Giganteaside D induces ROS-mediated apoptosis in human hepatocellular carcinoma cells through the MAPK pathway

Liu, Junshan; Wei, Xiduan; Wu, Yafeng; Wang, Yanni; Qiu, Yudong; Shi, Jun-Min; Zhou, Huixia; Lu, Zibin; Shao, Meng; Yu, Linzhong; Li, Tong

doi:10.1007/s13402-016-0273-9

Cited by 26 publications

(17 citation statements)

References 27 publications

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“…As expected, SB202190 and SP600125 had no effect on the mRNA expression of the NOX subunits (P>0.05). These results are consistent with those of previous studies, which also indicated that increased ROS production may be abolished by NAc and apocynin (21,22).…”

Section: Effect Of Ethanol On Ros Generation In L-02 Cellssupporting

confidence: 94%

NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells

Yang

Zhong

et al. 2018

Int J Mol Med

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The aim of the present study was to assess the molecular mechanism of ethanol‑induced oxidative stress‑mediated apoptosis in L‑02 liver cells in order to elucidate novel pathways associated with alcoholic liver disease. L‑02 cells were treated with 400 mM ethanol with or without inhibitors. The cell viability was measured by an MTT assay. Cell apoptosis was assessed by flow cytometry and a single‑stranded DNA (ssDNA) assay. Intracellular reactive oxygen species (ROS) production of L‑02 cells was determined using the 2',7'‑dichlorofluorescein‑diacetate dye. The protein expression of c‑Jun N‑terminal kinase (JNK), phosphorylated (p)‑JNK, P38, p‑P38, NADPH oxidase (NOX)1, NOX4, p22phox, B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein were measured by western blot analysis. The mRNA expression of NOX1, NOX4 and p22phox was measured by reverse transcription polymerase chain reaction analysis. The results indicated that after treatment with various concentrations of ethanol for the indicated durations, L‑02 cells were displayed a significant decrease in cell viability in a dose‑and time‑dependent manner. Ethanol‑induced apoptosis and cell death of L‑02 cells was accompanied by the generation of ROS, elevated expression of NOX, as well as phosphorylation of JNK and P‑38. In addition, increased expression of Bcl‑2 was induced by 400 mM ethanol. Furthermore, treatment with NOX inhibitor attenuated the ethanol‑induced a decrease in cell viability, and an increase in apoptosis and Bcl‑2 expression. In conclusion, ethanol induced apoptosis in the L‑02 hepatocyte cell line via generation of ROS and elevated expression of NOX4. This indicated that activation of JNK and p38 in the mitogen‑activated protein kinase pathway promotes apoptosis in L‑02 cells.

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Section: Effect Of Ethanol On Ros Generation In L-02 Cellssupporting

confidence: 94%

NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells

Yang

Zhong

et al. 2018

Int J Mol Med

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“…The very poor prognosis and high recurrence rate of HCC is due to tumor invasiveness, frequent intrahepatic spread, and extrahepatic metastasis. Although numerous studies have demonstrated that a complex gene network is involved in the tumorigenesis and progression of HCC, the detailed regulatory mechanisms of key pathways remain obscure . Therefore, improvements in understanding the molecular processes involved in HCC could reveal new monitoring methods and treatment targets for improving HCC diagnosis and treatment.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the metastasis‐associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma

Wang

et al. 2017

J of Gastro and Hepatol

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“…Excessive alcohol intake can lead to overexpression of ROS, leading to oxidative stress and causing apoptosis [50,51]. SOD and GGT play an important role in oxidative stress, while bcl-2 and bax have a very important role in cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of the Polysaccharide Precursor, D-Isofloridoside, from Laurencia undulata on Alcohol-Induced Hepatotoxicity in HepG2 Cells

et al. 2020

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Alcoholic liver disease (ALD) threatens human health, so it is imperative that we find ways to prevent or treat it. In recent years, the study of polysaccharides has shown that they have different kinds of bioactivities. Among them are many biological effects that have been attributed to polysaccharide precursors. D-Isofloridoside (DIF) is one of the polysaccharide precursors from the marine red alga Laurencia undulata. This study evaluated the effect of DIF on alcohol-induced oxidative stress in human hepatoma cells (HepG2). As a result, DIF attenuated alcohol-induced cytotoxicity, reduced the amount of intracellular reactive oxygen species (ROS), and effectively reduced alcohol-induced DNA damage in HepG2 cells. In addition, a western blot showed that, after DIF treatment, the expression levels of glutathione (GSH), superoxide dismutase (SOD), and B-cell lymphoma-2 (bcl-2) increased, while the expression levels of γ-glutamyl transferase (GGT), BCL2-associated X (bax), cleaved caspase-3, and mitogen-activated protein kinase (p38 and c-Jun N-terminal kinase) signal transduction proteins reduced. This showed that DIF may protect cells by reducing the amount of intracellular ROS and inhibiting intracellular oxidative stress and apoptotic processes. Finally, molecular docking demonstrated that DIF can bind to SOD, GGT, B-cell lymphoma-2, and bax proteins. These results indicated that DIF can protect HepG2 cells from alcohol-induced oxidative stress damage, making it an effective potential ingredient in functional foods.

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Giganteaside D induces ROS-mediated apoptosis in human hepatocellular carcinoma cells through the MAPK pathway

Abstract: From our results we conclude that GD can induce ROS-mediated apoptosis in HCC-derived cells through the MAPK pathway. This observation may open up avenues to explore the future use of GD as a HCC chemotherapeutic agent.

Cited by 26 publications

References 27 publications

NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells

NOX4/ROS mediate ethanol‑induced apoptosis via MAPK signal pathway in L‑02 cells

Overexpression of the metastasis‐associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma

The Protective Effect of the Polysaccharide Precursor, D-Isofloridoside, from Laurencia undulata on Alcohol-Induced Hepatotoxicity in HepG2 Cells

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