Gingival and dermal fibroblasts: Their similarities and differences revealed from gene expression

Ebisawa, Katsumi; Kato, Ryuji; Okada, Mai; Sugimura, Tomoko; Latif, Mazlyzam Abdul; Hori, Yusuke S.; Narita, Yuji; Ueda, Minoru; Honda, Hiroyuki; Kagami, Hideaki

doi:10.1016/j.jbiosc.2010.11.014

Cited by 33 publications

(19 citation statements)

References 11 publications

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“…In addition, experiments with cultured cells have suggested that oral mucosal fibroblasts have also some other properties that are distinct from adult skin fibroblasts, but similar to fetal skin cells [5], [12]. Fibroblasts from skin and oral mucosa have also distinct gene expression profiles that correlate with their different embryonic patterning-related HOX gene expression [13]–[16]. This may depend on the different embryonic origin of gingival and skin connective tissue cells, the former being mostly from the neural crest and the latter from the mesoderm [11], [17].…”

Section: Introductionmentioning

confidence: 99%

Human Gingival Fibroblasts Display a Non-Fibrotic Phenotype Distinct from Skin Fibroblasts in Three-Dimensional Cultures

et al. 2014

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Scar formation following skin injury can be a major psychosocial and physiological problem. However, the mechanisms of scar formation are still not completely understood. Previous studies have shown that wound healing in oral mucosa is faster, associates with a reduced inflammatory response and results to significantly reduced scar formation compared with skin wounds. In the present study, we hypothesized that oral mucosal fibroblasts from human gingiva are inherently distinct from fibroblasts from breast and abdominal skin, two areas prone to excessive scar formation, which may contribute to the preferential wound healing outcome in gingiva. To this end, we compared the phenotype of human gingival and skin fibroblasts cultured in in vivo-like three-dimensional (3D) cultures that mimic the cells' natural extracellular matrix (ECM) niche. To establish 3D cultures, five parallel fibroblast lines from human gingiva (GFBLs) and breast skin (SFBLs) were seeded in high density, and cultured for up to 21 days in serum and ascorbic acid containing medium to induce expression of wound-healing transcriptome and ECM deposition. Cell proliferation, morphology, phenotype and expression of wound healing and scar related genes were analyzed by real-time RT-PCR, Western blotting and immunocytochemical methods. The expression of a set of genes was also studied in three parallel lines of human abdominal SFBLs. Findings showed that GFBLs displayed morphologically distinct organization of the 3D cultures and proliferated faster than SFBLs. GFBLs expressed elevated levels of molecules involved in regulation of inflammation and ECM remodeling (MMPs) while SFBLs showed significantly higher expression of TGF-β signaling, ECM and myofibroblast and cell contractility-related genes. Thus, GFBLs display an inherent phenotype conducive for fast resolution of inflammation and ECM remodeling, characteristic for scar-free wound healing, while SFBLs have a profibrotic, scar-prone phenotype.

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Section: Introductionmentioning

confidence: 99%

Human Gingival Fibroblasts Display a Non-Fibrotic Phenotype Distinct from Skin Fibroblasts in Three-Dimensional Cultures

et al. 2014

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“…In addition, gene expression profiles of fibroblasts isolated from human skin and gingiva and other comparable studies further indicate that gingival fibroblasts are distinct from the skin fibroblasts from various body sites (12,82). Moreover, comparison of 5284 genes in fibroblasts from human gingiva and facial skin showed significantly different expression of 278 genes (5%) between these two cell types, and gene ontology hierarchy analysis showed that the major differences were associated with ECM, oxidoreductases, cytokines and growth factor activityerelated genes (83). Thus, different embryonic origin and patterning appears to result in distinct phenotypic characteristics between gingival and skin fibroblasts (Table II).…”

Section: Developmental Origin and Gene Expressionmentioning

confidence: 59%

Distinct phenotype and therapeutic potential of gingival fibroblasts

2014

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“…To validate the findings based on the FANTOM5 dataset, 3 microarray datasets which evaluated various fibroblasts including GFs were analyzed focusing on GFs 12 15 16 . Differential gene expression was determined following the criteria, FDR < 0.05 and log 2 fold change >1 or <−1.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of periodontitis-associated fibroblasts by CAGE sequencing identified DLX5 and RUNX2 long variant as novel regulators involved in periodontitis

Horie

Yamaguchi

Saito

et al. 2016

Sci Rep

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Periodontitis is affecting over half of the adult population, and represents a major public health problem. Previously, we isolated a subset of gingival fibroblasts (GFs) from periodontitis patients, designated as periodontitis-associated fibroblasts (PAFs), which were highly capable of collagen degradation. To elucidate their molecular profiles, GFs isolated form healthy and periodontitis-affected gingival tissues were analyzed by CAGE-seq and integrated with the FANTOM5 atlas. GFs from healthy gingival tissues displayed distinctive patterns of CAGE profiles as compared to fibroblasts from other organ sites and characterized by specific expression of developmentally important transcription factors such as BARX1, PAX9, LHX8, and DLX5. In addition, a novel long non-coding RNA associated with LHX8 was described. Furthermore, we identified DLX5 regulating expression of the long variant of RUNX2 transcript, which was specifically active in GFs but not in their periodontitis-affected counterparts. Knockdown of these factors in GFs resulted in altered expression of extracellular matrix (ECM) components. These results indicate activation of DLX5 and RUNX2 via its distal promoter represents a unique feature of GFs, and is important for ECM regulation. Down-regulation of these transcription factors in PAFs could be associated with their property to degrade collagen, which may impact on the process of periodontitis.

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Gingival and dermal fibroblasts: Their similarities and differences revealed from gene expression

Cited by 33 publications

References 11 publications

Human Gingival Fibroblasts Display a Non-Fibrotic Phenotype Distinct from Skin Fibroblasts in Three-Dimensional Cultures

Human Gingival Fibroblasts Display a Non-Fibrotic Phenotype Distinct from Skin Fibroblasts in Three-Dimensional Cultures

Distinct phenotype and therapeutic potential of gingival fibroblasts

Transcriptome analysis of periodontitis-associated fibroblasts by CAGE sequencing identified DLX5 and RUNX2 long variant as novel regulators involved in periodontitis

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