Distinct phenotype and therapeutic potential of gingival fibroblasts

Häkkinen, Lari; Larjava, Hannu; Fournier, Benjamin

doi:10.1016/j.jcyt.2014.04.004

Cited by 65 publications

(66 citation statements)

References 156 publications

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“…Alkaline Phosphatase (ALP) specific activity was used in this study (with Alizarin red assay), to verify the osteogenic differentiation of HGFs; this enzyme, in fact, was one of the first markers identified to assess the osteogenic phenotype . Some papers have already demonstrated the ability of HGFs to differentiate as osteoblasts whereas other authors showed opposed results . ‐ Even if this topic is under debate, a recent review is also in favor of the ability of HGFs to assume the osteoblast phenotype, thus for the purpose of the present study, this system is suitable to investigate the effect of DXM release from NPs.…”

Section: Resultsmentioning

confidence: 99%

Dexamethasone-loaded biopolymeric nanoparticles promote gingival fibroblasts differentiation

Chronopoulou

Amalfitano

Palocci

et al. 2015

Biotechnol Progress

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Polymer-based nanoparticles (NPs) can be efficiently used for the delivery of bioactive molecules for both in vitro and in vivo applications affording high drug loading and controlled release profiles. Within this framework polylactic-co-glycolic acid (PLGA) NPs with a diameter of 290 ± 41 nm have been fabricated and loaded with dexamethasone (DXM) using a patented procedure. The aim of the project was to setup a controlled delivery system to promote the in vitro differentiation of Human Gingival Fibroblasts (HGFs). First the uptake of fluorescent PLGA NPs by HGFs cells was investigated; then experiments were also addressed to analyze the specific cell response to DXM, in order to evaluate its functional efficiency in comparison with its conventional addition to the culture medium. The results showed that cells treated with DXM-loaded NPs acquired the osteoblast phenotype faster in comparison to those treated with the free drug. The slow and sustained release of DXM from PLGA NPs produced a constant and uniform concentration of drug inside cells with long-term and enhanced biochemical effects.

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Section: Resultsmentioning

confidence: 99%

Dexamethasone-loaded biopolymeric nanoparticles promote gingival fibroblasts differentiation

Chronopoulou

Amalfitano

Palocci

et al. 2015

Biotechnol Progress

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“…Cells do not have a cuboid shape, rather have a flat surface were considered as surface lining cells which are not active osteoblast producing osteoid and were not counted. Regarding the inflammatory cells, fibroblast cells and endothelial cells could easily be differentiated from inflammatory cells which were macrophage, neutrophil, eosinophil, T lymphocytes, and plasma cells . Fibroblasts have a thin and long spindle‐like shape, while other cells have a more rounded shape .…”

Section: Methodsmentioning

confidence: 99%

“…Regarding the inflammatory cells, fibroblast cells and endothelial cells could easily be differentiated from inflammatory cells which were macrophage, neutrophil, eosinophil, T lymphocytes, and plasma cells. [28][29][30][31][32][33] Fibroblasts have a thin and long spindle-like shape, while other cells have a more rounded shape. 28,29,[31][32][33][34] The cells which were not easily differentiated were not counted.…”

Section: Histologic Evaluationmentioning

confidence: 99%

Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Taşkan

Yüce

Karataş

et al. 2019

J of Periodontal Research

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The Objective The present study aimed to evaluate the effects of oleuropein on ligature‐induced alveolar bone loss. In this respect, osteoblastic activity, osteoclastic activity, inflammatory markers, and apoptosis were evaluated. Background Oleuropein is a flavonoid, which has potent anti‐inflammatory and bone‐protective effects. Methods Thirty‐two Wistar rats were divided into four experimental groups as following: control (C, n = 8) group; periodontitis (P, n = 8) group; periodontitis and low‐dose oleuropein group (12 mg/kg/day oleuropein, LDO group, n = 8); and periodontitis and high‐dose oleuropein group (24 mg/kg/day oleuropein, HDO group, n = 8). Periodontitis was induced via ligatures. Study period was 14 days, and animals were sacrificed at end of this period. Mandibles were examined via a stereomicroscope and underwent histological procedures. Osteoblast, tartrate‐resistant acid phosphatase (TRAP)‐positive osteoclast, and inflammatory cell counts were determined in hematoxylin‐eosin stained sections. Inducible nitric oxide synthase (iNOS), bone morphogenetic protein‐4, the cluster of differentiation (CD)‐68, cysteine‐aspartic proteases‐3 (Caspase 3), and B‐cell lymphoma‐2 (Bcl‐2) expressions were evaluated via immunohistochemistry. Results Periodontitis group had highest alveolar bone loss, and these levels significantly decreased in LDO and HDO groups. Both 12 and 24 mg/kg oleuropein groups significantly increased osteoblast cell counts and decreased TRAP‐positive osteoclast and inflammatory cell counts. BMP‐4 and bcl‐2 expressions were elevated in oleuropein groups while caspase‐3 expressions decreased. iNOS and CD68 were higher in periodontitis group compared to control group, but there was no significant difference between other groups. Conclusion Oleuropein successfully decreased alveolar bone loss as a result of decreased osteoclastic activity, inflammation, and apoptosis and increased osteoblastic activity.

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“…Fibroblasts are ubiquitous and heterogeneous mesenchymal cells present in many connective tissues (Moule et al 1995, H€ akkinen et al 2014, Sriram et al 2015. Present in the subodontoblast layer and in the core of the dental pulp, fibroblasts allow the extracellular matrix turnover (Mj€ or et al 2001, Goldberg & Smith 2004.…”

Section: Introductionmentioning

confidence: 99%

Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts

et al. 2017

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Le Clerc J, Tricot-Doleux S, Pellen-Mussi P, P erard M, Jeanne S, P erez F. Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts. International Endodontic Journal, 51, e94-e106, 2018.Aim To investigate in human dental pulp fibroblasts (HDPF) the expression of factors involved in dental pulp physiopathological processes and in an experimental model of cell activation called nemosis, and to compare the behaviour of pulp cell activation with sound lung fibroblast MRC5, employed as a reference model for nemosis. Methodology Nemotic response was induced in three-dimensional cultures of HDPF and lung fibroblasts. The expressions of molecules involved in physiological (alkaline phosphatase, type I collagen) and in inflammatory processes (IL-6, CXCL8, CCL20, COX-2) were studied using real-time PCR. Concentrations of IL-6 and CXCL8 were analysed during 4 days with ELISA. Nonparametric tests were used to determine statistical differences between groups. Results A significant decrease (P < 0.001) in type I collagen and alkaline phosphatase was observed in MRC5 and HDPF nemotic responses. Although the amounts of mRNA differed between these cell types, there was an increase in CCL20, CXCL8 and COX-2 expression (P < 0.001). Unlike HDPF, MRC5 spheroids displayed significant amounts of IL-6 concentrations and mRNA expression. Notably, increased concentrations of CXCL8 were recorded in all threedimensional cultures compared with monolayers as a function of time (P < 0.05). Conclusion Although the nemotic responses observed were not identical in the pulpal and lung fibroblasts, similarities occurred in the expression of chemokines and cyclooxygenase-2. Nemotic reactions and inflammatory processes in pulp diseases share similarities in terms of the expression of factors. Thus, this in vitro model could constitute a powerful tool to study intercellular relations within the dental pulp and to develop new local treatments to counteract the inflammatory reaction that occurs during pulpitis.

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Distinct phenotype and therapeutic potential of gingival fibroblasts

Cited by 65 publications

References 156 publications

Dexamethasone-loaded biopolymeric nanoparticles promote gingival fibroblasts differentiation

Dexamethasone-loaded biopolymeric nanoparticles promote gingival fibroblasts differentiation

Evaluation of the effect of oleuropein on alveolar bone loss, inflammation, and apoptosis in experimental periodontitis

Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts

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