The Protective Effect of the Polysaccharide Precursor, D-Isofloridoside, from Laurencia undulata on Alcohol-Induced Hepatotoxicity in HepG2 Cells

Abstract

Alcoholic liver disease (ALD) threatens human health, so it is imperative that we find ways to prevent or treat it. In recent years, the study of polysaccharides has shown that they have different kinds of bioactivities. Among them are many biological effects that have been attributed to polysaccharide precursors. D-Isofloridoside (DIF) is one of the polysaccharide precursors from the marine red alga Laurencia undulata. This study evaluated the effect of DIF on alcohol-induced oxidative stress in human hepatoma cells (HepG2). As a result, DIF attenuated alcohol-induced cytotoxicity, reduced the amount of intracellular reactive oxygen species (ROS), and effectively reduced alcohol-induced DNA damage in HepG2 cells. In addition, a western blot showed that, after DIF treatment, the expression levels of glutathione (GSH), superoxide dismutase (SOD), and B-cell lymphoma-2 (bcl-2) increased, while the expression levels of γ-glutamyl transferase (GGT), BCL2-associated X (bax), cleaved caspase-3, and mitogen-activated protein kinase (p38 and c-Jun N-terminal kinase) signal transduction proteins reduced. This showed that DIF may protect cells by reducing the amount of intracellular ROS and inhibiting intracellular oxidative stress and apoptotic processes. Finally, molecular docking demonstrated that DIF can bind to SOD, GGT, B-cell lymphoma-2, and bax proteins. These results indicated that DIF can protect HepG2 cells from alcohol-induced oxidative stress damage, making it an effective potential ingredient in functional foods.

Mechanism Analysis of Antiangiogenic d-Isofloridoside from Marine Edible Red algae Laurencia undulata in HUVEC and HT1080 cell

“…DIF purified from Laurencia undulata , and demonstrated that it has antioxidant and metal matrix protease inhibitory activity in our previous study. , DIF: 1 H NMR (400 MHz, DMSO- d 6 ) δ H 3.47 (6H, m, H-1′,H-3′, H-6), 3.76 (1H, t, J = 2.6 Hz, H-5), 3.69 (1H, brs, H-2), 3.51 (2H, m, H-3, H-4), 3.41 (1H, m, H-2′), 4.35 (1H, m, HO-2), 4.47 (1H, m, HO-2′), 4.81 (1H, d, J = 2.6 Hz, H-1), 4.56 (1H, m, HO-4), 4.58 (1H, m, HO-6), 4.44 (1H, m, HO-1′), 4.38 (1H, m, HO-3); 13 C NMR (100 MHz, DMSO- d 6 ) δ C 68.9 (C-4), 60.6 (C-1′), 80.6 (C-2′), 71.3 (C-5), 99.4 (C-1), 69.7 (C-3), 61.0 (C-6), 60.8 (C-3′), 68.8 (C-2); LREIMS m / z 91.05 (38.57) [M-C 6 H 11 O 4 ] + , 163.10 (11.34) [M-C 3 H 3 O 3 ] + , 255.20 (0.09) [M + H] + . HT1080 cell and HUVEC were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).…”

“…Floridoside is one of the main products of carbon fixation in the process of photosynthesis, and can be used as a precursor of cell wall polysaccharides . Previous studies have shown that DIF has the effect of inhibiting liver cell damage caused by alcoholic oxidative stress and the activity of MMP-2 and MMP-9. , In addition, substances with good antioxidant activity have a certain potential for antitumor activity. , Therefore, this study used DIF as the experimental material to study the effect of DIF on tumor cell invasion, metastasis and angiogenesis.…”

“…Therefore, eating natural foods and their extracts rich in antioxidants is an effective way to prevent tumors . According to early experiments, d -isofloridoside (DIF) extracted from the marine edible brown algae Laurencia undulata have a significant inhibitory effect on oxidative stress and the activity of matrix metalloproteinases. , However, there is no report on the molecular mechanism of DIF in tumor invasion, metastasis, and angiogenesis.…”

Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells

“…The secondary antibody (dilution ratio of 1: 2,000) was incubated for 2 h and then washed with TBST three times. Protein expression levels were detected by using enhanced chemiluminescence substrates ( Yang et al, 2020 ).…”

The Inhibition Effect of the Seaweed Polyphenol, 7-Phloro-Eckol from Ecklonia Cava on Alcohol-Induced Oxidative Stress in HepG2/CYP2E1 Cells

“…Due to the production of reactive oxygen species (ROS) during alcohol metabolism, the liver is vulnerable to oxidative stress-induced injury [ 5 ]. Oxidative stress induced by free radicals has been reported to play a key role in the degeneration, inflammation, apoptosis, and necrosis of hepatocytes [ 6 ].…”