdehyde (MDA), and lactate dehydrogenase (LDH) accumulation and increasing the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). After Rg1 (50 mg/kg/d) treatment, the severe glomerular thylakoid hyperplasia, glomerular atrophy, tubule dermal cell exfoliation, basement membrane exposure, interstitial edema and inflammatory cell cytoplasmic infiltration were alleviated in DN rats. DN rat model treated with Rg1 (50 mg/kg/d) showed good anti-inflammatory and antioxidant activities. Rg1 treatment also increased the levels of the phosphorylation of PI3K (p-PI3K) and AKT (p-AKT) and promoted the transfer of FOXO3 from the nucleus to the cytoplasm in vitro and in vivo. Conclusions: Rg1 exhibited protective effects on DN-induced inflammatory responses and oxidative stress via regulating the PI3K/AKT/FOXO3 pathway in in vitro and in vivo. The results suggest that Rg1 may be a potential therapeutic agent for DN treatment.

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Section: Animal Models and Sample Collectionmentioning

confidence: 99%

Ginsenoside Rg1 attenuates the inflammation and oxidative stress induced by diabetic nephropathy through regulating the PI3K/AKT/FOXO3 pathway

Liu¹,

Chen²,

Ma³

et al. 2021

Ann Transl Med

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“…Induces autophagy via inhibiting AKT/mTOR [218,240,241] Perilla frutescens var. acuta Perilaldehyde Induces autophagy via activating AMPK [222,228] Rehmannia glutinosa var.…”

Section: Name Active Components Role In Autophagy Referencesmentioning

confidence: 99%

Autophagy as a Therapeutic Target of Natural Products Enhancing Embryo Implantation

Park

Cho

et al. 2021

Pharmaceuticals

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Infertility is an emerging health issue worldwide, and female infertility is intimately associated with embryo implantation failure. Embryo implantation is an essential process during the initiation of prenatal development. Recent studies have strongly suggested that autophagy in the endometrium is the most important factor for successful embryo implantation. In addition, several studies have reported the effects of various natural products on infertility improvement via the regulation of embryo implantation, embryo quality, and endometrial receptivity. However, it is unclear whether natural products can improve embryo implantation ability by regulating endometrial autophagy. Therefore, we performed a literature review of studies on endometrial autophagy, embryo implantation, natural products, and female infertility. Based on the information from these studies, this review suggests a new treatment strategy for female infertility by proposing natural products that have been proven to be safe and effective as endometrial autophagy regulators; additionally, we provide a comprehensive understanding of the relationship between the regulation of endometrial autophagy by natural products and female infertility, with an emphasis on embryo implantation.

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“…Intriguing new data suggest that Panax ginseng C.A.Mey. could minimize renal injury by inhibiting oxidative stress, inflammatory responses, epithelial-mesenchymal transition, and fibrosis ( Liu et al, 2020 ; Shi et al, 2020 ; Xie et al, 2020 ; Zhu et al, 2020 ; Li et al, 2021 ). This finding is pertinent to the pathologic phenotype of KD, which is characterized by features of destruction of the glomerular filtration barrier involving podocyte, basement membrane and endotheliocyte ( Thomas et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Panax Ginseng C.A.Mey. as Medicine: The Potential Use of Panax Ginseng C.A.Mey. as a Remedy for Kidney Protection from a Pharmacological Perspective

Jin

Zhang

et al. 2021

Front. Pharmacol.

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Panax ginseng C.A.Mey. has been widely consumed as food/diet supplements from natural sources, and its therapeutic properties have also aroused widespread concern. Therapeutic properties of Panax ginseng C.A.Mey. such as anti-inflammatory, ameliorating chronic inflammation, enhancing the immunity, resisting the oxidation again, and regulating the glucose and lipid metabolism have been widely reported. Recent years, lots of interesting studies have reported the potential use of Panax ginseng C.A.Mey. in the management of DKD. DKD has become the leading cause of end-stage renal disease worldwide, which increases the risk of premature death and poses a serious financial burden. Although DKD is somehow controllable with different drugs such as Angiotensin-Converting Enzyme Inhibitors (ACEI), Angiotensin Receptor Blockers (ARB) and lowering-glucose agents, modern dietary changes associated with DKD have facilitated research to assess the preventive and therapeutic merits of diet supplements from natural sources as medicine including Panax ginseng C.A.Mey. Findings from many scientific evidences have suggested that Panax ginseng C.A.Mey. can relieve the pathological status in cellular and animal models of DKD. Moreover, a few studies showed that alleviation of clinical phenotype such as reducing albuminuria, serum creatinine and renal anemia in DKD patients after application or consumption of Panax ginseng C.A.Mey.. Therefore, this review aims to discuss the effectiveness of Panax ginseng C.A.Mey. as medicine for targeting pathological phenotypes in DKD from a pharmacological perspective. This review will provide new insights into the potential understanding use of Panax ginseng C.A.Mey. in the management of DKD in clinical settings.

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Ginsenoside Rg1 Alleviates Podocyte EMT Passage by Regulating AKT/GSK3 β/β-Catenin Pathway by Restoring Autophagic Activity

Cited by 20 publications

References 44 publications

Ginsenoside Rg1 attenuates the inflammation and oxidative stress induced by diabetic nephropathy through regulating the PI3K/AKT/FOXO3 pathway

Ginsenoside Rg1 attenuates the inflammation and oxidative stress induced by diabetic nephropathy through regulating the PI3K/AKT/FOXO3 pathway

Autophagy as a Therapeutic Target of Natural Products Enhancing Embryo Implantation

Panax Ginseng C.A.Mey. as Medicine: The Potential Use of Panax Ginseng C.A.Mey. as a Remedy for Kidney Protection from a Pharmacological Perspective

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