Ginger extract has anti-leukemia and anti-drug resistant effects on malignant cells

Babasheikhali, Somayeh Rahimi; Rahgozar, Soheila; Mohammadi, Mahboubeh

doi:10.1007/s00432-019-02949-5

Cited by 22 publications

(25 citation statements)

References 31 publications

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“…Recently, a study showed that treatment with 2 mg/ml of ginger with 31 mg/ml of gelam honey inhibited the growth of most HT29 cells and induced apoptosis in a dose-dependent manner, with the combined treatment yielding the highest apoptosis rate. This was caused by the downregulation of gene expression of Akt, mTOR, and Raptor, while cytochrome C and caspase 3 genes where shown to be upregulated 26 Recently, ginger root extract also showed anti-leukemia and anti-drug resistant effects 27 . In the present study, we show that ginger extracted by Soxhlet has stronger cytotoxic activity on HepG2 cells than extracts from Maceration and Sonication.…”

Section: Discussionmentioning

confidence: 99%

Ethanol extract of Ginger Zingiber officinale Roscoe by Soxhlet method induces apoptosis in human hepatocellular carcinoma cell line

Nguyen

et al. 2019

Biomed. Res. Ther.

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Introduction: Ginger has had a long history of use in healthcare in Asian countries, including Vietnam. Internationally, there have been a few studies done to assess the anticancer activity and potency of ginger. In this study, we investigated the activity (cytotoxicity) of various ginger root extracts that were produced by different preparation methods on human hepatocellular carcinoma cell line (HepG2 cell line). Methods: Ginger (Zingiber officinale Roscoe) root extract were extracted from the fresh ginger roots by three methods, which included maceration (G1), Soxhlet (G3), and ultrasonic (G5) methods. The extracts were then evaluated for cytotoxic activity on HepG2 hepatocellular carcinoma cell line based on IC 50 calculation. The most potent extract was then evaluated for its cytotoxicity by Propidium iodide (PI) staining method and microscopic examination by fluorescence microscopy for both 2D and 3D culture conditions. After treatment, cells were also stained with Annexin-V to assess the apoptosis-inducing ability of the extract. Results: The results showed that the ginger root extract prepared by the Soxhlet method (G3) had the strongest activity, and showed significant differences compared to extracts from methods G1 and G5, on the hepatocellular carcinoma cell lines cultured in both 2D and 3D conditions. Indeed, the IC 50 value of ginger root extract of the G3 was 83.3 ± 0.9189 µg/ml, compared to 159 ± 7.6 µg/ml of G1, and 284 ± 5.116 µg/ml of G5 in 2D culture latform; the IC 50 value was 228 ± 33.52µg/ml for G3 compared to 341 ± 3.93 µg/ml for G1, and 603.7 ± 56.33 µg/ml for G5 in 3D culture platform. The results of PI staining of cells in 2D and 3D culture conditions showed that ginger root extract killed HepG2 cells in a concentration-dependent manner. The nuclei of HepG2 cells in 2D culture showed nuclear fragmentation when treated with ginger root extract; this is one of the apoptosis indicators. Moreover, Annexin-V staining results showed that HepG2 cells underwent apoptosis when treated with ginger root extract. Conclusion: The results of the study show that ginger root extract prepared by the Soxhlet method induced strong cytotoxicity of hepatocellular carcinoma cells cultured in both 2D and 3D conditions, through induction of apoptosis. The evidence suggests that ginger is a potential agent for anticancer therapy. It is necessary to perform further research to isolate compounds from fresh ginger root extract by the Soxhlet method to evaluate the mechanism of anti-tumor activity.

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Section: Discussionmentioning

confidence: 99%

Ethanol extract of Ginger Zingiber officinale Roscoe by Soxhlet method induces apoptosis in human hepatocellular carcinoma cell line

Nguyen

et al. 2019

Biomed. Res. Ther.

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“…www.nature.com/scientificreports/ Effect of ginger extract on CCRF-CEM and dexamethasone resistant CCRF-CEM/MVCD subline. The cytotoxic effect of ginger extract on the in house multidrug resistant CCRF-CEM/MVCD subline was previously defined 14 , and RT-PCR showed overexpression of FASN in this cell line compared with normal MNCs and its parental cell (P = 0.0119 and P = 0.0241, respectively) ( Fig. 4a).…”

Section: Effect Of Ginger Extract On Fasn Expression In Primary All Cmentioning

confidence: 64%

“…Our group previously reported the anti-leukemic effect of ginger extract. On the other hand, It was shown that fresh normal peripheral MNCs were not significantly sensitive to proliferation inhibition induced by 50% inhibition concentration of the ginger extract 14 . Furthermore, it was shown that this effect was not ascribed to the expression levels of ABC transporters.…”

Section: Discussionmentioning

confidence: 94%

Fatty acid synthase, a novel poor prognostic factor for acute lymphoblastic leukemia which can be targeted by ginger extract

Ghaeidamini

Rahgozar

Rahimi

et al. 2020

Sci Rep

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Altered metabolism of fatty acid synthesis is considered a hallmark characteristic of several malignancies, including acute lymphoblastic leukemia (ALL). to evaluate the impact of fatty acid synthase (FASN) on drug resistant ALL, bone marrow samples were collected from 65 pediatric ALLs, including 40 de novo and 25 relapsed patients. 22 non-cancer individuals were chosen as controls. Quantitative RT-PCR showed increased expression levels of FASN in drug resistant patients compared with the therapy responders. Single and combined treatment of malignant cells were analyzed using Annexin-V/PI double staining and MTT assays. Incubation of resistant primary cells with ginger showed simultaneous increased apoptosis rates and reduced FASN expression levels. Furthermore, docking studies demonstrated high affinity bindings between ginger derivatives and FASN thioesterase and ketosynthase domains, compared with their known inhibitors, fenofibrate and morin, respectively. Finally, combined treatment of in-house multidrug resistant TALL subline with ginger and dexamethasone induced drug sensitivity and down regulation of FASN expression, accordingly. To the best of our knowledge, this is the first study that introduces FASN upregulation as a poor prognostic factor for drug resistant childhood ALL. Moreover, it was revealed that fASn inhibition may be applied by ginger phytochemicals and overcome dexamethasone resistance, subsequently. Acute lymphoblastic leukemia (ALL) is the most common type of hematological malignancy in children 1,2. Despite the enormous advances in modern medicine and development of innovative therapeutic strategies, disease relapse remains a leading cause of cancer-related morbidity and mortality in children 3. Metabolic rearrangements are vital to satisfy the different requirements of cancer cells during tumorigenesis 4. Elevated de novo fatty acid biosynthesis is a hallmark adaptation in many cancers that supply signaling molecules and basic elements for lipid biosynthesis 5. While most normal cells supply their fatty acids from dietary sources, cancer cells reactivate de novo fatty acid synthesis 6. Fatty acid synthase (FASN) is a multifunctional protein containing six enzymatic domains that catalyzes the biosynthesis of palmitate 5. Elevated expression of FASN is found to be associated with poor prognosis and higher risk of recurrence in a number of human cancers. Indeed, FASN overexpression has been shown to contribute to multidrug resistance (MDR). Multi-drug resistance is one of the major obstacles to the successful treatment of various types of cancer, particularly childhood ALL 5,7,8. Glucocorticoids (GCs) such as prednisone and dexamethasone (DEX) are indispensable drugs for childhood ALL treatment 9. Early response to glucocorticoids is a positive prognostic indicator and glucocorticoid resistance has been associated with an increased risk of relapse and poor clinical response 10,11. Glucocorticoids regulate FASN expression and subsequently affect lipogenesis 12. Therefore, FASN knock down or...

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“…Allowed costs for leukemia patients averaged almost $157,000 in the year after diagnosis, with costs for AML almost tripling that amount, according to a new report from the Leukemia & Lymphoma Society (LLS) [202] (Table 2), (Figure 4). Combined with MTX showed synergistic effects on CCRF-CEM, Nalm-6 and ALL primary cells [203]. [8]-shogaol, originated from ginger, elevated the level of ROS, ccaspase-3, -9, c-PARP, c-DFF-45, and decreased the level of glutathione, MMP, caspase-8 and Bid [204].…”

Section: Blood and Bone Marrow Cancer (Leukemia)mentioning

confidence: 97%

Alternative Treatments for Cancer Prevention and Cure [Part 1]

Mohiuddin¹

2019

APCT

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Many lay people along with some so called "key opinion leaders" have a common slogan "There's no answer for cancer". Again, mistake delays proper treatment and make situation worse, more often. Compliance is crucial to obtain optimal health outcomes, such as cure or improvement in QoL. Patients may delay treatment or fail to seek care because of high out-of-pocket expenditures. Despite phenomenal development, conventional therapy falls short in cancer management. There are two major hurdles in anticancer drug development: dose-limiting toxic side effects that reduce either drug effectiveness or the QoL of patients and complicated drug development processes that are costly and time consuming. Cancer patients are increasingly seeking out alternative medicine and might be reluctant to disclose its use to their oncology treatment physicians. But there is limited available information on patterns of utilization and efficacy of alternative medicine for patients with cancer. As adjuvant therapy, many traditional medicines shown efficacy against brain, head and neck, skin, breast, liver, pancreas, kidney, bladder, prostate, colon and blood cancers. The literature reviews non-pharmacological interventions used against cancer, published trials, systematic reviews and meta-analyses.

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Ginger extract has anti-leukemia and anti-drug resistant effects on malignant cells

Cited by 22 publications

References 31 publications

Ethanol extract of Ginger Zingiber officinale Roscoe by Soxhlet method induces apoptosis in human hepatocellular carcinoma cell line

Ethanol extract of Ginger Zingiber officinale Roscoe by Soxhlet method induces apoptosis in human hepatocellular carcinoma cell line

Fatty acid synthase, a novel poor prognostic factor for acute lymphoblastic leukemia which can be targeted by ginger extract

Alternative Treatments for Cancer Prevention and Cure [Part 1]

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