Gicerin, an Ig‐superfamily cell adhesion molecule, promotes the invasive and metastatic activities of a mouse fibroblast cell line

Tsukamoto, Yasuhiro; Egawa, Michiko; Hiroi, Satoshi; Furuya, Masaru; Tsuchiya, Seiichiro; Sasaki, Fumihiko; Miki, Naomasa; Taira, Eiichi

doi:10.1002/jcp.10348

Cited by 8 publications

(9 citation statements)

References 14 publications

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“…Interestingly, we found gicerin to be overexpressed in some sporadic tumors of animals, including Wilm's tumor, oviductal adenocarcinomas, lymphatic sarcomas, melanoma, and mammary gland adenocarcinomas (9)(10)(11)(12). In addition, we also previously showed gicerin transfection to promote the formation of gicerin aggregates, while increasing both migration and metastasis, as determined by in vitro analyses and cell implantation experiments using animals (11,13).…”

Section: Introductionsupporting

confidence: 52%

“…Gicerin interacts with itself, as well as with a laminin-like molecule, and probably with other unidentified protein (5). These adhesive activities of gicerin promote the in vitro migration of embryonic neural cells and primary oviductal cancer cells (4,13,18,19). In addition, the expression of HEMCAM, which is the same molecule as gicerin, in mouse fibroblasts can down-regulate the maturation of ß-1 integrins and decrease the integrin-dependent cell adhesion of laminin ß-1, fibronectin and vitronectin (20).…”

Section: Discussionmentioning

confidence: 99%

“…Each of the transfected cell lines was grown on the cover slips to semiconfluency and then was fixed in Zamboni's fixative solution. The cover slips were washed twice with PBS and incubated with the polyclonal rabbit antibody against gicerin for 1 h at 37˚C (13). After being washed twice with PBS, the cover slips were incubated with fluorescein isothiocyanate-conjugated goat anti-rabbit immunoglobulins (Dako) for 1 h at 37˚C.…”

Section: Establishmentmentioning

confidence: 99%

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Involvement of gicerin, a cell adhesion molecule, in the portal metastasis of rat colorectal adenocarcinoma cells

Tsukamoto¹

2010

Oncol Rep

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Abstract. Gicerin, an Ig-superfamily cell adhesion molecule, has homophilic adhesion activity, thus leading to the formation of gicerin aggregates. Gicerin is highly expressed in various embryonic tissues, and it contributes to development through its adhesive activities. In contrast, the expression of the protein is limited to the muscular tissues and endothelial cells in the mature animals. In the liver, gicerin is constitutively expressed in sinusoidal endothelial cells. Interestingly, an overexpression of gicerin is found in a variety of tumors and may play a role in tumorigenesis. Previously, up-regulated expression of the gicerin protein was found in some sporadic cases of chicken colorectal adenocarcinomas and their hepatic metastasized lesions. In the present study, gicerin cDNA was introduced into endogenous gicerin negative ACL-15 cells, a rat colon adenocarcinoma cell line. The cells were subsequently evaluated for changes in their metastatic potentials in order to elucidate the possible role of gicerin in the hepatic metastasis of colorectal adenocarcinomas. The stable overexpression of gicerin in the cells enhanced the self-aggregation and migratory activities on the protein compared with the mock-transfectants. In addition, the gicerintransfectants had enhanced metastatic potential to the liver compared with mock-transfected cells after implantation into the ileocolic vein of the cognate rats. These results suggest that gicerin might promote the interaction of tumor cells with a hepatic endothelium, thus leading to the hepatic metastasis of colon adenocarcinomas.

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Section: Introductionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Establishmentmentioning

confidence: 99%

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Involvement of gicerin, a cell adhesion molecule, in the portal metastasis of rat colorectal adenocarcinoma cells

Tsukamoto¹

2010

Oncol Rep

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“…Like most other cell adhesion molecules in the Ig-superfamily, gicerin has cell adhesive activities with its ligands (8)(9)(10)(11)22). We have previously reported that gicerin in regenerating cells might participate in the reconstruction of injured tissues through its cell adhesion properties; namely, cell-cell connections in the remodeling epithelium are promoted by gicerin-gicerin interaction, while cell-ECM binding is also enhanced by gicerin-NOF interaction (7).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, endothelial cells express gicerin continuously even after maturation. Interestingly, gicerin is overexpressed in some sporadic tumors, and it also promotes binding among tumor cells and the adhesion of tumor cells to ECM, thus leading to tumor progression (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Involvement of transforming growth factor-β in the expression of gicerin, a cell adhesion molecule, in the regeneration of hepatocytes

Tsuchiya

Tsukamoto²,

Taira³

et al. 2007

Int J Mol Med

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Abstract. Gicerin, an Ig-superfamily cell adhesion molecule, appears transiently in embryonic tissues including those of the nervous, urogenital, respiratory and digestive systems, and it promotes neurite extension, cell migration and epithelialization through its cell adhesive activities. In addition, gicerin also reappears in regenerating tissue after suffering either a traumatic injury or a viral infection. In the present study, we examined the expression pattern of gicerin in the regeneration of hepatocytes. Immunohistochemically, gicerin protein appeared in the regenerating hepatocytes of carbon tetrachloride (CCl 4 )-induced acute hepatitis, while it was scarcely expressed in the hepatocytes of normal mouse liver. Real-time PCR revealed the up-regulation of gicerin transcription in the regenerating process of CCl 4 -induced hepatitis. The expression of transforming growth factor (TGF)-ß1 was also increased during the regeneration. Furthermore, the gicerin mRNA expression increased during the process of an in vitro hepatocyte regeneration model using mouse primary hepatocytes and hepa 1-6 cells. To note, the mRNA levels of gicerin in these cells were enhanced by the presence of TGF-ß1. Collectively, these findings suggest that TGF-ß1 may therefore regulate gicerin expression in hepatocytes leading to liver regeneration by cell-cell or cell-ECM interactions.

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BT‐IgSF, a novel immunoglobulin superfamily protein, functions as a cell adhesion molecule

Harada

Suzu

Hayashi

et al. 2005

Journal Cellular Physiology

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BT-IgSF is a newly identified cell surface glycoprotein belonging to the immunoglobulin superfamily (IgSF). We have previously shown that the expression of the BT-IgSF gene was highly restricted to brain and testis, and its transcript was detected in both neurons and glial cells. In this study, to explore its function, we generated cells overexpressing BT-IgSF proteins and analyzed their phenotypes. We found that the constitutive expression of BT-IgSF in the myeloid leukemia cell line TF-1-fms did not alter the growth rates, but caused the formation of large cell aggregates. The cell aggregates were also observed with mutant BT-IgSF lacking its cytoplasmic tail, the amino acid sequences of which were highly conserved among the BT-IgSF subgroup proteins. The neutralizing antibody to beta(1) integrin did not diminish the cell aggregate formation. These results indicate that BT-IgSF functions as a cell adhesion molecule, that its cytoplasmic tail is not essential for the function, and that beta(1) integrin is not involved in the function. We confirmed the cell adhesion function using NIH/3T3 fibroblastic cells expressing BT-IgSF in an inducible system. Flow cytometric analyses with the cells demonstrated that the cell aggregation mediated by BT-IgSF was through homophilic molecular interaction, and in a Ca(2+)/Mg(2+)-independent manner. Coupled with its restricted pattern of the expression, the cell adhesion-inducing function of BT-IgSF suggests a role of the cell surface molecule in the development/function of the central nervous system and spermatogenesis.

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Gicerin, an Ig‐superfamily cell adhesion molecule, promotes the invasive and metastatic activities of a mouse fibroblast cell line

Cited by 8 publications

References 14 publications

Involvement of gicerin, a cell adhesion molecule, in the portal metastasis of rat colorectal adenocarcinoma cells

Involvement of gicerin, a cell adhesion molecule, in the portal metastasis of rat colorectal adenocarcinoma cells

Involvement of transforming growth factor-β in the expression of gicerin, a cell adhesion molecule, in the regeneration of hepatocytes

BT‐IgSF, a novel immunoglobulin superfamily protein, functions as a cell adhesion molecule

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