Ginkgolide B Mediated Alleviation of Inflammatory Cascades and Altered Lipid Metabolism in HUVECs via Targeting PCSK-9 Expression and Functionality

Wang, Gang; Liu, Zhenbin; Li, Menghu; Yu, Li; Alvi, Sahir Sultan; Ansari, Irfan Ahmad; Khan, M. Salman

doi:10.1155/2019/7284767

Cited by 23 publications

(28 citation statements)

References 52 publications

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“…GB exerts anti-inflammatory and antioxidant pharmacological effects ( 28 ). In HUVECs, GB mediates the inhibition of inflammatory cascades and alters lipid metabolism through targeting the expression and function of proprotein convertase subtilisin/kexin type 9( 11 ). In addition, GB regulates myocardial inflammation induced by ischemia-reperfusion injury via the A20/NF-κB signaling pathway ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, GB is widely used in the treatment of neurological diseases, including cerebral ischemia ( 10 ). Emerging evidence has suggested that GB exerts anti-inflammatory and neuroprotective effects ( 11 , 12 ). GB alleviates hypoxia-induced hippocampal neuron injury in mice through inhibiting oxidative stress and cell apoptosis ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

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Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS‑induced BV2 cells in vitro and in vivo

2021

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Ginkgolide B (GB) is widely used in the treatment of neurological diseases and exerts anti-inflammatory and neuroprotective effects. Microglia serve an important role in central nervous inflammation. The present study investigated the effect of GB on central nervous inflammation in vivo and in vitro . BV2 cells were activated with lipopolysaccharide (LPS) to establish a cellular model of neuroinflammation. Cell viability was determined using the Cell Counting Kit-8 assay and the secreted levels of TNF-α, IL-1 and IL-6 were measured using ELISAs. The levels of nitric oxide (NO) was assessed using Griess assays. In addition, the mRNA and protein expression levels of inducible NO synthase and cyclooxygenase-2 (COX-2) were detected using reverse transcription-quantitative PCR and western blot analyses, respectively. Transwell assays were carried out to evaluate the cell migratory ability. For the in vivo studies, an LPS-induced neuroinflammation model was established in C57 mice. Western blot analysis and immunohistochemistry were performed to detect the expression of the microglial marker allograft inflammatory factor 1 in the hippocampal dentate gyrus and striatum. The expression levels of TNF-α, IL-1 and IL-6 in the hippocampal dentate gyrus and striatum were assessed using western blot analysis. The results revealed that GB reduced the inflammatory response and migration of LPS-induced BV2 cells. Furthermore, GB attenuated the activation of BV2 cells of the hippocampal dentate gyrus and striatum in the LPS-induced mice with neuroinflammation. Taken together, the findings of the present study demonstrated that GB alleviated the inflammatory response and attenuated the activation of LPS-induced BV2 cells in vitro and in vivo .

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS‑induced BV2 cells in vitro and in vivo

2021

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“…Briefly, a portion of the kidney was homogenized in 0.25 M sucrose and extracted [32]. Soluble proteins in the kidney homogenate were determined by the Bradford method [33], and the concentration of the proteins was adjusted to 1 mg/mL; the level of fluorescent AGEs was assessed using an Agilent (US) spectrofluorometer with a λEx of 370 nm and a λEm range of 380-550 nm.…”

Section: Estimation Of Renal Fluorescent Agesmentioning

confidence: 99%

“…cDNA synthesis The cDNA was produced using the Applied Biosystems™ cDNA Reverse Transcription Kit (Thermo Fisher Sci., India). The reaction mixture (20 μL) containing 2000 ng of RNA and Master Mix was subjected to thermocycling with the following program: 25°C for 10 min, 37°C for 120 min, 85°C for 5 min [19,33].…”

Section: Assay Of Hmg-coa Reductase (Hmg-r) Activitymentioning

confidence: 99%

Amelioration of neuropilin-1 and RAGE/matrix metalloproteinase-2 pathway-induced renal injury in diabetic rats by rosuvastatin

Nabi

Alvi

Alouffi

et al. 2021

Arch biol sci (Beogr)

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Advanced glycation end-products (AGEs) induce the production of reactive oxygen species (ROS) and extra cellular matrix (ECM) degradation via suppression of neuropilin-1 (NRP-1) and interaction with AGE-receptors (RAGE). This study aimed to reveal whether modulation of NRP-1 by rosuvastatin (RT) prevents AGE-induced renal injury via targeting RAGE/matrix metalloproteinase-2 (MMP-2) signaling in diabetic rats. Treatment with RT ameliorated the altered level of markers of glycemic control, renal injury, cholesterol, triglyceride (TG) and hepatic HMG-CoA reductase activity; the level of circulatory carboxymethyl-lysine (CML) and the accumulation of fluorogenic-AGEs in renal tissue was reduced; the expression of renal NRP-1, a checkpoint target, was stimulated; the transcription of RAGE, NF?B-2, TGF-?1 and MMP-2 was suppressed; the circulatory carbonyl content (CC) and paraoxonase-1 (PON-1) activity was ameliorated, and renal histopathological features were attenuated as evidenced by improved glomerular appearance, Bowman?s space and abundant podocytes in kidneys. In conclusion, RT exhibited the potential to counteract diabetes and AGE-induced renal pathologies via stimulation of NRP-1, suppression of RAGE, and of genes responsible for ECM disintegration (MMP-2) and the inflammatory response (NF?B-2).

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“…↓oxidative stress ↓adhesion molecules expression and adhesion to monocytes Feng et al (2018); Li et al (2009);Ma et al (2013);Wang et al (2019) …”

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confidence: 99%

Chinese Herbal Medicines and Active Metabolites: Potential Antioxidant Treatments for Atherosclerosis

et al. 2021

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Atherosclerosis is a complex chronic disease that occurs in the arterial wall. Oxidative stress plays a crucial role in the occurrence and progression of atherosclerotic plaques. The dominance of oxidative stress over antioxidative capacity generates excess reactive oxygen species, leading to dysfunctions of the endothelium and accelerating atherosclerotic plaque progression. Studies showed that Chinese herbal medicines and traditional Chinese medicine (TCM) might regulate oxidative stress; they have already been used to treat diseases related to atherosclerosis, including stroke and myocardial infarction. This review will summarize the mechanisms of oxidative stress in atherosclerosis and discuss studies of Chinese herbal medicines and TCM preparations treating atherosclerosis, aiming to increase understanding of TCM and stimulate research for new drugs to treat diseases associated with oxidative stress.

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Ginkgolide B Mediated Alleviation of Inflammatory Cascades and Altered Lipid Metabolism in HUVECs via Targeting PCSK-9 Expression and Functionality

Cited by 23 publications

References 52 publications

Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS‑induced BV2 cells in vitro and in vivo

Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS‑induced BV2 cells in vitro and in vivo

Amelioration of neuropilin-1 and RAGE/matrix metalloproteinase-2 pathway-induced renal injury in diabetic rats by rosuvastatin

Chinese Herbal Medicines and Active Metabolites: Potential Antioxidant Treatments for Atherosclerosis

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