Gingival crevice microbiota from Chinese patients with gingivitis or necrotizing ulcerative gingivitis

Abstract

The objective of this study was to quantitatively compare the bacterial population structure in plaque from the gingival margin of two groups of 21 Chinese patients with gingivitis or necrotizing ulcerative gingivitis (NUG). Subjects were recruited in four dental clinics in Eastern China. Samples were quantitatively assessed by immunofluorescence and fluorescent in situ hybridization for taxa known to be associated with periodontal diseases. The analyses showed that the fusiform taxa (Fusobacterium nucleatum/Fusobacterium periodonticum, Leptotrichia buccalis, Tannerella forsythensis, and Capnocytophaga sp.), Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens, Selenomonas sputigena, and treponemes were present in both groups with high prevalence. Porphyromonas gingivalis and Actinomyces gerencseriae were much more prevalent in the NUG group. Quantitatively, most taxa, including P. gingivalis, F. nucleatum and the treponemes, accounted, on average, for < 3% of the total bacterial cell number. Only P. intermedia/P. nigrescens, P. gingivalis, S. sputigena, A. gerencseriae, and the sum of all monitored suspected periodontal pathogens were significantly increased in the NUG group. The present study demonstrates for both groups a highly diverse plaque composition and suggests that, etiologically, the overall concentration and the concerted effects of the entire group of opportunistic pathogens thriving in NUG-associated plaque are of prime importance.

Relationship of the mucosal microbiota to gastrointestinal inflammation and small cell intestinal lymphoma in cats

“…(Erec482),29 Bacteroides‐Prevotella group (Bacto1080),30 Fusobacterium spp. (Fuso0664),31 Enterobacteriaceae (Ebac1790),32 Helicobacter spp. (Hel717),33 and Faecalibacterium spp.…”

Actinomyces and Related Organisms in Human Infections

“…Indeed, A. naeslundii has been found in cases of dental plaque-induced gingivitis, although A. naeslundii and A. gerencseriae were also reported to be associated with periodontal health (191). Somewhat surprising was the detection of A. gerencseriae as one of the dominant species of the microbiota at the gingival margin in relation to a severe form of gingival disease, necrotizing ulcerative gingivitis (192). Typically, Gram-negative anaerobes, such as fusobacteria, Prevotella intermedia, and spirochetes, are considered the etiologic organisms of this acute and painful disease, which destroys soft tissues around affected teeth, especially interdental gingival papillae.…”

Fusobacterium nucleatum Infection of Colonic Cells Stimulates MUC2 Mucin and Tumor Necrosis Factor Alpha

“…nucleatum is a known proinflammatory pathogen (20,29,43), and it was of interest to determine if the F. nucleatum strains also stimulated the proinflammatory cytokines TNF-␣ and IL-1␤. As shown in Fig.…”